{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,14]],"date-time":"2026-02-14T11:23:27Z","timestamp":1771068207097,"version":"3.50.1"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T00:00:00Z","timestamp":1149120000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Paracoccidioides brasiliensis<\/jats:italic>\n            is the etiological agent of paracoccidioidomycosis, an important human systemic mycosis in Latin America. Recently, the existence of three different phylogenetic species (S1, PS2, and PS3) of\n            <jats:italic>P. brasiliensis<\/jats:italic>\n            was demonstrated. Despite being genetically isolated, all three species were capable of inducing disease in both humans and animals, although lower virulence has been found with the PS2 species. The available molecular methods developed to characterize and type strains have not been useful for assigning isolates to the described species, creating the need for molecular markers capable of distinguishing genetically isolated groups. Here, we describe a PCR and sequencing-based microsatellite marker system that is stable, easy to assay, adaptable to large series of isolates, and discriminatory enough to be used as a typing system in identifying the three proposed species of\n            <jats:italic>P. brasiliensis<\/jats:italic>\n            . In addition, this system provides an unambiguous tool for strain discrimination between two (S1 and PS2) of the three phylogenetic species.\n          <\/jats:p>","DOI":"10.1128\/jcm.02540-05","type":"journal-article","created":{"date-parts":[[2006,6,7]],"date-time":"2006-06-07T10:58:07Z","timestamp":1149677887000},"page":"2153-2157","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":73,"title":["Microsatellite Analysis of Three Phylogenetic Species of\n            <i>Paracoccidioides brasiliensis<\/i>"],"prefix":"10.1128","volume":"44","author":[{"given":"Daniel R.","family":"Matute","sequence":"first","affiliation":[{"name":"Corporacio\u0301n para Investigaciones Biolo\u0301gicas (CIB), Medelli\u0301n, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria E.","family":"Sepulveda","sequence":"additional","affiliation":[{"name":"Corporacio\u0301n para Investigaciones Biolo\u0301gicas (CIB), Medelli\u0301n, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lina M.","family":"Quesada","sequence":"additional","affiliation":[{"name":"Laboratorio de Micologi\u0301a y Fitopatologi\u0301a Uniandes, Universidad de los Andes, Bogota\u0301, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo H.","family":"Goldman","sequence":"additional","affiliation":[{"name":"Faculdade de Cie\u0302ncias Farmace\u0302uticas de Ribera\u0303o Preto, Universidade de Sa\u0303o Paulo, Sa\u0303o Paulo, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John W.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Department of Plant and Microbial Biology, 111 Koshland Hall, University of California, Berkeley, California 94720-3102"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angela","family":"Restrepo","sequence":"additional","affiliation":[{"name":"Corporacio\u0301n para Investigaciones Biolo\u0301gicas (CIB), Medelli\u0301n, Colombia"},{"name":"Facultad de Medicina, Universidad Pontificia Bolivariana, Medelli\u0301n, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan G.","family":"McEwen","sequence":"additional","affiliation":[{"name":"Corporacio\u0301n para Investigaciones Biolo\u0301gicas (CIB), Medelli\u0301n, Colombia"},{"name":"Facultad de Medicina, Universidad de Antioquia, Medelli\u0301n, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.7.2683-2686.2001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.6.2.89"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2004.09.008"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-311X2002000500037"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.1996.tb00108.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.104.029322"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46651999000600004"},{"key":"e_1_3_2_9_2","first-page":"435","volume":"5","year":"2004","unstructured":"Ellegren, H. 2004. Microsatellites: simple sequences with complex evolution. Nat. Rev. Genet.5:435-445.","journal-title":"Nat. Rev. Genet."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.132178099"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.9.4574-4579.2005"},{"key":"e_1_3_2_12_2","unstructured":"Microsatellites: evolution and applications 1999"},{"key":"e_1_3_2_13_2","first-page":"89","volume":"41","year":"2003","unstructured":"Hebeler-Barbosa, F., M. R. Montenegro, and E. Bagagli. 2003. Virulence profiles of ten Paracoccidioides brasiliensis isolates obtained from armadillos (Dasypus novemcinctus). Med. Mycol.41:89-96.","journal-title":"Med. 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Pappas, and J. D. Sobel (ed.), Clinical mycology. Oxford University Press, Oxford, United Kingdom.","journal-title":"Clinical mycology"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(98)70989-3"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-294X.2003.01825.x"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5274(03)00088-2"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-280X.1998.00161.x"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02540-05","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02540-05","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T05:15:29Z","timestamp":1646457329000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02540-05"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,6]]},"references-count":26,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,6]]}},"alternative-id":["10.1128\/JCM.02540-05"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02540-05","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,6]]},"assertion":[{"value":"2005-12-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-03-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}