{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T19:02:11Z","timestamp":1785178931888,"version":"3.55.0"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2008,5,1]],"date-time":"2008-05-01T00:00:00Z","timestamp":1209600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Trichomonas vaginalis<\/jats:italic>\n            is an important pathogen in both men and women. Culture is considered the diagnostic gold standard, although studies have shown that PCR is more sensitive than either culture or wet mount for the diagnosis of\n            <jats:italic>T. vaginalis<\/jats:italic>\n            infections. We sought to identify a simple method for stabilizing\n            <jats:italic>T. vaginalis<\/jats:italic>\n            DNA in urine samples that could be easily applied to molecular testing. The stability of\n            <jats:italic>T. vaginalis<\/jats:italic>\n            DNA in 40 urine samples was assessed by storage for various times at either 4\u00b0C or room temperature with or without the Becton Dickinson urine preservative transport (UPT) kit. Overall, there was better stability of\n            <jats:italic>T. vaginalis<\/jats:italic>\n            DNA when specimens were stored at 4\u00b0C than when they were stored at 20 to 22\u00b0C and when the UPT system was used.\n            <jats:italic>T. vaginalis<\/jats:italic>\n            DNA was stable in specimens stored without using the UPT at 4\u00b0C for about 3 days and at room temperature for only 1 day. For specimens placed in the UPT within 24 h (times of 1, 6, and 24 h) of collection, the DNA was stable for up to 30 days when stored at 4\u00b0C. For specimens stored at room temperature, the urine should be added to the UPT ideally within 1 hour of collection, and in this case the DNA remained stable for up to 30 days. When storing specimens at room temperature, a delay of 24 h prior to adding to UPT led to an unacceptably high loss of assay sensitivity.\n          <\/jats:p>","DOI":"10.1128\/jcm.02486-07","type":"journal-article","created":{"date-parts":[[2008,3,13]],"date-time":"2008-03-13T00:49:37Z","timestamp":1205369377000},"page":"1628-1630","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Stability of\n            <i>Trichomonas vaginalis<\/i>\n            DNA in Urine Specimens"],"prefix":"10.1128","volume":"46","author":[{"given":"Jessica","family":"Ingersoll","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tameka","family":"Bythwood","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Abdul-Ali","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gina M.","family":"Wingood","sequence":"additional","affiliation":[{"name":"Department of Behavioral Science, Rollins School of Public Health, Atlanta, Georgia"},{"name":"Center for AIDS Research, Emory University, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralph J.","family":"Diclemente","sequence":"additional","affiliation":[{"name":"Department of Behavioral Science, Rollins School of Public Health, Atlanta, Georgia"},{"name":"Center for AIDS Research, Emory University, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela M.","family":"Caliendo","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia"},{"name":"Center for AIDS Research, Emory University, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/10647440500068248"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199707000-00008"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003081-199303000-00020"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.11.3819-3822.2001"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-199301000-00015"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.11.3205-3210.1998"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/338027"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2003.tb00104.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.78.5.378"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01287-06"},{"key":"e_1_3_2_12_2","first-page":"213","volume":"351","year":"1998","unstructured":"Sorvillo, F., and P. Kerndt. 1998. Trichomonas vaginalis and amplification of HIV-1 transmission. Lancet351:213-214.","journal-title":"Lancet"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.12.4127-4130.1999"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1363\/3600604"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/342060"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-200106000-00003"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02486-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02486-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:25:22Z","timestamp":1646461522000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02486-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,5]]},"references-count":15,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2008,5]]}},"alternative-id":["10.1128\/JCM.02486-07"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02486-07","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,5]]},"assertion":[{"value":"2007-12-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-03-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}