{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:07:20Z","timestamp":1790878040054,"version":"4.1.0"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Five clinical isolates of\n            <jats:italic>Nocardia<\/jats:italic>\n            that showed ambiguous bases within the variable region of the 16S rRNA gene sequence were evaluated for the presence of multiple copies of this gene. The type strains of three\n            <jats:italic>Nocardia<\/jats:italic>\n            species,\n            <jats:italic>Nocardia concava<\/jats:italic>\n            ,\n            <jats:italic>Nocardia ignorata<\/jats:italic>\n            , and\n            <jats:italic>Nocardia yamanashiensis<\/jats:italic>\n            , which also showed ambiguous bases in the variable region, were also examined. Cloning experiments using an amplified region of the 16S rRNA that contains the variable region showed that each isolate possessed 16S rRNA genes with at least two different sequences. In addition, hybridization studies using a 16S rRNA gene-specific probe and extracted genomic DNA of the patient isolates and of the type strain of\n            <jats:italic>N. ignorata<\/jats:italic>\n            showed that each isolate possessed at least three copies of the gene. These multiple differing copies of the 16S rRNA gene and the results of DNA-DNA hybridization studies indicate problems of species definition and identification for such isolates. A broader species concept than that currently in vogue may be required to accommodate such organisms.\n          <\/jats:p>","DOI":"10.1128\/jcm.02482-06","type":"journal-article","created":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T01:41:59Z","timestamp":1171503719000},"page":"1146-1151","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Analysis of Multiple Differing Copies of the 16S rRNA Gene in Five Clinical Isolates and Three Type Strains of\n            <i>Nocardia<\/i>\n            Species and Implications for Species Assignment"],"prefix":"10.1128","volume":"45","author":[{"given":"Patricia S.","family":"Conville","sequence":"first","affiliation":[{"name":"Microbiology Service, Department of Laboratory Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, U.S. Department of Health and Human Services, 10 Center Drive, MSC 1508, Bethesda, Maryland 20882-1508"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank G.","family":"Witebsky","sequence":"additional","affiliation":[{"name":"Microbiology Service, Department of Laboratory Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, U.S. Department of Health and Human Services, 10 Center Drive, MSC 1508, Bethesda, Maryland 20882-1508"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.18.4.816-824.1983"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.15.6.1133-1140.1982"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a025606"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.578-584.2004"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.11.5139-5145.2004"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2560-2568.2003"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.158-164.2000"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2881-2885.2005"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00155-06"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406410101"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02794-0"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63280-0"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.8.1880-1881.1989"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.10.2531-2536.1996"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.6.2530-2540.2004"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.6.1761-1764.1998"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.12.6167-6170.2005"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.4.817-822.1997"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.2.175-178.1993"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.10.3270-3276.1997"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-51-6-2127"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02482-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02482-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T05:49:37Z","timestamp":1646459377000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02482-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4]]},"references-count":21,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,4]]}},"alternative-id":["10.1128\/JCM.02482-06"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02482-06","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4]]},"assertion":[{"value":"2006-12-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-02-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}