{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:17:33Z","timestamp":1761059853252},"reference-count":22,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2015,3,1]],"date-time":"2015-03-01T00:00:00Z","timestamp":1425168000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A yet-undescribed bacterial species, tentatively named \u201c\n            <jats:named-content content-type=\"genus-species\">Porphyromonas<\/jats:named-content>\n            <jats:named-content content-type=\"genus-species\">katsikii<\/jats:named-content>\n            ,\u201d was isolated from individuals of a small goat herd with pyogranulomatous pneumonia during an outbreak of acute respiratory disease. The isolated bacteria grew in the form of black-pigmented colonies after 14 days of incubation under anaerobic conditions at 37\u00b0C on a tryptic soy blood agar medium. The bacteria were identified as a yet-undescribed\n            <jats:named-content content-type=\"genus-species\">Porphyromonas<\/jats:named-content>\n            species by determination of the nucleotide sequence of the\n            <jats:italic>rrs<\/jats:italic>\n            16S rRNA gene, and this species was tentatively named\n            <jats:named-content content-type=\"genus-species\">Porphyromonas<\/jats:named-content>\n            <jats:named-content content-type=\"genus-species\">katsikii<\/jats:named-content>\n            . PCR amplification with specific primers for this yet-undescribed species revealed the presence of\n            <jats:named-content content-type=\"genus-species\">P. katsikii<\/jats:named-content>\n            in the lung tissue of all affected animals, while no PCR signals were evidenced from the lungs of healthy goats or from goats with pasteurellosis caused by\n            <jats:named-content content-type=\"genus-species\">Mannheimia haemolytica<\/jats:named-content>\n            . These data indicate\n            <jats:named-content content-type=\"genus-species\">P. katsikii<\/jats:named-content>\n            as the causative agent of acute respiratory distress.\n            <jats:named-content content-type=\"genus-species\">P. katsikii<\/jats:named-content>\n            is phylogenetically related to\n            <jats:named-content content-type=\"genus-species\">Porphyromonas<\/jats:named-content>\n            <jats:named-content content-type=\"genus-species\">somerae<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Porphyromonas levii<\/jats:named-content>\n            , which cause pathologies in humans and animals, respectively.\n            <jats:named-content content-type=\"genus-species\">P. katsikii<\/jats:named-content>\n            was not detected by PCR from samples of the gingival pockets or of the faces of healthy goats.\n          <\/jats:p>","DOI":"10.1128\/jcm.02682-14","type":"journal-article","created":{"date-parts":[[2014,12,25]],"date-time":"2014-12-25T05:59:08Z","timestamp":1419487148000},"page":"795-798","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Pyogranulomatous Pneumonia in Goats Caused by an Undescribed Porphyromonas Species, \u201cPorphyromonas katsikii\u201d"],"prefix":"10.1128","volume":"53","author":[{"given":"George","family":"Filioussis","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology and Infectious Diseases, School of Veterinary Medicine, Aristotle University of Thessaloniki, University Campus, Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evanthia","family":"Petridou","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology and Infectious Diseases, School of Veterinary Medicine, Aristotle University of Thessaloniki, University Campus, Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanouel","family":"Karavanis","sequence":"additional","affiliation":[{"name":"3rd Veterinary Hospital of Greek Army, Thessaloniki, Greece"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joachim","family":"Frey","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Bacteriology, University of Bern, Bern, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(99)00164-8"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/516237"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12067"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/476068"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2011.03.001"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1003.020592"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.9.4455-4459.2005"},{"key":"e_1_3_2_9_2","first-page":"E1","article-title":"Identification of Porphyromonas levii isolated from clinical cases of bovine interdigital necrobacillosis by 16S rRNA sequencing","volume":"10","author":"Sweeney M","year":"2009","unstructured":"Sweeney M, Watts J, Portis E, Lucas M, Nutsch R, Meeuwse D, Bade D, Oliver V, Morck DW, Shinabarger D, Poppe S, Peterson M, Sweeney D, Knechtel M, Zurenko G. 2009. 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