{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T02:16:26Z","timestamp":1784081786458,"version":"3.55.0"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A large-scale, whole-genome comparison of Canadian\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n            isolates with high-level cephalosporin MICs was used to demonstrate a genomic epidemiology approach to investigate strain relatedness and dynamics. Although current typing methods have been very successful in tracing short-chain transmission of gonorrheal disease, investigating the temporal evolutionary relationships and geographical dissemination of highly clonal lineages requires enhanced resolution only available through whole-genome sequencing (WGS). Phylogenomic cluster analysis grouped 169 Canadian strains into 12 distinct clades. While some\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            multiantigen sequence types (NG-MAST) agreed with specific phylogenomic clades or subclades, other sequence types (ST) and closely related groups of ST were widely distributed among clades. Decreased susceptibility to extended-spectrum cephalosporins (ESC-DS) emerged among a group of diverse strains in Canada during the 1990s with a variety of nonmosaic\n            <jats:italic>penA<\/jats:italic>\n            alleles, followed in 2000\/2001 with the\n            <jats:italic>penA<\/jats:italic>\n            mosaic X allele and then in 2007 with ST1407 strains with the\n            <jats:italic>penA<\/jats:italic>\n            mosaic XXXIV allele. Five genetically distinct ESC-DS lineages were associated with\n            <jats:italic>penA<\/jats:italic>\n            mosaic X, XXXV, and XXXIV alleles and nonmosaic XII and XIII alleles. ESC-DS with coresistance to azithromycin was observed in 5 strains with 23S rRNA C2599T or A2143G mutations. As the costs associated with WGS decline and analysis tools are streamlined, WGS can provide a more thorough understanding of strain dynamics, facilitate epidemiological studies to better resolve social networks, and improve surveillance to optimize treatment for gonorrheal infections.\n          <\/jats:p>","DOI":"10.1128\/jcm.02589-14","type":"journal-article","created":{"date-parts":[[2014,11,7]],"date-time":"2014-11-07T08:20:55Z","timestamp":1415348455000},"page":"191-200","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":104,"title":["Whole-Genome Phylogenomic Heterogeneity of Neisseria gonorrhoeae Isolates with Decreased Cephalosporin Susceptibility Collected in Canada between 1989 and 2013"],"prefix":"10.1128","volume":"53","author":[{"given":"Walter","family":"Demczuk","sequence":"first","affiliation":[{"name":"Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarah","family":"Lynch","sequence":"additional","affiliation":[{"name":"Alberta Health Services, Calgary, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Martin","sequence":"additional","affiliation":[{"name":"Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary","family":"Van Domselaar","sequence":"additional","affiliation":[{"name":"Science Technology Cores and Services Division, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada, and Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morag","family":"Graham","sequence":"additional","affiliation":[{"name":"Science Technology Cores and Services Division, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada, and Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amrita","family":"Bharat","sequence":"additional","affiliation":[{"name":"Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanessa","family":"Allen","sequence":"additional","affiliation":[{"name":"Public Health Ontario Laboratories, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Hoang","sequence":"additional","affiliation":[{"name":"British Columbia Public Health Microbiology and Reference Laboratory, British Columbia Centre for Disease Control, Vancouver, British Columbia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"Lefebvre","sequence":"additional","affiliation":[{"name":"Laboratoire de Sant\u00e9 Publique du Qu\u00e9bec, Sainte-Anne-de-Bellevue, Qu\u00e9bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greg","family":"Tyrrell","sequence":"additional","affiliation":[{"name":"Provincial Laboratory for Public Health, Edmonton, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Greg","family":"Horsman","sequence":"additional","affiliation":[{"name":"Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Haldane","sequence":"additional","affiliation":[{"name":"Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Garceau","sequence":"additional","affiliation":[{"name":"Dr. G.L. Dumont Hospital, Moncton, New Brunswick, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Wylie","sequence":"additional","affiliation":[{"name":"Cadham Provincial Laboratory, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom","family":"Wong","sequence":"additional","affiliation":[{"name":"Centre for Communicable Diseases and Infection Control, Public Health Agency of Canada, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Mulvey","sequence":"additional","affiliation":[{"name":"Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"Notifiable diseases on-line","author":"Public Health Agency of Canada","year":"2014","unstructured":"Public Health Agency of Canada. 2014. Notifiable diseases on-line. Public Health Agency of Canada, Ottawa, Ontario, Canada. Accessed 4 September 2014. http:\/\/dsol-smed.phac-aspc.gc.ca\/dsol-smed\/ndis\/charts.php?c=pl."},{"key":"e_1_3_3_3_2","volume-title":"Global action plan to control the spread and impact of antimicrobial resistance in Neisseria gonorrhoeae","author":"World Health Organization","year":"2012","unstructured":"World Health Organization. 2012. Global action plan to control the spread and impact of antimicrobial resistance in Neisseria gonorrhoeae. World Health Organization, Geneva, Switzerland. http:\/\/www.who.int\/reproductivehealth\/publications\/rtis\/9789241503501\/en\/. Accessed 12 June 2012."