{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T22:32:22Z","timestamp":1779921142988,"version":"3.53.1"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T00:00:00Z","timestamp":1364774400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Human papillomavirus (HPV) epidemiological and vaccine studies require highly sensitive HPV detection and genotyping systems. To improve HPV detection by PCR, the broad-spectrum L1-based SPF\n            <jats:sub>10<\/jats:sub>\n            PCR DNA enzyme immunoassay (DEIA) LiPA system and a novel E6-based multiplex type-specific system (MPTS123) that uses Luminex xMAP technology were combined into a new testing algorithm. To evaluate this algorithm, cervical swabs (\n            <jats:italic>n<\/jats:italic>\n            = 860) and cervical biopsy specimens (\n            <jats:italic>n<\/jats:italic>\n            = 355) were tested, with a focus on HPV types detected by the MPTS123 assay (types 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, 68, 6, and 11). Among the HPV-positive samples, identifications of individual HPV genotypes were compared. When all MPTS123 targeted genotypes were considered together, good overall agreement was found (\u03ba = 0.801, 95% confidence interval [CI], 0.784 to 0.818) with identification by SPF\n            <jats:sub>10<\/jats:sub>\n            LiPA, but significantly more genotypes (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001) were identified by the MPTS123 PCR Luminex assay, especially for HPV types 16, 35, 39, 45, 58, and 59. An alternative type-specific assay was evaluated that is based on detection of a limited number of HPV genotypes by type-specific PCR and a reverse hybridization assay (MPTS12 RHA). This assay showed results similar to those of the expanded MPTS123 Luminex assay. These results confirm the fact that broad-spectrum PCRs are hampered by type competition when multiple HPV genotypes are present in the same sample. Therefore, a testing algorithm combining the broad-spectrum PCR and a range of type-specific PCRs can offer a highly accurate method for the analysis of HPV infections and diminish the rate of false-negative results and may be particularly useful for epidemiological and vaccine studies.\n          <\/jats:p>","DOI":"10.1128\/jcm.02831-12","type":"journal-article","created":{"date-parts":[[2013,1,31]],"date-time":"2013-01-31T03:54:41Z","timestamp":1359604481000},"page":"1171-1178","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["A Human Papilloma Virus Testing Algorithm Comprising a Combination of the L1 Broad-Spectrum SPF\n            <sub>10<\/sub>\n            PCR Assay and a Novel E6 High-Risk Multiplex Type-Specific Genotyping PCR Assay"],"prefix":"10.1128","volume":"51","author":[{"given":"Dirk","family":"van Alewijk","sequence":"first","affiliation":[{"name":"DDL Diagnostic Laboratory, Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Kleter","sequence":"additional","affiliation":[{"name":"DDL Diagnostic Laboratory, Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maarten","family":"Vent","sequence":"additional","affiliation":[{"name":"DDL Diagnostic Laboratory, Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marc","family":"Delroisse","sequence":"additional","affiliation":[{"name":"GlaxoSmithKline Vaccines, Rixensart, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurits","family":"de Koning","sequence":"additional","affiliation":[{"name":"DDL Diagnostic Laboratory, Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leen-Jan","family":"van Doorn","sequence":"additional","affiliation":[{"name":"DDL Diagnostic Laboratory, Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wim","family":"Quint","sequence":"additional","affiliation":[{"name":"DDL Diagnostic Laboratory, Rijswijk, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"Colau","sequence":"additional","affiliation":[{"name":"GlaxoSmithKline Vaccines, Rixensart, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.3.745-747.1996"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.02.002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.5.3074-3083.1995"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-76-4-1057"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70230-8"},{"key":"e_1_3_2_7_2","first-page":"121","article-title":"Polymerase chain reaction-based methods for the detection of human papillomavirus DNA","volume":"119","author":"Gravitt PE","year":"1992","unstructured":"GravittPE ManosMM . 1992. 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