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However, recent serial testing studies of low-risk individuals have revealed higher false conversion rates with IGRAs than with TST. Reproducibility studies have identified various sources of variability that contribute to nonreproducible results. Sources of variability can be broadly classified as preanalytical, analytical, postanalytical, manufacturing, and immunological. In this minireview, we summarize known sources of variability and their impact on IGRA results. We also provide recommendations on how to minimize sources of IGRA variability.<\/jats:p>","DOI":"10.1128\/jcm.02803-15","type":"journal-article","created":{"date-parts":[[2016,1,14]],"date-time":"2016-01-14T09:57:12Z","timestamp":1452765432000},"page":"845-850","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":96,"title":["Interferon Gamma Release Assays for Latent Tuberculosis: What Are the Sources of Variability?"],"prefix":"10.1128","volume":"54","author":[{"given":"Niaz","family":"Banaei","sequence":"first","affiliation":[{"name":"Department of Pathology, Stanford University School of Medicine, Stanford, California, USA"},{"name":"Department of Medicine, Division of Infectious Diseases and Geographic Medicine, Stanford University School of Medicine, Stanford, California, USA"},{"name":"Clinical Microbiology Laboratory, Stanford Health Care, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajiv L.","family":"Gaur","sequence":"additional","affiliation":[{"name":"Department of Pathology, Stanford University School of Medicine, Stanford, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madhukar","family":"Pai","sequence":"additional","affiliation":[{"name":"McGill International TB Centre and Department of Epidemiology &amp; Biostatistics, McGill University, Montreal, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00034-13"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)60177-4"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1513\/AnnalsATS.201405-188OC"},{"key":"e_1_3_2_5_2","first-page":"A4735","article-title":"Affect of blood collection time on Quantiferon-TB Gold In-Tube test variability","volume":"185","author":"Mazurek GH","year":"2012","unstructured":"Mazurek GH, Whitworth WC, Goodwin DJ. 2012. 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