{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T13:21:32Z","timestamp":1783948892596,"version":"3.55.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T00:00:00Z","timestamp":1618876800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2021,4,20]],"date-time":"2021-04-20T00:00:00Z","timestamp":1618876800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2021,4,20]]},"abstract":"<jats:p>Serological assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are needed to support clinical diagnosis and epidemiological investigations. Recently, assays for large-scale detection of total antibodies (Ab), immunoglobulin G (IgG), and IgM against SARS-CoV-2 antigens have been developed, but there are limited data on the diagnostic accuracy of these assays.<\/jats:p>","DOI":"10.1128\/jcm.02596-20","type":"journal-article","created":{"date-parts":[[2021,2,12]],"date-time":"2021-02-12T07:58:30Z","timestamp":1613116710000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":109,"title":["Comparison of 16 Serological SARS-CoV-2 Immunoassays in 16 Clinical Laboratories"],"prefix":"10.1128","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3100-6256","authenticated-orcid":true,"given":"Lene H.","family":"Harritsh\u00f8j","sequence":"first","affiliation":[{"name":"Department of Clinical Immunology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5716-5899","authenticated-orcid":true,"given":"Mikkel","family":"Gybel-Brask","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoaib","family":"Afzal","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Copenhagen University Hospital, Herlev and Gentofte Hospital, Herlev, Denmark"},{"name":"Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pia R.","family":"Kamstrup","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Copenhagen University Hospital, Herlev and Gentofte Hospital, Herlev, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte S.","family":"J\u00f8rgensen","sequence":"additional","affiliation":[{"name":"Department of Virus &amp; Microbiological Special Diagnostics, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne Kragh","family":"Thomsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda","family":"Hilsted","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lennart","family":"Friis-Hansen","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Copenhagen University Hospital, Bispebjerg, and Frederiksberg Hospital, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pal B.","family":"Szecsi","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Holb\u00e6k Hospital, Holb\u00e6k, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lise","family":"Pedersen","sequence":"additional","affiliation":[{"name":"Department of Clinical Biochemistry, Holb\u00e6k Hospital, Holb\u00e6k, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lene","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital, Herlev and Gentofte Hospital, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilie B.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Medicine, Department of Clinical Immunology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Garred","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Medicine, Department of Clinical Immunology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"},{"name":"Department of Clinical Medicine, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trine-Line","family":"Korsholm","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan","family":"Mikkelsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirstine O.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bjarne K.","family":"M\u00f8ller","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Aarhus University Hospital, Aarhus, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne T.","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kasper K.","family":"Iversen","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Herlev og Gentofte Hospital, University of Copenhagen, Herlev, Denmark"},{"name":"Department of Emergency Medicine, Herlev og Gentofte Hospital, University of Copenhagen, Herlev, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pernille B.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Herlev og Gentofte Hospital, University of Copenhagen, Herlev, Denmark"},{"name":"Department of Emergency Medicine, Herlev og Gentofte Hospital, University of Copenhagen, Herlev, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasmus B.","family":"Hasselbalch","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Herlev og Gentofte Hospital, University of Copenhagen, Herlev, Denmark"},{"name":"Department of Emergency Medicine, Herlev og Gentofte Hospital, University of Copenhagen, Herlev, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamille","family":"Fogh","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Herlev og Gentofte Hospital, University of Copenhagen, Herlev, Denmark"},{"name":"Department of Emergency Medicine, Herlev og Gentofte Hospital, University of Copenhagen, Herlev, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob B.","family":"Norsk","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Herlev og Gentofte Hospital, University of Copenhagen, Herlev, Denmark"},{"name":"Department of Emergency Medicine, Herlev og Gentofte Hospital, University of Copenhagen, Herlev, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas Henrik","family":"Kristensen","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Herlev og Gentofte Hospital, University of Copenhagen, Herlev, Denmark"},{"name":"Department of Emergency Medicine, Herlev og Gentofte Hospital, University of Copenhagen, Herlev, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7767-305X","authenticated-orcid":true,"given":"Kristian","family":"Sch\u00f8nning","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nikolai S.","family":"Kirkby","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex C. Y.