},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1517\/14656560902731993"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00010-14"},{"key":"e_1_3_3_6_2","volume-title":"Canadian guidelines on sexually transmitted infections, gonococcal infections. Revised July 2013","author":"Public Health Agency of Canada","year":"2013","unstructured":"Public Health Agency of Canada. 2013. Canadian guidelines on sexually transmitted infections, gonococcal infections. Revised July 2013. Community Acquired Infections Division, Centre for Communicable Diseases and Infection Control, Public Health Agency of Canada, Ottawa, Ontario, Canada. Accessed 22 July 2014. http:\/\/www.phac-aspc.gc.ca\/std-mts\/sti-its\/index-eng.php."},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3182401b69"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2012.176575"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0000000000000033"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00325-11"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05760-11"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.2807\/ese.15.47.19721-en"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.2807\/ese.16.14.19833-en"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.2807\/ese.16.43.19998-en"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02608-13"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01604-06"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp505"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-008-0610-7"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00306-07"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1586\/eri.09.63"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00304-09"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.9.2811-2820.2002"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.7.2300-2308.2006"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2012.08.005"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2010.045377"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.3.769-777.2002"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq377"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04752.x"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.9.3020-3025.2002"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.43.10.2468"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/47.5.651"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.1.1-12.2001"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00040-10"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1086\/383047"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1159\/000342709"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(13)70693-5"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(13)70700-X"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31822c664f"},{"key":"e_1_3_3_39_2","volume-title":"Twenty-fourth informational supplement M100-S24","author":"Clinical and Laboratory Standards Institute","year":"2014","unstructured":"Clinical and Laboratory Standards Institute. 2014. Performance standards for antimicrobial susceptibility testing. Twenty-fourth informational supplement M100-S24. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199607000-00004"},{"key":"e_1_3_3_41_2","volume-title":"Sexually transmitted disease surveillance 2007 supplement. Gonococcal Isolate Surveillance Project (GISP) annual report 2007","author":"Centers for Disease Control and Prevention","year":"2009","unstructured":"Centers for Disease Control and Prevention. 2009. Sexually transmitted disease surveillance 2007 supplement. Gonococcal Isolate Surveillance Project (GISP) annual report 2007. Division of STD Prevention, National Center for HIV\/AIDS, Viral Hepatitis, STD, and TB Prevention, Centers for Disease Control and Prevention, Atlanta, GA. Accessed 30 November 2010. http:\/\/www.cdc.gov\/std\/gisp2007\/gispsurvsupp2007short.pdf."},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp098"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.4103\/0301-4738.37595"},{"key":"e_1_3_3_44_2","unstructured":"Dean AG Sullivan KM Soe MM. 2013. OpenEpi: open source epidemiologic statistics for public health version 3.01. www.openepi.com. Accessed 8 August 2014."},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr507"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq284"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/syq010"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btm404"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00626-06"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.12.117"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00406-09"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01295-13"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.2807\/ese.18.03.20358-en"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01502-14"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.12.3603-3606.2001"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.12.3744-3749.2002"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.1.137-143.2005"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.05481-11"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01010-09"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02589-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02589-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:14:44Z","timestamp":1646482484000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02589-14"}},"subtitle":[],"editor":[{"given":"E.","family":"Munson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,1]]},"references-count":61,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2015,1]]}},"alternative-id":["10.1128\/JCM.02589-14"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02589-14","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,1]]},"assertion":[{"value":"2014-09-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-10-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-12-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}