{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T19:12:17Z","timestamp":1791141137434,"version":"4.1.0"},"reference-count":8,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2007,9,1]],"date-time":"2007-09-01T00:00:00Z","timestamp":1188604800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Although transmissible metallo-\u03b2-lactamases (MBLs) are a serious threat to \u03b2-lactam antibiotic therapy, the CLSI currently does not recommend testing methods for the detection of MBLs. The aim of this study was to evaluate the capability of double-disk tests (DDTs) by using disks containing a combination of the chelators 2-mercaptopropionic acid (MPA) and Tris-EDTA (TE) to detect MBLs. Sixteen isolates (4\n            <jats:italic>Acinetobacter baumannii<\/jats:italic>\n            isolates, 6\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            isolates, 1\n            <jats:italic>Serratia marcescens<\/jats:italic>\n            isolate, 1\n            <jats:italic>Aeromonas hydrophila<\/jats:italic>\n            isolate, 1\n            <jats:italic>Aeromonas veronii<\/jats:italic>\n            isolate, 2\n            <jats:italic>Chryseobacterium meningosepticum<\/jats:italic>\n            isolates, and 1\n            <jats:italic>Stenotrophomonas maltophilia<\/jats:italic>\n            isolate) producing IMP-1, IMP-1-like, IMP-18, GIM-1, SPM-1, VIM-2, VIM-2-like, and chromosomal MBLs and 20 isolates (7\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            isolates, 3\n            <jats:italic>Escherichia coli<\/jats:italic>\n            isolates, 5\n            <jats:italic>Enterobacter cloacae<\/jats:italic>\n            isolates, 2\n            <jats:italic>S. marcescens<\/jats:italic>\n            isolates, 1\n            <jats:italic>Proteus mirabilis<\/jats:italic>\n            isolate, and 2\n            <jats:italic>A. baumannii<\/jats:italic>\n            isolates) producing non-MBL carbapenemases, AmpC \u03b2-lactamases, and extended-spectrum \u03b2-lactamases were tested. The DDT method was evaluated by using four types of chelator disks (TE, high-strength TE, MPA, and TE plus 20 \u03bcl of MPA [at various concentrations]) and the \u03b2-lactams imipenem (IPM), meropenem (MEM), ertapenem (ERT), and ceftazidime (CAZ). DDTs with IPM and a TE disk supplemented with 1:320 MPA detected all MBLs and yielded no false-positive results. Some, but not all, MBL producers were detected in IPM-based tests involving the single chelator TE or MPA alone or by ERT- or CAZ-based tests. IPM-based tests with MPA concentrations other than 1:320 and all MEM-based tests had suboptimal sensitivities or specificities. DDT with IPM and a TE disk supplemented with 20 \u03bcl of 1:320 MPA appears to be convenient for the detection of MBLs in the clinical laboratory.\n          <\/jats:p>","DOI":"10.1128\/jcm.02486-06","type":"journal-article","created":{"date-parts":[[2007,6,28]],"date-time":"2007-06-28T04:42:48Z","timestamp":1183005768000},"page":"2798-2801","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Convenient Test Using a Combination of Chelating Agents for Detection of Metallo-\u03b2-Lactamases in the Clinical Laboratory"],"prefix":"10.1128","volume":"45","author":[{"given":"Soo-Young","family":"Kim","sequence":"first","affiliation":[{"name":"Center for Research in Anti-Infectives and Biotechnology, Department of Medical Microbiology &amp; Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, Nebraska 68178"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong Geun","family":"Hong","sequence":"additional","affiliation":[{"name":"Center for Research in Anti-Infectives and Biotechnology, Department of Medical Microbiology &amp; Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, Nebraska 68178"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ellen S.","family":"Moland","sequence":"additional","affiliation":[{"name":"Center for Research in Anti-Infectives and Biotechnology, Department of Medical Microbiology &amp; Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, Nebraska 68178"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth S.","family":"Thomson","sequence":"additional","affiliation":[{"name":"Center for Research in Anti-Infectives and Biotechnology, Department of Medical Microbiology &amp; Immunology, Creighton University School of Medicine, 2500 California Plaza, Omaha, Nebraska 68178"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.40-43.2000"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0691.2001.00204.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.10.4623-4629.2003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0691.2002.00401.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki265"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.8.2755-2759.2002"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.306-325.2005"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.10.3798-3801.2002"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02486-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02486-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:04:35Z","timestamp":1646460275000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02486-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9]]},"references-count":8,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1128\/JCM.02486-06"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02486-06","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,9]]},"assertion":[{"value":"2006-12-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-06-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}