{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T12:55:16Z","timestamp":1787748916106,"version":"build-2784847793"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Recent studies of\n            <jats:named-content content-type=\"genus-species\">Toxoplasma gondii<\/jats:named-content>\n            isolates from animals in Brazil have revealed high genetic diversity. Many of these isolates are virulent to mice. It is speculated that these isolates may also be virulent to humans. However, there is very limited data regarding\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            strains from human infection. Therefore, it is not clear whether there is any association between parasite genotypes and disease phenotypes. In this study, a total of 27\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            strains were isolated from humans with congenital toxoplasmosis in Minas Gerais state, Brazil. The genetic variability was assessed by restricted fragment length polymorphism in 11 loci (\n            <jats:italic>SAG1<\/jats:italic>\n            , 5\u2032 plus 3\u2032\n            <jats:italic>SAG2<\/jats:italic>\n            , alternative [alt.]\n            <jats:italic>SAG2<\/jats:italic>\n            ,\n            <jats:italic>SAG3<\/jats:italic>\n            ,\n            <jats:italic>BTUB<\/jats:italic>\n            ,\n            <jats:italic>GRA6<\/jats:italic>\n            ,\n            <jats:italic>c22-8<\/jats:italic>\n            ,\n            <jats:italic>c29-2<\/jats:italic>\n            ,\n            <jats:italic>L358<\/jats:italic>\n            ,\n            <jats:italic>PK1<\/jats:italic>\n            , and\n            <jats:italic>Apico<\/jats:italic>\n            ). Genetic analysis of 24 strains revealed 14 different genotypes, including 7 previously identified from animals and 7 new types. The widespread genotype BrII accounted for 29% (7\/24) of the isolates and was the dominant genotype involved in this study. This is the first report of genotyping of\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            isolates obtained from blood samples from newborns with congenital toxoplasmosis. Genotypic characterization of these isolates suggests high genetic diversity of\n            <jats:named-content content-type=\"genus-species\">T. gondii<\/jats:named-content>\n            in this human population in Brazil. Future studies are needed to determine the source of contamination of this human population.\n          <\/jats:p>","DOI":"10.1128\/jcm.02502-12","type":"journal-article","created":{"date-parts":[[2013,1,3]],"date-time":"2013-01-03T07:24:24Z","timestamp":1357197864000},"page":"901-907","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":131,"title":["Genetic Characterization of Toxoplasma gondii Revealed Highly Diverse Genotypes for Isolates from Newborns with Congenital Toxoplasmosis in Southeastern Brazil"],"prefix":"10.1128","volume":"51","author":[{"given":"Ana Carolina Aguiar Vasconcelos","family":"Carneiro","sequence":"first","affiliation":[{"name":"Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gl\u00e1ucia Manzan","family":"Andrade","sequence":"additional","affiliation":[{"name":"Departamento de Pediatria, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00falia Gatti Ladeia","family":"Costa","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Breno Veloso","family":"Pinheiro","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Vitor","family":"Vasconcelos-Santos","sequence":"additional","affiliation":[{"name":"Departamento de Oftalmologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana Melo","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlei","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Tennessee, Knoxville, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 N\u00e9lio","family":"Janu\u00e1rio","sequence":"additional","affiliation":[{"name":"N\u00facleo de A\u00e7\u00f5es e Pesquisa em Apoio Diagn\u00f3stico, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Wagner Almeida","family":"Vitor","sequence":"additional","affiliation":[{"name":"Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(00)00124-7"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1017\/S095026880400353X"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762004000100002"},{"key":"e_1_3_3_5_2","first-page":"21","article-title":"Hearing loss in congenital toxoplasmosis detected by newborn screening","volume":"74","author":"Queiroz GM","year":"2008","unstructured":"QueirozGM ResendeLM GoulartEMA SiqueiraAL VitorRWA Janu\u00e1rioJN. 2008. 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