{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T10:49:05Z","timestamp":1776854945189,"version":"3.51.2"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Real-time detection of methicillin-resistant<jats:italic>Staphylococcus aureus<\/jats:italic>(MRSA) and methicillin-susceptible<jats:italic>S. aureus<\/jats:italic>(MSSA) in cases of clinical bacteremia may promote appropriate antimicrobial therapy and infection control. Expense inherent to molecular diagnostics may prevent laboratories from utilizing real-time PCR for this purpose. BD GeneOhm StaphSR assay master mix was reconstituted and aliquoted into SmartCycler tubes in 25-\u03bcl volumes (freshly reconstituted master mix), with a portion being frozen at \u221270\u00b0C (frozen master mix). Incubation of 40 previously analyzed lysates from positive BacT\/Alert SA and SN blood culture bottles (identified as 10 MRSA strains, 10 MSSA strains, 12 coagulase-negative<jats:italic>Staphylococcus<\/jats:italic>strains, and 8<jats:italic>Micrococcus<\/jats:italic>strains) in freshly reconstituted master mix and master mix frozen between 1 week and 6 months generated the expected results in all PCRs. Similarly, positive- and negative-control reagents stored frozen at \u221270\u00b0C for up to 18 weeks yielded the expected reactions. Prospective analysis of 244 positive blood culture samples utilizing 1-week-frozen master mix and freshly reconstituted master mix yielded a 1.2% discordant rate upon initial testing due to three unresolved results (two unresolved results for freshly reconstituted master mix and one unresolved result for frozen master mix). Repeat testing produced a final 100% concordance rate between the two master mix preparations. Use of master mix that was frozen up to 6 months did not compromise performance of the BD GeneOhm StaphSR assay. This modification, resulting in less reagent waste, may allow a greater number of laboratories to consider real-time PCR methodology for detection of bacteremia caused by MRSA and MSSA.<\/jats:p>","DOI":"10.1128\/jcm.02463-09","type":"journal-article","created":{"date-parts":[[2010,2,11]],"date-time":"2010-02-11T05:29:40Z","timestamp":1265866180000},"page":"1408-1412","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Cost-Effective Modification of a Commercial PCR Assay for Detection of Methicillin-Resistant or -Susceptible<i>Staphylococcus aureus<\/i>in Positive Blood Cultures"],"prefix":"10.1128","volume":"48","author":[{"given":"Erik","family":"Munson","sequence":"first","affiliation":[{"name":"Wheaton Franciscan Laboratory, Wauwatosa, Wisconsin 53226"},{"name":"College of Health Sciences, University of Wisconsin-Milwaukee, Milwaukee, Wisconsin 53201"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Timothy","family":"Kramme","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan Laboratory, Wauwatosa, Wisconsin 53226"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne","family":"Culver","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan Laboratory, Wauwatosa, Wisconsin 53226"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeanne E.","family":"Hryciuk","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan Laboratory, Wauwatosa, Wisconsin 53226"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald F.","family":"Schell","sequence":"additional","affiliation":[{"name":"Wisconsin State Laboratory of Hygiene, Madison, Wisconsin 53706"},{"name":"Departments of Microbiology"},{"name":"Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, Wisconsin 53706"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.md.0000091184.93122.09"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/533595"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/502522"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/345476"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.4.1219-1223.2006"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.8.3655-3660.2003"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/380457"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01414-06"},{"key":"e_1_3_2_10_2","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1002\/j.1875-9114.1997.tb03788.x","volume":"17","year":"1997","unstructured":"Gentry, C. 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