{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T21:29:43Z","timestamp":1791581383412,"version":"4.3.4"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81572077"],"award-info":[{"award-number":["81572077"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005088","name":"Beijing Municipal Bureau of Health","doi-asserted-by":"publisher","award":["XMLS20150"],"award-info":[{"award-number":["XMLS20150"]}],"id":[{"id":"10.13039\/501100005088","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005088","name":"Beijing Municipal Bureau of Health","doi-asserted-by":"publisher","award":["ZYLX201304"],"award-info":[{"award-number":["ZYLX201304"]}],"id":[{"id":"10.13039\/501100005088","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005088","name":"Beijing Municipal Bureau of Health","doi-asserted-by":"publisher","award":["2014-3-082"],"award-info":[{"award-number":["2014-3-082"]}],"id":[{"id":"10.13039\/501100005088","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005088","name":"Beijing Municipal Bureau of Health","doi-asserted-by":"publisher","award":["2014-3-083"],"award-info":[{"award-number":["2014-3-083"]}],"id":[{"id":"10.13039\/501100005088","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005088","name":"Beijing Municipal Bureau of Health","doi-asserted-by":"publisher","award":["2014-4-2161"],"award-info":[{"award-number":["2014-4-2161"]}],"id":[{"id":"10.13039\/501100005088","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005088","name":"Beijing Municipal Bureau of Health","doi-asserted-by":"publisher","award":["DFL20151501"],"award-info":[{"award-number":["DFL20151501"]}],"id":[{"id":"10.13039\/501100005088","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005081","name":"Beijing Council of Science and Technology","doi-asserted-by":"publisher","award":["D141107005214003"],"award-info":[{"award-number":["D141107005214003"]}],"id":[{"id":"10.13039\/501100005081","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Tuberculous pleurisy is one of the most common types of extrapulmonary tuberculosis, but its diagnosis remains difficult. In this study, we report for the first time on the detection of cell-free\n                    <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n                    DNA in pleural effusion and an evaluation of a newly developed molecular assay for the detection of cell-free\n                    <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n                    DNA. A total of 78 patients with pleural effusion, 60 patients with tuberculous pleurisy, and 18 patients with alternative diseases were included in this study. Mycobacterial culture, the Xpert MTB\/RIF assay, the adenosine deaminase assay, the T-SPOT.TB assay, and the cell-free\n                    <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n                    DNA assay were performed on all the pleural effusion samples. The cell-free\n                    <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n                    DNA assay and adenosine deaminase assay showed significantly higher sensitivities of 75.0% and 68.3%, respectively, than mycobacterial culture and the Xpert MTB\/RIF assay, which had sensitivities of 26.7% and 20.0%, respectively (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.01). All four of these tests showed good specificities: 88.9% for the adenosine deaminase assay and 100% for the remaining three assays. The T-SPOT.TB assay with pleural effusion showed the highest sensitivity of 95.0% but the lowest specificity of 38.9%. The cell-free\n                    <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n                    DNA assay detected as few as 1.25 copies of IS\n                    <jats:italic>6110<\/jats:italic>\n                    per ml of pleural effusion and showed good accordance of the results between repeated tests (\n                    <jats:italic>r<\/jats:italic>\n                    = 0.978,\n                    <jats:italic>P<\/jats:italic>\n                    = 2.84 \u00d7 10\n                    <jats:sup>\u221210<\/jats:sup>\n                    ). These data suggest that the cell-free\n                    <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n                    DNA assay is a rapid and accurate molecular test which provides direct evidence of\n                    <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n                    etiology.\n                  <\/jats:p>","DOI":"10.1128\/jcm.02473-16","type":"journal-article","created":{"date-parts":[[2017,3,8]],"date-time":"2017-03-08T20:50:34Z","timestamp":1489006234000},"page":"1526-1532","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":48,"title":["Rapid Detection of Cell-Free Mycobacterium tuberculosis DNA in Tuberculous Pleural Effusion"],"prefix":"10.1128","volume":"55","author":[{"given":"Nanying","family":"Che","sequence":"first","affiliation":[{"name":"Department of Pathology, Beijing Tuberculosis &amp; Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinting","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis, Beijing Tuberculosis &amp; Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zichen","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology, Beijing Tuberculosis &amp; Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology, Beijing Tuberculosis &amp; Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4179-1737","authenticated-orcid":false,"given":"Xiaoyou","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Tuberculosis, Beijing Tuberculosis &amp; Thoracic Tumor Research Institute, Beijing Chest Hospital, Capital Medical University, Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00408-009-9165-3"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.21037\/jtd.2016.05.87"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02182-15"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00103213"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.5588\/ijtld.13.0178"},{"key":"e_1_3_3_7_2","first-page":"3126","article-title":"Diagnosis of tuberculosis pleurisy with adenosine deaminase (ADA): a systematic review and meta-analysis","volume":"7","author":"Gui X","year":"2014","unstructured":"Gui X Xiao H . 2014. Diagnosis of tuberculosis pleurisy with adenosine deaminase (ADA): a systematic review and meta-analysis. Int J Clin Exp Med7:3126\u20133135.","journal-title":"Int J Clin Exp Med"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.rmed.2007.12.007"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.06-2273"},{"key":"e_1_3_3_10_2","first-page":"777","article-title":"Adenosine deaminase and interferon gamma measurements for the diagnosis of tuberculous pleurisy: a meta-analysis","volume":"7","author":"Greco S","year":"2003","unstructured":"Greco S Girardi E Masciangelo R Capoccetta GB Saltini C . 2003. Adenosine deaminase and interferon gamma measurements for the diagnosis of tuberculous pleurisy: a meta-analysis. Int J Tuberc Lung Dis7:777\u2013786.","journal-title":"Int J Tuberc Lung Dis"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.4238\/2015.September.28.19"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0083857"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2013.05.009"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00005309"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00823-15"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1843.2011.01941.x"},{"key":"e_1_3_3_17_2","first-page":"241","article-title":"Les acides nucl\u00e9iques du plasma sanguin chez l'homme","volume":"142","author":"Mandel P","year":"1948","unstructured":"Mandel P Metais P . 1948. Les acides nucl\u00e9iques du plasma sanguin chez l'homme. C R Seances Soc Biol Fil142:241\u2013243.","journal-title":"C R Seances Soc Biol Fil"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)02174-0"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2015.04.005"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2011.1114"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-010-0785-z"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2016.01.006"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1517494112"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0105221"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10555-016-9629-x"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0890-8508(91)90040-Q"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1086\/302205"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2013.203679"},{"key":"e_1_3_3_29_2","first-page":"3254","article-title":"Rapid clearance of plasma Epstein-Barr virus DNA after surgical treatment of nasopharyngeal carcinoma","volume":"9","author":"To EW","year":"2003","unstructured":"To EW Chan KC Leung SF Chan LY To KF Chan AT Johnson PJ Lo YM . 2003. Rapid clearance of plasma Epstein-Barr virus DNA after surgical treatment of nasopharyngeal carcinoma. Clin Cancer Res9:3254\u20133259.","journal-title":"Clin Cancer Res"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2004.08.002"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1843.2010.01723.x"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02473-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02473-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T05:02:07Z","timestamp":1646456527000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02473-16"}},"subtitle":[],"editor":[{"given":"Geoffrey A.","family":"Land","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,5]]},"references-count":30,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,5]]}},"alternative-id":["10.1128\/JCM.02473-16"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02473-16","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,5]]},"assertion":[{"value":"2016-12-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-04-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}