{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T08:25:00Z","timestamp":1774945500952,"version":"3.50.1"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2016,2,1]],"date-time":"2016-02-01T00:00:00Z","timestamp":1454284800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n Following analysis of primary cervix, vagina, and first-void female urine specimens for\n Chlamydia trachomatis<\/jats:named-content>\n ,\n Neisseria gonorrhoeae<\/jats:named-content>\n , and\n Trichomonas vaginalis<\/jats:named-content>\n via commercial transcription-mediated amplification (TMA), residual material was subjected to\n Mycoplasma genitalium<\/jats:named-content>\n research-use-only TMA. Representation within a 2,478-specimen retrospective study set was established by comparison to a 6-month audit of clinical\n C. trachomatis<\/jats:named-content>\n TMA (12,999 specimens) on the basis of the\n C. trachomatis<\/jats:named-content>\n detection rate, specimen source distribution, clinic location, and age.\n M. genitalium<\/jats:named-content>\n was detected in 282 (11.4%) patients. This rate was higher than those seen with\n T. vaginalis<\/jats:named-content>\n (9.0%;\n P<\/jats:italic>\n = 0.005),\n C. trachomatis<\/jats:named-content>\n (6.2%), and\n N. gonorrhoeae<\/jats:named-content>\n (1.4%). Positive\n M. genitalium<\/jats:named-content>\n results were confirmed by repeat testing or alternative-target TMA at a rate of 98.7%. The mean age of the\n M. genitalium<\/jats:named-content>\n -infected females (24.7 years) was lower than that of the\n T. vaginalis<\/jats:named-content>\n -infected females (mean, 30.1 years;\n P<\/jats:italic>\n < 0.0001) and higher than that of the\n C. trachomatis<\/jats:named-content>\n -infected females (mean, 23.8 years;\n P<\/jats:italic>\n = 0.003). Of 566 patient encounters positive for at least one sexually transmitted infection (STI), 35.9% exhibited sole detection of\n M. genitalium<\/jats:named-content>\n (\n P<\/jats:italic>\n \u2264 0.0004 versus sole detection of other STI agents) and 26.1% were solely positive for\n T. vaginalis<\/jats:named-content>\n (\n P<\/jats:italic>\n < 0.0002 versus\n C. trachomatis<\/jats:named-content>\n ). The\n M. genitalium<\/jats:named-content>\n and\n T. vaginalis<\/jats:named-content>\n detection rates among 755 patients at urban emergency departments were 14.6% and 13.0%, respectively (\n P<\/jats:italic>\n = 0.37). A 10.0%\n M. genitalium<\/jats:named-content>\n detection rate from other facilities exceeded that of\n T. vaginalis<\/jats:named-content>\n (7.2%;\n P<\/jats:italic>\n = 0.004). Incorporation of\n M. genitalium<\/jats:named-content>\n TMA into comprehensive testing programs would detect\n M. genitalium<\/jats:named-content>\n in a significant proportion of females, particularly those in outpatient obstetrics and gynecology (OB\/GYN) settings.\n <\/jats:p>","DOI":"10.1128\/jcm.02463-15","type":"journal-article","created":{"date-parts":[[2015,12,11]],"date-time":"2015-12-11T12:53:55Z","timestamp":1449838435000},"page":"432-438","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Clinical Laboratory Assessment of Mycoplasma genitalium Transcription-Mediated Amplification Using Primary Female Urogenital Specimens"],"prefix":"10.1128","volume":"54","author":[{"given":"Erik","family":"Munson","sequence":"first","affiliation":[{"name":"Wheaton Franciscan Laboratory, Milwaukee, Wisconsin, USA"},{"name":"College of Health Sciences, Marquette University, Milwaukee, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Holly","family":"Bykowski","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan Laboratory, Milwaukee, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimber L.","family":"Munson","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan Laboratory, Milwaukee, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maureen","family":"Napierala","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan Laboratory, Milwaukee, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela J.","family":"Reiss","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan Laboratory, Milwaukee, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald F.","family":"Schell","sequence":"additional","affiliation":[{"name":"Wisconsin State Laboratory of Hygiene, Madison, Wisconsin, USA"},{"name":"Department of Microbiology, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"},{"name":"Department of Medical Microbiology and Immunology, University of Wisconsin\u2014Madison, Madison, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeanne E.","family":"Hryciuk","sequence":"additional","affiliation":[{"name":"Wheaton Franciscan Laboratory, Milwaukee, Wisconsin, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00006-11"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir702"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2005.03.002"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2013.11.022"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0b013e32834ff690"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2013-051467"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/526501"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu644"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/civ312"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.4.1451-1455.2002"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.683-692.2004"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1086\/376990"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.746-752.2004"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00553-06"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.4.1236-1240.2006"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2015.03.016"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31815abac6"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3181b01948"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e318255de03"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2013.814429"},{"key":"e_1_3_2_22_2","unstructured":"APTIMACombo 2\u00ae Assay package insert. 2012. Gen-Probe Incorporated San Diego CA."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.304-309.2003"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01291-11"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00564-08"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02078-12"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00491-07"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2010.05.002"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00455-13"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-58"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.2105\/AJPH.2005.074062"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2006.022970"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001324"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3181bf5441"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2008.10.005"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05632-11"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02463-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02463-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T09:52:26Z","timestamp":1646473946000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02463-15"}},"subtitle":[],"editor":[{"given":"A. B.","family":"Onderdonk","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,2]]},"references-count":35,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,2]]}},"alternative-id":["10.1128\/JCM.02463-15"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02463-15","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2]]},"assertion":[{"value":"2015-09-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-12-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-01-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}