{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:18:11Z","timestamp":1791303491025,"version":"4.1.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T00:00:00Z","timestamp":1364774400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We conducted a case-control study using the Fungitell assay, the novel Platelia\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            Antigen (Ag) Plus and\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            Antibody (Ab) Plus assays, and the Cand-Tec latex agglutination test to evaluate the usefulness of (1\u21923)-\u03b2-\n            <jats:sc>d<\/jats:sc>\n            -glucan (BDG), mannan antigen with\/without anti-mannan antibody, and Cand-Tec\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            antigen measurement for the diagnosis of candidemia. A total of 56 patients fulfilled the inclusion criteria and were enrolled. One hundred patients with bacteremia and 100 patients with sterile blood cultures served as negative controls. In the candidemia group, median (1\u21923)-\u03b2-\n            <jats:sc>d<\/jats:sc>\n            -glucan, mannan antigen, and anti-mannan antibody levels were 427 pg\/ml, 190 pg\/ml, and 18.6 antibody units (AU)\/ml, respectively. All three parameters were significantly elevated in patients with candidemia. The sensitivity and specificity were, respectively, 87.5% and 85.5% for (1\u21923)-\u03b2-\n            <jats:sc>d<\/jats:sc>\n            -glucan, 58.9% and 97.5% for mannan antigen, 62.5% and 65.0% for anti-mannan antibody, 89.3% and 63.0% for mannan antigen plus anti-mannan antibody, 89.3% and 85.0% for BDG plus mannan antigen, and 13.0% and 93.9% for Cand-Tec\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            antigen. The low mannan antigen sensitivity was in part caused by\n            <jats:named-content content-type=\"genus-species\">Candida parapsilosis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Candida guilliermondii<\/jats:named-content>\n            fungemias, which were not detected by the Platelia\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            Ag Plus assay. When the cutoff was lowered from 125 pg\/ml to 50 pg\/ml, mannan antigen sensitivity increased to 69.6% without severely affecting the specificity (93.5%). Contrary to recently published data, superficial candidiasis was not associated with elevated mannan antigen levels, not even after the cutoff was lowered. Combining procalcitonin (PCT) with (1\u21923)-\u03b2-\n            <jats:sc>d<\/jats:sc>\n            -glucan to increase specificity provided a limited advantage because the benefit of the combination did not outweigh the loss of sensitivity. Our results demonstrate that the Cand-Tec\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            antigen and the mannan antigen plus anti-mannan antibody measurements have unacceptably low sensitivity or specificity. Of the four tests compared, (1\u21923)-\u03b2-\n            <jats:sc>d<\/jats:sc>\n            -glucan and mannan antigen are the superior biomarkers, depending on whether a sensitivity-driven or specificity-driven approach is used.\n          <\/jats:p>","DOI":"10.1128\/jcm.02473-12","type":"journal-article","created":{"date-parts":[[2013,1,31]],"date-time":"2013-01-31T03:54:41Z","timestamp":1359604481000},"page":"1158-1164","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":90,"title":["Comparison of (1\u21923)-\u03b2-\n            <scp>d<\/scp>\n            -Glucan, Mannan\/Anti-Mannan Antibodies, and Cand-Tec Candida Antigen as Serum Biomarkers for Candidemia"],"prefix":"10.1128","volume":"51","author":[{"given":"J\u00fcrgen","family":"Held","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, University Medical Centre Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Kohlberger","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, University Medical Centre Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elfriede","family":"Rappold","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, University Medical Centre Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Busse Grawitz","sequence":"additional","affiliation":[{"name":"Clinical Chemistry, University of Freiburg, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"H\u00e4cker","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hygiene, University Medical Centre Freiburg, Freiburg, 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