{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:18:09Z","timestamp":1791303489783,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2011,6,1]],"date-time":"2011-06-01T00:00:00Z","timestamp":1306886400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Sepsis is a major health problem in newborns and children. Early detection of pathogens allows initiation of appropriate antimicrobial therapy that strongly correlates with positive outcomes. Multiplex PCR has the potential to rapidly identify bloodstream infections, compensating for the loss of blood culture sensitivity. In an Italian pediatric hospital, multiplex PCR (the LightCycler SeptiFast test) was compared to routine blood culture with 1,673 samples obtained from 803 children with suspected sepsis; clinical and laboratory information was used to determine the patient infection status. Excluding results attributable to contaminants, SeptiFast showed a sensitivity of 85.0% (95% confidence interval [CI] = 78.7 to 89.7%) and a specificity of 93.5% (95% CI = 92.1 to 94.7%) compared to blood culture. The rate of positive results was significantly higher with SeptiFast (14.6%) than blood culture (10.3%) (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001), and the overall positivity rate was 16.1% when the results of both tests were combined.\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            (11.6%), coagulase-negative staphylococci (CoNS) (29.6%),\n            <jats:named-content content-type=\"genus-species\">Pseudomonas aeruginosa<\/jats:named-content>\n            (16.5%), and\n            <jats:named-content content-type=\"genus-species\">Klebsiella<\/jats:named-content>\n            spp. (10.1%) were the most frequently detected. SeptiFast identified 97 additional isolates that blood culture failed to detect (24.7%\n            <jats:named-content content-type=\"genus-species\">P. aeruginosa<\/jats:named-content>\n            , 23.7% CoNS, 14.4%\n            <jats:named-content content-type=\"genus-species\">Klebsiella<\/jats:named-content>\n            spp., 14.4%\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            spp.). Among specimens taken from patients receiving antibiotic therapy, we also observed a significantly higher rate of positivity of SeptiFast than blood culture (14.1% versus 6.5%, respectively;\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001). On the contrary, contaminants were significantly more frequent among blood cultures than SeptiFast (\n            <jats:italic>n<\/jats:italic>\n            = 97 [5.8%] versus\n            <jats:italic>n<\/jats:italic>\n            = 26 [1.6%]), respectively;\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001). SeptiFast served as a highly valuable adjunct to conventional blood culture in children, adding diagnostic value and shortening the time to result (TTR) to 6 h.\n          <\/jats:p>","DOI":"10.1128\/jcm.02460-10","type":"journal-article","created":{"date-parts":[[2011,4,7]],"date-time":"2011-04-07T13:51:44Z","timestamp":1302184304000},"page":"2252-2258","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":147,"title":["Multiplex PCR Allows Rapid and Accurate Diagnosis of Bloodstream Infections in Newborns and Children with Suspected Sepsis"],"prefix":"10.1128","volume":"49","author":[{"given":"Barbara","family":"Lucignano","sequence":"first","affiliation":[{"name":"Unit of Microbiology, Children's Hospital and Research Institute Bambino Ges\u00f9, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Ranno","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, Children's Hospital and Research Institute Bambino Ges\u00f9, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Liesenfeld","sequence":"additional","affiliation":[{"name":"Roche Molecular Diagnostics, Pleasanton, California 94588"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatrice","family":"Pizzorno","sequence":"additional","affiliation":[{"name":"Roche Diagnostics S.p.A., Monza, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenza","family":"Putignani","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, Children's Hospital and Research Institute Bambino Ges\u00f9, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Bernaschi","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, Children's Hospital and Research Institute Bambino Ges\u00f9, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donato","family":"Menichella","sequence":"additional","affiliation":[{"name":"Unit of Microbiology, Children's Hospital and Research Institute Bambino Ges\u00f9, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.7.3119-3125.2003"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.032144"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-008-0672-6"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2006-0440"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.129.6.1432"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000298158.12101.41"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2006.09.014"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.PCC.0000149131.72248.E6"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00062-05"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199209000-00014"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0195941700063591"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.512-517.2004"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1385\/MB:22:3:231"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00147-10"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.3109\/00365549709008668"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2010.07.004"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1080\/1120009X.2007.11782441"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)01205-8"},{"key":"e_1_3_3_20_2","first-page":"9","article-title":"Comparison of broad range 16S rDNA PCR and conventional blood culture for diagnosis of sepsis in the newborn: a case control study","volume":"19","author":"Reier-Nilsen T.","year":"2009","unstructured":"Reier-NilsenT. 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