{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T21:38:49Z","timestamp":1791149929435,"version":"4.1.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2014,2,1]],"date-time":"2014-02-01T00:00:00Z","timestamp":1391212800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Bovine tuberculosis remains one of the most damaging diseases to agriculture, and there is also a concern for human spillover. A critical need exists for rapid, thorough, and inexpensive diagnostic methods capable of detecting and differentiating<jats:named-content content-type=\"genus-species\">Mycobacterium bovis<\/jats:named-content>infection from other pathogenic and environmental mycobacteria at multiple surveillance levels. In a previous study, Seth et al. (PLoS One 4:e5478, 2009,<jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"doi\" xlink:href=\"10.1371\/journal.pone.0005478\">doi:10.1371\/journal.pone.0005478<\/jats:ext-link>) identified 32 host peptides that specifically increased in the blood serum of<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>-infected animals). In the current study, 16<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>proteins were discovered in the blood serum proteomics data sets. A large-scale validation analysis was undertaken for selected host and<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>proteins using a cattle serum repository containing<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>(<jats:italic>n<\/jats:italic>= 128),<jats:named-content content-type=\"genus-species\">Mycobacterium kansasii<\/jats:named-content>(<jats:italic>n<\/jats:italic>= 10), and<jats:named-content content-type=\"genus-species\">Mycobacterium avium<\/jats:named-content>subsp.<jats:named-content content-type=\"genus-species\">paratuberculosis<\/jats:named-content>(<jats:italic>n<\/jats:italic>= 10), cases exposed to<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>(<jats:italic>n<\/jats:italic>= 424), and negative controls (<jats:italic>n<\/jats:italic>= 38). Of the host biomarkers, vitamin D binding protein (VDBP) showed the greatest sensitivity and specificity for<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>detection. Circulating<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>proteins, specifically polyketide synthetase 5, detected<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>-infected cattle with little to no seroreactivity against<jats:named-content content-type=\"genus-species\">M. kansasii<\/jats:named-content>- and<jats:named-content content-type=\"genus-species\">M. avium<\/jats:named-content>subsp.<jats:named-content content-type=\"genus-species\">paratuberculosis<\/jats:named-content>-infected animals. These data indicate that host and pathogen serum proteins can serve as reliable biomarkers for tracking<jats:named-content content-type=\"genus-species\">M. bovis<\/jats:named-content>infection in animal populations.<\/jats:p>","DOI":"10.1128\/jcm.02433-13","type":"journal-article","created":{"date-parts":[[2013,12,5]],"date-time":"2013-12-05T02:02:43Z","timestamp":1386208963000},"page":"536-543","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Circulating Mycobacterium bovis Peptides and Host Response Proteins as Biomarkers for Unambiguous Detection of Subclinical Infection"],"prefix":"10.1128","volume":"52","author":[{"given":"Elise A.","family":"Lamont","sequence":"first","affiliation":[{"name":"Department of Veterinary Population Medicine, University of Minnesota, St. Paul, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harish K.","family":"Janagama","sequence":"additional","affiliation":[{"name":"Department of Microbial and Molecular Pathogenesis, Texas A&amp;M Health Science Center, Bryan, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joao","family":"Ribeiro-Lima","sequence":"additional","affiliation":[{"name":"Department of Veterinary Population Medicine, University of Minnesota, St. Paul, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy","family":"Vulchanova","sequence":"additional","affiliation":[{"name":"Department of Veterinary Biomedical Sciences, University of Minnesota, St. Paul, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meetu","family":"Seth","sequence":"additional","affiliation":[{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"My","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Veterinary Population Medicine, University of Minnesota, St. Paul, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiran","family":"Kurmi","sequence":"additional","affiliation":[{"name":"Department of Veterinary Population Medicine, University of Minnesota, St. Paul, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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