{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T13:05:05Z","timestamp":1791119105300,"version":"4.1.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2007,6,1]],"date-time":"2007-06-01T00:00:00Z","timestamp":1180656000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Bacillus anthracis<\/jats:italic>\n            , the etiologic agent of anthrax, has been widely described as a genetically monomorphic species. We used both multiple-locus variable-number tandem-repeat analysis (MLVA) and\n            <jats:italic>pagA<\/jats:italic>\n            gene sequencing to determine the genetic diversity of a historical collection of\n            <jats:italic>B. anthracis<\/jats:italic>\n            isolates collected from the 1950s to the 1980s from various geographic locations and sources. We sequenced the\n            <jats:italic>pagA<\/jats:italic>\n            gene of 124 diverse\n            <jats:italic>B. anthracis<\/jats:italic>\n            isolates and found all previously identified\n            <jats:italic>B. anthracis pagA<\/jats:italic>\n            types except type 4. Sixty-three of the 124\n            <jats:italic>B. anthracis<\/jats:italic>\n            strains were identified as\n            <jats:italic>pagA<\/jats:italic>\n            type 6, while 44 were\n            <jats:italic>pagA<\/jats:italic>\n            type 5, 12 were\n            <jats:italic>pagA<\/jats:italic>\n            type 1, and individual isolates were identified for types 2 and 3, respectively. Two new\n            <jats:italic>pagA<\/jats:italic>\n            genotypes were discovered in three environmental isolates within the historical collection. Two isolates had the same new genotype, and an additional isolate produced a second new genotype. MLVA detected 22 previously described genotypes in the historical collection. In addition, 33 new MLVA genotypes were found. For 11 isolates, an MLVA genotype could not be assigned because one or more alleles did not amplify. While only two additional\n            <jats:italic>B. anthracis pagA<\/jats:italic>\n            types were identified, in two instances, the use of\n            <jats:italic>pagA<\/jats:italic>\n            sequencing discriminated isolates with the same MLVA genotype. MLVA revealed that 39 of the 124 isolates were previously undocumented genotypes and that 1 isolate was found to be in the C cluster when it was subtyped by MLVA.\n          <\/jats:p>","DOI":"10.1128\/jcm.02488-06","type":"journal-article","created":{"date-parts":[[2007,3,29]],"date-time":"2007-03-29T00:49:32Z","timestamp":1175129372000},"page":"1777-1782","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Genetic Diversity in a\n            <i>Bacillus anthracis<\/i>\n            Historical Collection (1954 to 1988)"],"prefix":"10.1128","volume":"45","author":[{"given":"David","family":"Sue","sequence":"first","affiliation":[{"name":"Bacterial Zoonoses Branch, Division of Foodborne, Bacterial and Mycotic Diseases, National Center for Zoonotic, Vector-Borne and Enteric Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd., NE, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chung K.","family":"Marston","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses Branch, Division of Foodborne, Bacterial and Mycotic Diseases, National Center for Zoonotic, Vector-Borne and Enteric Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd., NE, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex R.","family":"Hoffmaster","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses Branch, Division of Foodborne, Bacterial and Mycotic Diseases, National Center for Zoonotic, Vector-Borne and Enteric Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd., NE, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia P.","family":"Wilkins","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses Branch, Division of Foodborne, Bacterial and Mycotic Diseases, National Center for Zoonotic, Vector-Borne and Enteric Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd., NE, Atlanta, Georgia 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