{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:02:47Z","timestamp":1791176567802,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2014,2,1]],"date-time":"2014-02-01T00:00:00Z","timestamp":1391212800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Leptospirosis is a worldwide zoonosis which is responsible for the typical form of Weil's disease. The epidemiological surveillance of the\n            <jats:named-content content-type=\"genus-species\">Leptospira<\/jats:named-content>\n            species agent is important for host prevalence control. Although the genotyping methods have progressed, the identification of some serovars remains ambiguous. We investigated the multispacer sequence typing (MST) method for genotyping strains belonging to the species\n            <jats:named-content content-type=\"genus-species\">Leptospira interrogans<\/jats:named-content>\n            , which is the main agent of leptospirosis worldwide. A total of 33 DNA samples isolated from the reference strains of\n            <jats:named-content content-type=\"genus-species\">L. interrogans<\/jats:named-content>\n            serogroups Icterohaemorrhagiae, Australis, Canicola, and Grippotyphosa, which are the most prevalent serogroups in France, were analyzed by both the variable-number tandem-repeat (VNTR) and MST methods. An MST database has been constructed from the DNA of these reference strains to define the MST profiles. The MST profiles corroborated with the VNTR results. Moreover, the MST analysis allowed the identification at the serovar level or potentially to the isolate level for strains belonging to\n            <jats:named-content content-type=\"genus-species\">L. interrogans<\/jats:named-content>\n            serovar Icterohaemorrhagiae, which then results in a higher resolution than VNTR (Hunter-Gaston index of 0.94 versus 0.68). Regarding\n            <jats:named-content content-type=\"genus-species\">L. interrogans<\/jats:named-content>\n            serogroups Australis, Canicola, and Grippotyphosa, the MST and VNTR methods similarly identified the genotype. The MST method enabled the acquisition of simple and robust results that were based on the nucleotide sequences. The MST identified clinical isolates in correlation with the reference serovar profiles, thus permitting an epidemiological surveillance of circulating\n            <jats:named-content content-type=\"genus-species\">L. interrogans<\/jats:named-content>\n            strains, especially for the Icterohaemorrhagiae serogroup, which includes the most prevalent strains of public health interest.\n          <\/jats:p>","DOI":"10.1128\/jcm.02482-13","type":"journal-article","created":{"date-parts":[[2013,12,12]],"date-time":"2013-12-12T03:25:55Z","timestamp":1386818755000},"page":"564-571","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["High-Resolution Typing of Leptospira interrogans Strains by Multispacer Sequence Typing"],"prefix":"10.1128","volume":"52","author":[{"given":"Anne-Laure","family":"Zilber","sequence":"first","affiliation":[{"name":"USC1233 INRA\/VAS, Equipe PERS, Etablissement VetAgro Sup, Campus de Lyon, Marcy l'Etoile, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Picardeau","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unit\u00e9 de Biologie des Spiroch\u00e8tes, National Reference Center and WHO Collaborating Center for Leptospirosis, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Ayral","sequence":"additional","affiliation":[{"name":"USC1233 INRA\/VAS, Equipe PERS, Etablissement VetAgro Sup, Campus de Lyon, Marcy l'Etoile, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc","family":"Artois","sequence":"additional","affiliation":[{"name":"USC1233 INRA\/VAS, Equipe PERS, Etablissement VetAgro Sup, Campus de Lyon, Marcy l'Etoile, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Demont","sequence":"additional","affiliation":[{"name":"Laboratoire des Leptospires, Etablissement VetAgro Sup, Campus de Lyon, Marcy l'Etoile, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angeli","family":"Kodjo","sequence":"additional","affiliation":[{"name":"USC1233 INRA\/VAS, Equipe PERS, Etablissement VetAgro Sup, Campus de Lyon, Marcy l'Etoile, France"},{"name":"Laboratoire des Leptospires, Etablissement VetAgro Sup, Campus de Lyon, Marcy l'Etoile, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoheira","family":"Djelouadji","sequence":"additional","affiliation":[{"name":"USC1233 INRA\/VAS, Equipe PERS, Etablissement VetAgro Sup, Campus de Lyon, Marcy l'Etoile, France"},{"name":"Laboratoire des Leptospires, Etablissement VetAgro Sup, Campus de Lyon, Marcy l'Etoile, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2009.06.009"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03474.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cair.2004.08.001"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00830-2"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000228"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.2.296-326.2001"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.03.012"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2010.0654.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-48-3-851"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.047233-0"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268812002087"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2012.10.002"},{"key":"e_1_3_2_14_2","volume-title":"Human leptospirosis: guidance for diagnosis, surveillance and control","author":"WHO","year":"2003","unstructured":"WHO. 2003. 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