{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:59:40Z","timestamp":1762271980990,"version":"3.37.3"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2016,3,25]],"date-time":"2016-03-25T00:00:00Z","timestamp":1458864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP49932","OPP1084251"],"award-info":[{"award-number":["OPP49932","OPP1084251"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Infection with\n            <jats:named-content content-type=\"genus-species\">Leishmania donovani<\/jats:named-content>\n            is typically asymptomatic, but a significant number of individuals may progress to visceral leishmaniasis (VL), a deadly disease that threatens 200 million people in areas where it is endemic. While diagnosis of acute VL has been simplified by the use of cost-effective confirmatory serological tests, similar standardized tools are not widely available for detecting asymptomatic infection, which can be 4 to 20 times more prevalent than active disease. A simple and accurate serological test that is capable of detecting asymptomatic\n            <jats:named-content content-type=\"genus-species\">L. donovani<\/jats:named-content>\n            infection will be useful for surveillance programs targeting VL control and elimination. To address this unmet need, we evaluated recombinant antigens for their ability to detect serum antibodies in 104 asymptomatic\n            <jats:named-content content-type=\"genus-species\">L. donovani<\/jats:named-content>\n            -infected individuals (qualified as positive for\n            <jats:named-content content-type=\"genus-species\">L. donovani<\/jats:named-content>\n            -specific antibodies by direct agglutination test [DAT]) from the Mymensingh district of Bangladesh where VL is hyperendemic. The novel proteins rKR95 and rTR18 possessed the greatest potential and detected 69% of DAT-positive individuals, with rKR95 being more robust in reactivity. Agreement in results for individuals with high DAT responses, who are more likely to progress to VL disease, was 74%. When considered along with rK39, a gold standard antigen that is used to confirm clinical diagnosis of VL but that is now becoming widely used for surveillance, rKR95 and rTR18 conferred a sensitivity of 84% based on a theoretical combined estimate. Our data indicate that incorporating rKR95 and rTR18 with rK39 in serological tests amenable to rapid or high-throughput screening may enable simple and accurate detection of asymptomatic infection. Such tests will be important tools to measure\n            <jats:named-content content-type=\"genus-species\">L. donovani<\/jats:named-content>\n            infection rates, a primary goal in surveillance and a critical measurement with which to assess elimination programs.\n          <\/jats:p>","DOI":"10.1128\/jcm.02620-15","type":"journal-article","created":{"date-parts":[[2016,2,4]],"date-time":"2016-02-04T03:39:03Z","timestamp":1454557143000},"page":"1025-1030","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Accurate Serodetection of Asymptomatic Leishmania donovani Infection by Use of Defined Antigens"],"prefix":"10.1128","volume":"54","author":[{"given":"Aarthy C.","family":"Vallur","sequence":"first","affiliation":[{"name":"Infectious Disease Research Institute, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Reinhart","sequence":"additional","affiliation":[{"name":"Infectious Disease Research Institute, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raodoh","family":"Mohamath","sequence":"additional","affiliation":[{"name":"Infectious Disease Research Institute, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuyuki","family":"Goto","sequence":"additional","affiliation":[{"name":"Infectious Disease Research Institute, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prakash","family":"Ghosh","sequence":"additional","affiliation":[{"name":"International Center for Diarrhoeal Diseases Research, Laboratory Sciences Division, Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dinesh","family":"Mondal","sequence":"additional","affiliation":[{"name":"International Center for Diarrhoeal Diseases Research, Laboratory Sciences Division, Dhaka, Bangladesh"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malcolm S.","family":"Duthie","sequence":"additional","affiliation":[{"name":"Infectious Disease Research Institute, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven G.","family":"Reed","sequence":"additional","affiliation":[{"name":"Infectious Disease Research Institute, Seattle, Washington, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035671"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2004.03.004"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000599"},{"key":"e_1_3_2_5_2","first-page":"105","article-title":"Can visceral leishmaniasis be eliminated from Asia?","volume":"45","author":"Joshi A","year":"2008","unstructured":"Joshi A, Narain JP, Prasittisuk C, Bhatia R, Hashim G, Jorge A, Banjara M, Kroeger A. 2008. 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