{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:13:56Z","timestamp":1791288836123,"version":"4.3.0"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2009,5,1]],"date-time":"2009-05-01T00:00:00Z","timestamp":1241136000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Burkholderia gladioli<\/jats:italic>\n            , primarily known as a plant pathogen, is involved in human infections, especially in patients with cystic fibrosis (CF). In the present study, the first respiratory isolates recovered from 14 French patients with CF and 4 French patients without CF, identified by 16S rRNA gene analysis, were tested for growth on\n            <jats:italic>B. cepacia<\/jats:italic>\n            selective media, for identification by commercial systems, and for their antimicrobial susceptibilities, and were compared by pulsed-field gel electrophoresis (PFGE). Patients' data were collected. All 18 isolates grew on oxidation-fermentation-polymyxin B-bacitracin-lactose medium and\n            <jats:italic>Pseudomonas cepacia<\/jats:italic>\n            agar, but only 13 grew on\n            <jats:italic>Burkholderia cepacia<\/jats:italic>\n            selective agar. API 20NE strips did not differentiate\n            <jats:italic>B. gladioli<\/jats:italic>\n            from\n            <jats:italic>B. cepacia<\/jats:italic>\n            , whereas Vitek 2 GN cards correctly identified 15 isolates. All isolates were susceptible to piperacillin, imipenem, aminoglycosides, and ciprofloxacin and were far less resistant to ticarcillin than\n            <jats:italic>B. cepacia<\/jats:italic>\n            complex organisms. Fifteen PFGE types were observed among the 18 isolates, but shared types were not identified among epidemiologically related patients. The microbiological follow-up of CF patients showed that colonization was persistent in 3 of 13 documented cases;\n            <jats:italic>B. gladioli<\/jats:italic>\n            was isolated from posttransplantation cultures of blood from 1 patient. Among the patients without CF,\n            <jats:italic>B. gladioli<\/jats:italic>\n            was associated with intubation (three cases) or bronchiectasis (one case). In summary, the inclusion of\n            <jats:italic>B. gladioli<\/jats:italic>\n            in the databases of commercial identification systems should improve the diagnostic capabilities of those systems. In CF patients, this organism is more frequently involved in transient infections than in chronic infections, but it may be responsible for complications posttransplantation; patient-to-patient transmission has not been demonstrated to date. Lastly,\n            <jats:italic>B. gladioli<\/jats:italic>\n            appears to be naturally susceptible to aminoglycosides and ciprofloxacin, although resistant isolates may emerge in the course of chronic infections.\n          <\/jats:p>","DOI":"10.1128\/jcm.02489-08","type":"journal-article","created":{"date-parts":[[2009,3,19]],"date-time":"2009-03-19T01:44:24Z","timestamp":1237427064000},"page":"1510-1516","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Microbiological and Epidemiological Features of Clinical Respiratory Isolates of\n            <i>Burkholderia gladioli<\/i>"],"prefix":"10.1128","volume":"47","author":[{"given":"Christine","family":"Segonds","sequence":"first","affiliation":[{"name":"Observatoire Burkholderia cepacia, Laboratoire de Bacte\u0301riologie-Hygie\u0300ne, Ho\u0302pital Purpan, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Clavel-Batut","sequence":"additional","affiliation":[{"name":"Observatoire Burkholderia cepacia, Laboratoire de Bacte\u0301riologie-Hygie\u0300ne, Ho\u0302pital Purpan, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Thouverez","sequence":"additional","affiliation":[{"name":"Laboratoire de Bacte\u0301riologie, Ho\u0302pital Jean Minjoz, Besanc\u0327on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Grenet","sequence":"additional","affiliation":[{"name":"Service de Pneumologie, Ho\u0302pital Foch, Suresnes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alain","family":"Le Coustumier","sequence":"additional","affiliation":[{"name":"Laboratoire de Biologie, Centre Hospitalier, Cahors, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Ple\u0301siat","sequence":"additional","affiliation":[{"name":"Laboratoire de Bacte\u0301riologie, Ho\u0302pital Jean Minjoz, Besanc\u0327on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ge\u0301rard","family":"Chabanon","sequence":"additional","affiliation":[{"name":"Observatoire Burkholderia cepacia, Laboratoire de Bacte\u0301riologie-Hygie\u0300ne, Ho\u0302pital Purpan, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-60-2-199"},{"key":"e_1_3_2_3_2","first-page":"123","volume":"23","year":"1997","unstructured":"Barker, P., R. Wood, and P. Gilligan. 1997. Lung infection with Burkholderia gladioli in a child with cystic fibrosis: acute clinical and spirometric deterioration. Pediatr. Pulmonol.23:123-125.","journal-title":"Pediatr. Pulmonol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.9.2748-2751.1998"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000177285.44374.dc"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.2.270-273.1989"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.52.3.173"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.160.1.16011"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-50-6-2135"},{"key":"e_1_3_2_10_2","first-page":"37","volume":"49","year":"1999","unstructured":"Coenye, T., B. Holmes, K. Kersters, J. R. W. Govan, and P. Vandamme. 1999. Burkholderia cocovenenans (van Damme et al. 1960) Gillis et al. 1995 andBurkholderia vandii Urakami et al. 1993 are junior synonyms of Burkholderia gladioli (Severini 1913) and Burkholderia plantarii (Azegami et al. 1987) Urakami et al. 1994, respectively. Int. J. Syst. Bacteriol.49:37-42.","journal-title":"1960) Gillis et al. 1995 and"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.16.5.622"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.10.3793-3797.2002"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1086\/515551"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.4.1004-1007.1999"},{"key":"e_1_3_2_15_2","first-page":"915","volume":"7","year":"2003","unstructured":"Jiao, Z., Y. Kawamura, N. Mishima, R. Yang, N. Li, X. Liu, and T. Ezaki. 2003. Need to differentiate lethal toxin-producing strains of Burkholderia gladioli, which cause severe food poisoning: description of B. gladioli pathovar cocovenenans and an emended description of B. gladioli. Microbiol. Immunol.7:915-925.","journal-title":"Microbiol. 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