{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T19:19:19Z","timestamp":1782933559550,"version":"3.54.5"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T00:00:00Z","timestamp":1309478400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Drug resistance in tuberculosis (TB) is a matter of grave concern for TB control programs, as there is currently no cure for some extensively drug-resistant (XDR) strains. There is concern that this resistance could transmit, stressing the need for additional control measures, rapid diagnostic methods, and newer drugs for treatment. We developed an in-house assay that can rapidly detect resistance to drugs involved in the definition of XDR-TB directly from smear-positive specimens. Two hundred fifteen phenotypically XDR-TB isolates and 50 pansusceptible isolates were analyzed using a reverse line blot hybridization (RLBH) assay. The assay was also successfully applied to 73 smear-positive clinical specimens. The RLBH assay exhibited good sensitivity for the detection of resistance to isoniazid (99%), rifampin (99%), fluoroquinolones (95.3%), and second-line aminoglycosides (94.8%). The results from application of this assay on direct smear-positive clinical specimens revealed 93% concordance with the phenotypic drug susceptibility test (DST) results for the above-mentioned drugs. The time to accurate DST results was significantly reduced from weeks to 3 days. This molecular assay is a highly accurate tool for screening for XDR-TB, which achieves a substantial reduction in diagnostic delays.<\/jats:p>","DOI":"10.1128\/jcm.02511-10","type":"journal-article","created":{"date-parts":[[2011,5,26]],"date-time":"2011-05-26T05:21:46Z","timestamp":1306387306000},"page":"2546-2551","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["Rapid Diagnosis of Extensively Drug-Resistant Tuberculosis by Use of a Reverse Line Blot Hybridization Assay"],"prefix":"10.1128","volume":"49","author":[{"given":"Kanchan","family":"Ajbani","sequence":"first","affiliation":[{"name":"Department of Research, P. D. Hinduja National Hospital and Medical Research Centre, Mumbai, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anjali","family":"Shetty","sequence":"additional","affiliation":[{"name":"Department of Microbiology, P. D. Hinduja National Hospital and Medical Research Centre, Mumbai, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ajita","family":"Mehta","sequence":"additional","affiliation":[{"name":"Department of Microbiology, P. D. Hinduja National Hospital and Medical Research Centre, Mumbai, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camilla","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Microbiology, P. D. Hinduja National Hospital and Medical Research Centre, Mumbai, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00113-11"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.8.1700"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0907847"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60410-2"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00671-6"},{"key":"e_1_3_3_7_2","unstructured":"KentP. T.\nKubicaG. P.. 1985. Public health mycobacteriology: a guide for the level III laboratory p. 64-68. U.S. Department of Health and Human Services Public Health Service Centers for Disease Control Atlanta GA."},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00661-10"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00201-10"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.10.2521-2525.1997"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.8.3192-3197.2005"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2004.02.006"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.8.4.496"},{"key":"e_1_3_3_14_2","first-page":"217","article-title":"Evaluation of the BACTEC MGIT 960 system for the recovery and identification of Mycobacterium tuberculosis complex in a high throughput tertiary care centre","volume":"27","author":"Rodrigues C.","year":"2009","unstructured":"RodriguesC.. 2009. Evaluation of the BACTEC MGIT 960 system for the recovery and identification of Mycobacterium tuberculosis complex in a high throughput tertiary care centre. Int J. Med. Microbiol. 27:217\u2013221.","journal-title":"Int J. Med. Microbiol."},{"key":"e_1_3_3_15_2","first-page":"46","article-title":"Rapid speciation of 15 clinically relevant mycobacteria with simultaneous detection of resistance to rifampin, isoniazid, and streptomycin in Mycobacterium tuberculosis. Int. J","volume":"13","author":"Shenai S.","year":"2009","unstructured":"ShenaiS. RodriguesC. MehtaA.. 2009. Rapid speciation of 15 clinically relevant mycobacteria with simultaneous detection of resistance to rifampin, isoniazid, and streptomycin in Mycobacterium tuberculosis. Int. J. Infect. Dis. 13:46\u201358.","journal-title":"Infect. Dis."},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2996-2997.2005"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.5.1220-1225.1998"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.4.773"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.3.719-723.1997"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.9.3194-3199.2000"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000757"},{"key":"e_1_3_3_22_2","first-page":"838","article-title":"Timely diagnosis of MDR-TB under program conditions: is rapid drug susceptibility testing sufficient?","volume":"10","author":"Yagui M.","year":"2006","unstructured":"YaguiM.. 2006. Timely diagnosis of MDR-TB under program conditions: is rapid drug susceptibility testing sufficient? Int. J. Tuberc. Lung Dis. 10:838\u2013843.","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4438-4443.2004"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.11.5477-5482.2005"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.14.3929"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02511-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02511-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:56:49Z","timestamp":1646485009000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02511-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,7]]},"references-count":24,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2011,7]]}},"alternative-id":["10.1128\/JCM.02511-10"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02511-10","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,7]]},"assertion":[{"value":"2010-12-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-05-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-06-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}