{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T07:55:54Z","timestamp":1789631754381,"version":"build-2803163510"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2015,1,1]],"date-time":"2015-01-01T00:00:00Z","timestamp":1420070400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Immunoprecipitin detection (IPD) is the current reference confirmatory technique for anti-\n                    <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n                    antibody detection; however, the lack of standardization is a critical drawback of this assay. In this study, we evaluated the performance of the\n                    <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n                    Western blot (\n                    <jats:italic>Asp<\/jats:italic>\n                    -WB) IgG kit (LDBio Diagnostics, Lyon, France), a recently commercialized immunoblot assay for the diagnosis of various clinical presentations of chronic aspergillosis. Three hundred eight serum samples from 158 patients with aspergillosis\n                    <jats:named-content content-type=\"genus-species\">sensu lato<\/jats:named-content>\n                    (s.l.) were analyzed. More specifically, 267 serum samples were derived from patients with\n                    <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n                    disease, including 89 cases of chronic pulmonary aspergillosis, 10 of aspergilloma, and 32 of allergic bronchopulmonary aspergillosis, while 41 samples were from patients with\n                    <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n                    colonization, including 15 cystic fibrosis (CF) and 12 non-CF patients. For blood donor controls, the\n                    <jats:italic>Asp<\/jats:italic>\n                    -WB specificity was 94%, while the kit displayed a sensitivity for the aspergillosis s.l. diagnosis of 88.6%, with a diagnostic odds ratio (DOR) of 119 (95% confidence interval [CI], 57 to 251). The DOR values were 185.22 (95% CI,78.79 to 435.45) and 43.74 (95% CI, 15.65 to 122.20) for the diagnosis of\n                    <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n                    disease and\n                    <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n                    colonization, respectively. Among the patients, the sensitivities of the\n                    <jats:italic>Asp<\/jats:italic>\n                    -WB in the diagnosis of\n                    <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n                    colonization were 100% and 41.7% in CF and non-CF patients, respectively. The\n                    <jats:italic>Asp<\/jats:italic>\n                    -WB yielded fewer false-negative results than did IPD. In conclusion, the\n                    <jats:italic>Asp<\/jats:italic>\n                    -WB kit performed well for the diagnosis of various clinical presentations of aspergillosis in nonimmunocompromised patients, with an enhanced standardization and a higher sensitivity than with IPD, which is the current reference method.\n                  <\/jats:p>","DOI":"10.1128\/jcm.02690-14","type":"journal-article","created":{"date-parts":[[2014,11,13]],"date-time":"2014-11-13T02:26:38Z","timestamp":1415845598000},"page":"248-254","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Evaluation of the Aspergillus Western Blot IgG Kit for Diagnosis of Chronic Aspergillosis"],"prefix":"10.1128","volume":"53","author":[{"given":"A.","family":"Oliva","sequence":"first","affiliation":[{"name":"Aix Marseille University, IP-TPT UMR MD3, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Flori","sequence":"additional","affiliation":[{"name":"Service des Agents Infectieux et Hygi\u00e8ne, CHU de Saint-Etienne, Saint-Etienne, France"},{"name":"Universit\u00e9 Jean Monnet, EA 3064-GIMAP, Saint-Etienne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Hennequin","sequence":"additional","affiliation":[{"name":"Sorbonne Universit\u00e9s, UPMC Univ. Paris 06, CR7, Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris), Paris, France"},{"name":"INSERM, U1135, CIMI-Paris, Paris, France"},{"name":"CNRS, ERL 8255, CIMI-Paris, Paris, France"},{"name":"AP-HP, H\u00f4pital St Antoine, Service de Parasitologie-Mycologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.-C.","family":"Dubus","sequence":"additional","affiliation":[{"name":"Pediatric Pulmonology Unit and Cystic Fibrosis Centre, APHM, CHU Timone, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Reynaud-Gaubert","sequence":"additional","affiliation":[{"name":"Department of Pulmonary Disease and Lung Transplantation, Competence Centre for Rare Pulmonary Diseases, APHM North Hospital, Aix Marseille University, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Charpin","sequence":"additional","affiliation":[{"name":"Clinique des Bronches, Allergie et Sommeil, H\u00f4pital Nord et INSERM U1067 CNRS UMR1067, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. M.","family":"Vergnon","sequence":"additional","affiliation":[{"name":"Service de Pneumologie et d'Oncologie Thoracique, CHU de Saint-Etienne, Saint-Etienne, France"},{"name":"Universit\u00e9 Jean Monnet, EA 4624-LINA, Saint-Etienne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Gay","sequence":"additional","affiliation":[{"name":"Service de Pneumologie et d'Oncologie Thoracique, CHU de Saint-Etienne, Saint-Etienne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Colly","sequence":"additional","affiliation":[{"name":"Service des Agents Infectieux et Hygi\u00e8ne, CHU de Saint-Etienne, Saint-Etienne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Piarroux","sequence":"additional","affiliation":[{"name":"Aix Marseille University, IP-TPT UMR MD3, Marseille, France"},{"name":"Parasitology-Mycology, APHM, CHU Timone, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Pelloux","sequence":"additional","affiliation":[{"name":"Parasitologie-Mycologie, D\u00e9partement des Agents Infectieux, CHU Grenoble and Universit\u00e9 Grenoble Alpes, Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3293-5276","authenticated-orcid":false,"given":"S.","family":"Ranque","sequence":"additional","affiliation":[{"name":"Aix Marseille University, IP-TPT UMR MD3, Marseille, France"},{"name":"Parasitology-Mycology, APHM, CHU Timone, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/MCP.0b013e328337d6de"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/hcm035"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2009.07.011"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.38.8.572"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780500064763"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mycmed.2012.01.004"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.07.002"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1159\/000232522"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12133"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00182-08"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00393-06"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2005.06.013"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.21240"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2012.08.006"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2013.10.016"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00200-10"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.25.1.81-84.1987"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.1.174-182.1991"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.12.2.304-309.2005"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-12-295"},{"key":"e_1_3_2_22_2","first-page":"1164","article-title":"Aspergillus antibody in patients with cystic fibrosis","volume":"129","author":"Bardana EJ","year":"1975","unstructured":"Bardana EJ, Jr, Sobti KL, Cianciulli FD, Noonan MJ. 1975. 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