{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T07:55:53Z","timestamp":1789631753822,"version":"build-2803163510"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We evaluated the usefulness of an\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            galactomannan (GM) test, a \u03b2-\n            <jats:sc>d<\/jats:sc>\n            -glucan (\u03b2DG) test, and two different\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            PCR assays of bronchoalveolar lavage fluid (BALF) samples for the diagnosis of chronic pulmonary aspergillosis (CPA). BALF samples from 30 patients with and 120 patients without CPA were collected. We calculated the sensitivity, specificity, positive predictive value, negative predictive value, positive likelihood ratio, negative likelihood ratio, and diagnostic odds ratio for each test individually and in combination with other tests. The optical density index values, as determined by receiver operating characteristic analysis, for the diagnosis of CPA were 0.5 and 100 for GM and \u03b2DG testing of BALF, respectively. The sensitivity and specificity of the GM test, \u03b2DG test, and PCR assays 1 and 2 were 77.8% and 90.0%, 77.8% and 72.5%, 86.7% and 84.2%, and 66.7% and 94.2%, respectively. A comparison of the PCR assays showed that PCR assay 1 had a better sensitivity, a better negative predictive value, and a better negative likelihood ratio and PCR assay 2 had a better specificity, a better positive predictive value, and a better positive likelihood ratio. The combination of the GM and \u03b2DG tests had the highest diagnostic odds ratio. The combination of the GM and \u03b2DG tests on BALF was more useful than any single test for diagnosing CPA.\n          <\/jats:p>","DOI":"10.1128\/jcm.02497-16","type":"journal-article","created":{"date-parts":[[2017,3,23]],"date-time":"2017-03-23T00:34:40Z","timestamp":1490229280000},"page":"1738-1746","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Usefulness of Two Aspergillus PCR Assays and Aspergillus Galactomannan and \u03b2-\n            <scp>d<\/scp>\n            -Glucan Testing of Bronchoalveolar Lavage Fluid for Diagnosis of Chronic Pulmonary Aspergillosis"],"prefix":"10.1128","volume":"55","author":[{"given":"Naohisa","family":"Urabe","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine, Toho University Omori Medical Center, Tokyo, 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