{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T07:59:02Z","timestamp":1791273542504,"version":"4.1.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Due to unacceptably high interlaboratory variation in caspofungin MIC values, we evaluated the use of micafungin as a surrogate marker to predict the susceptibility of<jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>spp. to caspofungin using reference methods and species-specific interpretive criteria. The MIC results for 3,764 strains of<jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>(eight species), including 73 strains with<jats:italic>fks<\/jats:italic>mutations, were used. Caspofungin MIC values and species-specific interpretive criteria were compared with those of micafungin to determine the percent categorical agreement (%CA) and very major error (VME), major error (ME), and minor error rates as well as their ability to detect<jats:italic>fks<\/jats:italic>mutant strains of<jats:named-content content-type=\"genus-species\">Candida albicans<\/jats:named-content>(11 mutants),<jats:named-content content-type=\"genus-species\">Candida tropicalis<\/jats:named-content>(4 mutants),<jats:named-content content-type=\"genus-species\">Candida krusei<\/jats:named-content>(3 mutants), and<jats:named-content content-type=\"genus-species\">Candida glabrata<\/jats:named-content>(55 mutants). Overall, the %CA was 98.8% (0.2% VMEs and MEs, 0.8% minor errors) using micafungin as the surrogate marker. Among the 60 isolates of<jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>(9 isolates),<jats:named-content content-type=\"genus-species\">C. tropicalis<\/jats:named-content>(5 isolates),<jats:named-content content-type=\"genus-species\">C. krusei<\/jats:named-content>(2 isolates), and<jats:named-content content-type=\"genus-species\">C. glabrata<\/jats:named-content>(44 isolates) that were nonsusceptible (either intermediate or resistant) to both caspofungin and micafungin, 54 (90.0%) contained a mutation in<jats:italic>fks1<\/jats:italic>or<jats:italic>fks2<\/jats:italic>. An additional 10<jats:named-content content-type=\"genus-species\">C. glabrata<\/jats:named-content>mutants, two<jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>mutants, and one mutant each of<jats:named-content content-type=\"genus-species\">C. tropicalis<\/jats:named-content>and<jats:named-content content-type=\"genus-species\">C. krusei<\/jats:named-content>were classified as susceptible to both antifungal agents. Using the epidemiological cutoff values (ECVs) of 0.12 \u03bcg\/ml for caspofungin and 0.03 \u03bcg\/ml for micafungin to differentiate wild-type (WT) from non-WT strains of<jats:named-content content-type=\"genus-species\">C. glabrata<\/jats:named-content>, 80% of the<jats:named-content content-type=\"genus-species\">C. glabrata<\/jats:named-content>mutants were non-WT for both agents (96% concordance). Micafungin may serve as an acceptable surrogate marker for the prediction of susceptibility and resistance of<jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>to caspofungin.<\/jats:p>","DOI":"10.1128\/jcm.02481-13","type":"journal-article","created":{"date-parts":[[2013,10,24]],"date-time":"2013-10-24T03:09:23Z","timestamp":1382584163000},"page":"108-114","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":57,"title":["Use of Micafungin as a Surrogate Marker To Predict Susceptibility and Resistance to Caspofungin among 3,764 Clinical Isolates of Candida by Use of CLSI Methods and Interpretive Criteria"],"prefix":"10.1128","volume":"52","author":[{"given":"Michael A.","family":"Pfaller","sequence":"first","affiliation":[{"name":"JMI Laboratories, North Liberty, Iowa, USA"},{"name":"University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shawn A.","family":"Messer","sequence":"additional","affiliation":[{"name":"JMI Laboratories, North Liberty, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel J.","family":"Diekema","sequence":"additional","affiliation":[{"name":"University of Iowa, Iowa City, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald N.","family":"Jones","sequence":"additional","affiliation":[{"name":"JMI Laboratories, North Liberty, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Castanheira","sequence":"additional","affiliation":[{"name":"JMI Laboratories, North Liberty, Iowa, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12039"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/596757"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12041"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01256-09"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00355-12"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01590-09"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01373-10"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00308-13"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2011.01.004"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2012.03880.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01292-08"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01364-10"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01519-13."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00566-08"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00443-09"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00998-09"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12281-011-0054-x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01102-12"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05872-11"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00621-11"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.06369-11"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit136"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1801.110556"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.06100-11"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01128-10"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02390-09"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00136-13"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00325-11"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01193-12"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00044-11"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2012.08194.x"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.7.3137-3141.2004"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01551-06"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01952-07"},{"key":"e_1_3_2_36_2","doi-asserted-by":"crossref","first-page":"1793","DOI":"10.1128\/9781555816728.ch115","volume-title":"Manual of clinical microbiology","author":"Howell SA","year":"2011","unstructured":"HowellSAHazenKC. 2011. 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