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lone H.","family":"Landsy","sequence":"additional","affiliation":[{"name":"Department of Nonclinical and Clinical Assay Sciences in Global Discovery &amp; Development Sciences, Novo Nordisk A\/S, M\u00e5l\u00f8v, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mette","family":"Loftager","sequence":"additional","affiliation":[{"name":"Department of Nonclinical and Clinical Assay Sciences in Global Discovery &amp; Development Sciences, Novo Nordisk A\/S, M\u00e5l\u00f8v, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorte K.","family":"Holm","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Odense University Hospital, Odense, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna C.","family":"Nilsson","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Odense University Hospital, Odense, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne G.","family":"S\u00e6kmose","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Zealand University Hospital, N\u00e6stved Hospital, N\u00e6stved, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgitte","family":"Grum-Schwensen","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Zealand University Hospital, N\u00e6stved Hospital, N\u00e6stved, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bitten","family":"Aagaard","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Aalborg University Hospital, Aalborg, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Th\u00f8ger G.","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Odense University Hospital, Odense, Denmark"},{"name":"Research Unit for Clinical Microbiology, Odense University Hospital, University of Southern Denmark, Odense, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorte M.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Zealand University Hospital, Slagelse Hospital, Slagelse, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Ullum","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark"},{"name":"Department of Clinical Medicine, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark"},{"name":"Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1497-6309","authenticated-orcid":true,"given":"Ram B.","family":"Dessau","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Zealand University Hospital, Slagelse Hospital, Slagelse, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","unstructured":"Ghebreyesus T. 2020. WHO Director-General's opening remarks at the media briefing on COVID-19\u201411 March 2020."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9030231"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.12932\/AP-200220-0772"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD013652"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00797-20"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-020-0659-0"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.m2516"},{"key":"e_1_3_3_9_2","article-title":"Evaluation of nine commercial SARS-CoV-2 immunoassays","author":"Ria Lassauni\u00e8re AF","year":"2020","unstructured":"Ria Lassauni\u00e8re AF, Harboe ZB, Nielsen AC, Fomsgaard A, Krogfelt KA, J\u00f8rgensen CS. 2020. Evaluation of nine commercial SARS-CoV-2 immunoassays. medRxiv. https:\/\/www.medrxiv.org\/content\/10.1101\/2020.04.09.20056325v1.","journal-title":"medRxiv"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2020.104480"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.es2014.19.1.20667"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2000898"},{"key":"e_1_3_3_13_2","unstructured":"R Core Team. 2020. R: a language and environment for statistical computing. R Foundation for Statistical Computing Vienna Austria. https:\/\/www.R-project.org\/."},{"key":"e_1_3_3_14_2","unstructured":"Doebbler P. 2020. mada: meta-analysis of diagnostic accuracy. CRAN.R-project.org\/package=mada."},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.m2655"},{"key":"e_1_3_3_16_2","unstructured":"Medicines and Healthcare Products Regulatory Agency. 2020. Target product profile: enzyme immunoassay (EIA) antibody tests to help determine if people have antibodies to SARS-CoV-2. https:\/\/www.gov.uk\/government\/publications\/how-tests-and-testing-kits-for-coronavirus-covid-19-work\/target-product-profile-enzyme-immunoassay-eia-antibody-tests-to-help-determine-if-people-have-antibodies-to-sars-cov-2."},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2020.25.11.2000266"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa849"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1101\/2020.04.26.20079244:2020.04.26.20079244"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/aqaa140"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2020.06.019"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(20)30634-4"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0897-1"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa344"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00763-2020"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18450-4"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa489"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-020-00813-8"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/hvaa137"},{"key":"e_1_3_3_30_2","article-title":"Longitudinal evaluation and decline of antibody responses in SARS-CoV-2 infection","author":"Seow JGC","year":"2020","unstructured":"Seow JGC, Merrick B, Acors S, Steel KJA, Hemmings O, O'Bryne A, Kouphou N, Pickering S, Galao R, Betancor G, Wilson HD, Signell AW, Winstone H, Kerridge C, Temperton N, Snell L, Bisnauthsing K, Moore A, Green A, Martinez L, Stokes B, Honey J, Izquierdo-Barras A, Arbane G, Patel A, OConnell L, O'Hara G, MacMahon E, Douthwaite S, Nebbia S, Batra R, Martinez-Nunez R, Edgeworth JD, Neil SJD, Malim M, Doores K. 2020. Longitudinal evaluation and decline of antibody responses in SARS-CoV-2 infection. medRxiv. https:\/\/doi.org\/10.1101\/2020.07.09.20148429.","journal-title":"medRxiv"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(20)30453-7"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-17317-y"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02596-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02596-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T05:58:16Z","timestamp":1646459896000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02596-20"}},"subtitle":[],"editor":[{"given":"Yi-Wei","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,4,20]]},"references-count":31,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2021,4,20]]}},"alternative-id":["10.1128\/JCM.02596-20"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02596-20","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.07.30.20165373","asserted-by":"object"}]},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,20]]},"assertion":[{"value":"2020-10-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-02-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-20","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02596-20"}}