{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T16:00:43Z","timestamp":1792166443232,"version":"4.4.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T00:00:00Z","timestamp":1364774400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Syndromic panels for infectious disease have been suggested to be of value in point-of-care diagnostics for developing countries and for biodefense. To test the performance of isothermal recombinase polymerase amplification (RPA) assays, we developed a panel of 10 RPAs for biothreat agents. The panel included RPAs for\n            <jats:named-content content-type=\"genus-species\">Francisella tularensis<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Yersinia pestis<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Bacillus anthracis<\/jats:named-content>\n            , variola virus, and reverse transcriptase RPA (RT-RPA) assays for Rift Valley fever virus, Ebola virus, Sudan virus, and Marburg virus. Their analytical sensitivities ranged from 16 to 21 molecules detected (probit analysis) for the majority of RPA and RT-RPA assays. A magnetic bead-based total nucleic acid extraction method was combined with the RPAs and tested using inactivated whole organisms spiked into plasma. The RPA showed comparable sensitivities to real-time RCR assays in these extracts. The run times of the assays at 42\u00b0C ranged from 6 to 10 min, and they showed no cross-detection of any of the target genomes of the panel nor of the human genome. The RPAs therefore seem suitable for the implementation of syndromic panels onto microfluidic platforms.\n          <\/jats:p>","DOI":"10.1128\/jcm.02704-12","type":"journal-article","created":{"date-parts":[[2013,1,24]],"date-time":"2013-01-24T09:51:49Z","timestamp":1359021109000},"page":"1110-1117","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":172,"title":["Development of a Panel of Recombinase Polymerase Amplification Assays for Detection of Biothreat Agents"],"prefix":"10.1128","volume":"51","author":[{"given":"Milena","family":"Euler","sequence":"first","affiliation":[{"name":"University Medical Center, Department of Virology, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjie","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory of Marine and Food Microbiology, College of Food Science and Technology, Shanghai Ocean University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris","family":"Heidenreich","sequence":"additional","affiliation":[{"name":"University Medical Center, Department of Virology, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pranav","family":"Patel","sequence":"additional","affiliation":[{"name":"Robert Koch Institute, Center for Biological Security 1, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Strohmeier","sequence":"additional","affiliation":[{"name":"University of Freiburg, Institute of Microsystem Technology, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sydney","family":"Hakenberg","sequence":"additional","affiliation":[{"name":"University of Freiburg, Institute of Microsystem Technology, Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Niedrig","sequence":"additional","affiliation":[{"name":"Robert Koch Institute, Center for Biological Security 1, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank T.","family":"Hufert","sequence":"additional","affiliation":[{"name":"University Medical Center, Department of Virology, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Weidmann","sequence":"additional","affiliation":[{"name":"University Medical Center, Department of Virology, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2010.03279.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2006.09.003"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1553-2712.2010.00790.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(04)01044-8"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.334"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2008.08.020"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2005.12.006"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1385\/MB:19:1:029"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1039\/c0lc00666a"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/15257770701845204"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.4155\/bio.10.172"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1039\/c1ib00049g"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/26.9.2150"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gnf065"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040204"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.2144\/000112959"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7400200"},{"key":"e_1_3_3_19_2","first-page":"29","volume-title":"Sensortechnik aktuell 2007","author":"Haberstroh K","year":"2007","unstructured":"HaberstrohK ReiffE-C . 2007. 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