{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T03:43:52Z","timestamp":1773287032159,"version":"3.50.1"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T00:00:00Z","timestamp":1314835200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A multiple-locus variable-number tandem-repeat analysis (MLVA) was applied to investigate the epidemiological relationship and genetic diversity among 162 human\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            isolates collected from all geographic regions of Turkey in an 8-year period (2001 to 2008). The isolates were genotyped by using an MLVA assay developed in Orsay, France (MLVA-16\n            <jats:sub>Orsay<\/jats:sub>\n            ) including eight minisatellite (panel 1) and eight microsatellite (panel 2, subdivided into 2A and 2B) markers. Panels 1 and 2A distinguish 14 genotypes; two of these represented 85% of the strains. Panel 2B displayed a very high discriminatory power. Three loci from panel 2B had diversity index values higher than 0.74. MLVA-16\n            <jats:sub>Orsay<\/jats:sub>\n            yielded 105 genotypes; 73 were represented by a unique isolate, and 32 included two to eight isolates. The isolates from different patients within the same outbreak or from the same patient before first-line therapy and after relapse showed identical genotypes. A number of MLVA genotypes appeared to be partially restricted to some geographic areas and displayed no annual variation, possibly reflecting persistence of genotypes in certain areas for a time span of at least a decade. This study, representing the first molecular typing results of human\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            isolates from Turkey, indicated that Turkish human\n            <jats:named-content content-type=\"genus-species\">Brucella melitensis<\/jats:named-content>\n            isolates were most closely related to the neighboring countries' isolates included in the East Mediterranean group.\n          <\/jats:p>","DOI":"10.1128\/jcm.02538-10","type":"journal-article","created":{"date-parts":[[2011,7,28]],"date-time":"2011-07-28T04:12:36Z","timestamp":1311826356000},"page":"3276-3283","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["Multiple-Locus Variable-Number Tandem-Repeat Analysis Genotyping of Human Brucella Isolates from Turkey"],"prefix":"10.1128","volume":"49","author":[{"given":"Sel\u00e7uk","family":"K\u0131l\u0131\u00e7","sequence":"first","affiliation":[{"name":"Refik Saydam National Public Health Agency, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivan N.","family":"Ivanov","sequence":"additional","affiliation":[{"name":"National Centre of Infectious and Parasitic Diseases, Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u0131za","family":"Durmaz","sequence":"additional","affiliation":[{"name":"Refik Saydam National Public Health Agency, Ankara, Turkey"},{"name":"Department of Microbiology and Clinical Microbiology, Inonu University, Malatya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehmet Refik","family":"Bayraktar","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Clinical Microbiology, Harran University, Sanliurfa, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ergin","family":"Aya\u015fl\u0131o\u011flu","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Clinical Microbiology, Kirikkale University, Kirikkale, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. Hamidullah","family":"Uyan\u0131k","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Clinical Microbiology, Ataturk University, Erzurum, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hikmet","family":"Al\u0131\u015fkan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Clinical Microbiology, Baskent University, Adana, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ekrem","family":"Ya\u015far","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory, Children's Hospital, Diyarbakir, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G\u00fcl\u00e7in","family":"Bayramo\u011flu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Clinical Microbiology, Karadeniz Technical University, Trabzon, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmet","family":"Arslant\u00fcrk","sequence":"additional","affiliation":[{"name":"Refik Saydam National Public Health Agency, Ankara, Turkey"},{"name":"Microbiology Laboratory, State Hospital, Kutahya, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gilles","family":"Vergnaud","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Sud 11, CNRS, UMR 8621, Institut de G\u00e9n\u00e9tique et Microbiologie, Orsay, France"},{"name":"DGA\/MRIS, Mission pour la Recherche et l'Innovation Scientifique, Bagneux, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Todor V.","family":"Kantardjiev","sequence":"additional","affiliation":[{"name":"National Centre of Infectious and Parasitic Diseases, Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2006.12.015"},{"key":"e_1_3_2_3_2","unstructured":"AltonG. 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World Health Organization, Geneva, Switzerland."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02538-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02538-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:49:07Z","timestamp":1646484547000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02538-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,9]]},"references-count":26,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2011,9]]}},"alternative-id":["10.1128\/JCM.02538-10"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02538-10","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,9]]},"assertion":[{"value":"2010-12-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-04-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-08-30","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}