{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:59:45Z","timestamp":1791356385461,"version":"4.3.1"},"reference-count":33,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T00:00:00Z","timestamp":1359676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A method for rapid species identification of ticks may help clinicians predict the disease outcomes of patients with tick bites and may inform the decision as to whether to administer postexposure prophylactic antibiotic treatment. We aimed to establish a matrix-assisted laser desorption ionization\u2013time of flight mass spectrometry (MALDI-TOF MS) spectrum database based on the analysis of the legs of six tick vectors:\n            <jats:named-content content-type=\"genus-species\">Amblyomma variegatum<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Rhipicephalus sanguineus<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Hyalomma marginatum rufipes<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Ixodes ricinus<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Dermacentor marginatus<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Dermacentor reticulatus<\/jats:named-content>\n            . A blind test was performed on a trial set of ticks to identify specimens of each species. Subsequently, we used MALDI-TOF MS to identify ticks obtained from the wild or removed from patients. The latter tick samples were also identified by 12S ribosomal DNA (rDNA) sequencing and were tested for bacterial infections. Ticks obtained from the wild or removed from patients (\n            <jats:named-content content-type=\"genus-species\">R. sanguineus<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">I. ricinus<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">D. marginatus<\/jats:named-content>\n            ) were accurately identified using MALDI-TOF MS, with the exception of those ticks for which no spectra were available in the database. Furthermore, one damaged specimen was correctly identified as\n            <jats:named-content content-type=\"genus-species\">I. ricinus<\/jats:named-content>\n            , a vector of Lyme disease, using MALDI-TOF MS only. Six of the 14 ticks removed from patients were found to be infected by pathogens that included\n            <jats:named-content content-type=\"genus-species\">Rickettsia<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Anaplasma<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Borrelia<\/jats:named-content>\n            spp. MALDI-TOF MS appears to be an effective tool for the rapid identification of tick vectors that requires no previous expertise in tick identification. The benefits for clinicians include the more targeted surveillance of patients for symptoms of potentially transmitted diseases and the ability to make more informed decisions as to whether to administer postexposure prophylactic treatment.\n          <\/jats:p>","DOI":"10.1128\/jcm.02665-12","type":"journal-article","created":{"date-parts":[[2012,12,6]],"date-time":"2012-12-06T05:42:36Z","timestamp":1354772556000},"page":"522-528","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":146,"title":["Matrix-Assisted Laser Desorption Ionization\u2013Time of Flight Mass Spectrometry for Rapid Identification of Tick Vectors"],"prefix":"10.1128","volume":"51","author":[{"given":"Amina","family":"Yssouf","sequence":"first","affiliation":[{"name":"Aix Marseille Universit\u00e9, Unit\u00e9 de Recherche en Maladies Infectieuses et Tropicales Emergentes, UM63, CNRS 7278, IRD 198, Inserm 1095, WHO Collaborative Center for Rickettsioses and Other Arthropod Borne Bacterial Diseases, Facult\u00e9 de M\u00e9decine, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Flaudrops","sequence":"additional","affiliation":[{"name":"Assistance Publique des H\u00f4pitaux de Marseille, CHU Timone, P\u00f4le Infectieux, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rezak","family":"Drali","sequence":"additional","affiliation":[{"name":"Aix Marseille Universit\u00e9, Unit\u00e9 de Recherche en Maladies Infectieuses et Tropicales Emergentes, UM63, CNRS 7278, IRD 198, Inserm 1095, WHO Collaborative Center for Rickettsioses and Other Arthropod Borne Bacterial Diseases, Facult\u00e9 de M\u00e9decine, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tahar","family":"Kernif","sequence":"additional","affiliation":[{"name":"Aix Marseille Universit\u00e9, Unit\u00e9 de Recherche en Maladies Infectieuses et 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