{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T15:53:32Z","timestamp":1765382012263,"version":"3.37.3"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2016,3,1]],"date-time":"2016-03-01T00:00:00Z","timestamp":1456790400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100004334","name":"Merck","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004334","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Significant interlaboratory variability is observed in testing the caspofungin susceptibility of\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            species by both the CLSI and EUCAST broth microdilution methodologies. We evaluated the influence of treated versus untreated polystyrene microtiter trays on caspofungin MICs using 209 isolates of four\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            species, including 16\n            <jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>\n            and 11\n            <jats:named-content content-type=\"genus-species\">C. glabrata<\/jats:named-content>\n            isolates with defined\n            <jats:italic>FKS<\/jats:italic>\n            mutations. Caspofungin MICs were also determined using the commercially available YeastOne and Etest assays and 102 isolates. All\n            <jats:named-content content-type=\"genus-species\">C. glabrata<\/jats:named-content>\n            isolates had caspofungin MICs of \u22650.5 \u03bcg\/ml, the clinical breakpoint for caspofungin resistance in this species, measured using trays made of treated polystyrene, regardless of the\n            <jats:italic>FKS<\/jats:italic>\n            status. In contrast, susceptible isolates could readily be distinguished from resistant\/non-wild-type isolates when caspofungin MICs were measured using untreated polystyrene trays and both the YeastOne and Etest assays. Similar results were also observed for\n            <jats:named-content content-type=\"genus-species\">C. krusei<\/jats:named-content>\n            isolates, as all isolates had caspofungin MICs above the threshold for resistance measured using treated polystyrene trays. In contrast,\n            <jats:named-content content-type=\"genus-species\">C. albicans<\/jats:named-content>\n            isolates could be correctly identified as susceptible or resistant when caspofungin MICs were measured with treated or untreated trays and with the YeastOne and Etest assays. MICs falsely elevated above the resistance breakpoint were also not observed for\n            <jats:named-content content-type=\"genus-species\">C. tropicalis<\/jats:named-content>\n            isolates. These results demonstrated that the use of treated polystyrene may be one factor that leads to falsely elevated caspofungin\n            <jats:italic>in vitro<\/jats:italic>\n            susceptibility results and that this may also be a greater issue for some\n            <jats:named-content content-type=\"genus-species\">Candida<\/jats:named-content>\n            species than for others.\n          <\/jats:p>","DOI":"10.1128\/jcm.02659-15","type":"journal-article","created":{"date-parts":[[2016,1,14]],"date-time":"2016-01-14T09:57:12Z","timestamp":1452765432000},"page":"734-738","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Effects of Treated versus Untreated Polystyrene on Caspofungin\n            <i>In Vitro<\/i>\n            Activity against Candida Species"],"prefix":"10.1128","volume":"54","author":[{"given":"Annette W.","family":"Fothergill","sequence":"first","affiliation":[{"name":"Fungus Testing Laboratory, Department of Pathology and South Texas Reference Laboratories, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dora I.","family":"McCarthy","sequence":"additional","affiliation":[{"name":"Fungus Testing Laboratory, Department of Pathology and South Texas Reference Laboratories, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad T.","family":"Albataineh","sequence":"additional","affiliation":[{"name":"Fungus Testing Laboratory, Department of Pathology and South Texas Reference Laboratories, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmita","family":"Sanders","sequence":"additional","affiliation":[{"name":"Fungus Testing Laboratory, Department of Pathology and South Texas Reference Laboratories, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"McElmeel","sequence":"additional","affiliation":[{"name":"Fungus Testing Laboratory, Department of Pathology and South Texas Reference Laboratories, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan P.","family":"Wiederhold","sequence":"additional","affiliation":[{"name":"Fungus Testing Laboratory, Department of Pathology and South Texas Reference Laboratories, University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01519-13"},{"key":"e_1_3_2_3_2","volume-title":"The impact of new CLSI breakpoints on antifungal resistance in Candida species","author":"Fothergill AW","year":"2013","unstructured":"Fothergill AW, Sutton DA, McCarthy DI, Wiederhold NP. 2013. The impact of new CLSI breakpoints on antifungal resistance in Candida species, abstr M-1364. Abstr Intersci Conf Antimicrob Agents Chemother, Denver, CO."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00027-12"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00959-08"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00443-09"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01568-07"},{"key":"e_1_3_2_8_2","volume-title":"CLSI document M27-A3","author":"CLSI","year":"2008","unstructured":"CLSI. 2008. Reference method for broth dilution antifungal susceptibility testing of yeasts; approved standard\u20143rd ed. CLSI document M27-A3. Clinical and Laboratory Standards Institute, Wayne, PA.","edition":"3"},{"key":"e_1_3_2_9_2","volume-title":"CLSI document M27-S4","author":"CLSI","year":"2012","unstructured":"CLSI. 2012. Reference method for broth dilution antifungal susceptibility testing of yeasts; fourth informational supplement. CLSI document M27-S4. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01250-15"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02481-13"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00782-14"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02163-13"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03385-12"},{"key":"e_1_3_2_15_2","volume-title":"Colistin is extensively lost during normal experimental conditions","author":"Karvanen M","year":"2011","unstructured":"Karvanen M, Malmber C, Mohamed A, Lagerback P, Friberg LE, Cars. 2011. Colistin is extensively lost during normal experimental conditions, abstr D-690. Abstr Intersci Conf Antimicrob Agents Chemother, Washington, DC."},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt503"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/phar.1505"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.08.025"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02659-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02659-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T08:49:28Z","timestamp":1646470168000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02659-15"}},"subtitle":[],"editor":[{"given":"D. W.","family":"Warnock","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2016,3]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2016,3]]}},"alternative-id":["10.1128\/JCM.02659-15"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02659-15","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"type":"print","value":"0095-1137"},{"type":"electronic","value":"1098-660X"}],"subject":[],"published":{"date-parts":[[2016,3]]},"assertion":[{"value":"2015-09-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-01-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-02-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}