{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T07:01:24Z","timestamp":1792652484852,"version":"4.5.0"},"reference-count":15,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A new human coronavirus (CoV), subsequently named Middle East respiratory syndrome (MERS)-CoV, was first reported in Saudi Arabia in September 2012. In response, we developed two real-time reverse transcription-PCR (rRT-PCR) assays targeting the MERS-CoV nucleocapsid (N) gene and evaluated these assays as a panel with a previously published assay targeting the region upstream of the MERS-CoV envelope gene (upE) for the detection and confirmation of MERS-CoV infection. All assays detected \u226410 copies\/reaction of quantified RNA transcripts, with a linear dynamic range of 8 log units and 1.3 \u00d7 10\n            <jats:sup>\u22123<\/jats:sup>\n            50% tissue culture infective doses (TCID\n            <jats:sub>50<\/jats:sub>\n            )\/ml of cultured MERS-CoV per reaction. All assays performed comparably with respiratory, serum, and stool specimens spiked with cultured virus. No false-positive amplifications were obtained with other human coronaviruses or common respiratory viral pathogens or with 336 diverse clinical specimens from non-MERS-CoV cases; specimens from two confirmed MERS-CoV cases were positive with all assay signatures. In June 2012, the U.S. Food and Drug Administration authorized emergency use of the rRT-PCR assay panel as an\n            <jats:italic>in vitro<\/jats:italic>\n            diagnostic test for MERS-CoV. A kit consisting of the three assay signatures and a positive control was assembled and distributed to public health laboratories in the United States and internationally to support MERS-CoV surveillance and public health responses.\n          <\/jats:p>","DOI":"10.1128\/jcm.02533-13","type":"journal-article","created":{"date-parts":[[2013,10,24]],"date-time":"2013-10-24T03:09:23Z","timestamp":1382584163000},"page":"67-75","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":145,"title":["Real-Time Reverse Transcription-PCR Assay Panel for Middle East Respiratory Syndrome Coronavirus"],"prefix":"10.1128","volume":"52","author":[{"given":"Xiaoyan","family":"Lu","sequence":"first","affiliation":[{"name":"Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brett","family":"Whitaker","sequence":"additional","affiliation":[{"name":"Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Senthil Kumar K.","family":"Sakthivel","sequence":"additional","affiliation":[{"name":"Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shifaq","family":"Kamili","sequence":"additional","affiliation":[{"name":"Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura E.","family":"Rose","sequence":"additional","affiliation":[{"name":"Division of Preparedness and Emerging Infections, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Lowe","sequence":"additional","affiliation":[{"name":"Division of Preparedness and Emerging Infections, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emad","family":"Mohareb","sequence":"additional","affiliation":[{"name":"U.S. Naval Medical Research Unit 3, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emad M.","family":"Elassal","sequence":"additional","affiliation":[{"name":"U.S. Naval Medical Research Unit 3, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarek","family":"Al-sanouri","sequence":"additional","affiliation":[{"name":"Central Public Health Laboratory, Ministry of Health, Amman, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aktham","family":"Haddadin","sequence":"additional","affiliation":[{"name":"Central Public Health Laboratory, Ministry of Health, Amman, Jordan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dean D.","family":"Erdman","sequence":"additional","affiliation":[{"name":"Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1211721"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00473-12"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01244-13"},{"key":"e_1_3_2_5_2","unstructured":"CormanVMEckerleIBleickerTZakiALandtOEschbach-BludauMvan BoheemenSGopalRBallhauseMBestebroerTMMuthDMullerMADrexlerJFZambonMOsterhausADFouchierRMDrostenC. 2012. Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Euro Surveill. 17:20285. http:\/\/www.eurosurveillance.org\/ViewArticle.aspx?ArticleId=20285."},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","unstructured":"CormanVMMullerMACostabelUTimmJBingerTMeyerBKreherPLattweinEEschbach-BludauMNitscheABleickerTLandtOSchweigerBDrexlerJFOsterhausADHaagmansBLDittmerUBoninFWolffTDrostenC. 2012. Assays for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections. 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Novel human coronavirus not detected in children hospitalized with acute respiratory illness in Amman, Jordan, March 2010\u2013September 2012. Clin. Microbiol. Infect., in press.","journal-title":"Clin. Microbiol. Infect."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02533-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02533-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:49:20Z","timestamp":1646480960000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02533-13"}},"subtitle":[],"editor":[{"given":"A. 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