{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:59:03Z","timestamp":1791853143920,"version":"4.3.4"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2013,3,1]],"date-time":"2013-03-01T00:00:00Z","timestamp":1362096000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The persistence of dormant, noncultivable\n            <jats:named-content content-type=\"genus-species\">Borrelia burgdorferi<\/jats:named-content>\n            after ceftriaxone treatment was examined.\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            isolates were cultivated in Barbour-Stoenner-Kelly medium in the presence or absence of ceftriaxone, and cultures were monitored for up to 56 days. Viability of\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            was assessed by subculture, growth, morphology, and pH (as a surrogate for metabolic activity). In addition, the presence of\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            DNA and mRNA was assayed by PCR and by real-time reverse transcription (RT)-PCR, respectively. Spirochetes could not be successfully subcultured by day 3 after exposure to ceftriaxone. In cultures treated with ceftriaxone, the pH of the culture medium did not change through day 56, whereas it declined by at least 1 pH unit by 14 days in untreated cultures. These results suggest that\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            viability is rapidly eliminated after antibiotic treatment. Nevertheless, DNA was detected by\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            -specific PCR for up to 56 days in aliquots from both ceftriaxone-treated and untreated cultures. In addition, although ceftriaxone treatment resulted in a reduction in the quantities of transcript for\n            <jats:italic>ospC<\/jats:italic>\n            ,\n            <jats:italic>ospA<\/jats:italic>\n            ,\n            <jats:italic>flaB<\/jats:italic>\n            , and\n            <jats:italic>pfk<\/jats:italic>\n            , certain mRNAs could be detected through day 14. Transcript for all 4 genes was essentially undetectable after 28 days of treatment. Taken together, the results suggest that\n            <jats:named-content content-type=\"genus-species\">B. burgdorferi<\/jats:named-content>\n            DNA and mRNA can be detected in samples long after spirochetes are no longer viable as assessed by classic microbiological parameters. PCR positivity in the absence of culture positivity following antibiotic treatment in animal and human studies should be interpreted with caution.\n          <\/jats:p>","DOI":"10.1128\/jcm.02785-12","type":"journal-article","created":{"date-parts":[[2012,12,27]],"date-time":"2012-12-27T01:34:56Z","timestamp":1356572096000},"page":"857-862","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["Detection of Borrelia burgdorferi Nucleic Acids after Antibiotic Treatment Does Not Confirm Viability"],"prefix":"10.1128","volume":"51","author":[{"given":"Radha","family":"Iyer","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Department of Medicine, New York Medical College, Valhalla, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priyanka","family":"Mukherjee","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Department of Medicine, New York Medical College, Valhalla, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kemeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Department of Medicine, New York Medical College, Valhalla, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"Simons","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Department of Medicine, New York Medical College, Valhalla, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary P.","family":"Wormser","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, New York Medical College, Valhalla, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ira","family":"Schwartz","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Department of Medicine, New York Medical College, Valhalla, New York, USA"},{"name":"Division of Infectious Diseases, Department of Medicine, New York Medical College, Valhalla, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"624","article-title":"Final 2011 reports of nationally notifiable diseases","volume":"61","author":"Centers for Disease Control and Prevention","year":"2012","unstructured":"Centers for Disease Control and Prevention. 2012. 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