{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T00:54:57Z","timestamp":1790297697714,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2016,3,25]],"date-time":"2016-03-25T00:00:00Z","timestamp":1458864000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,4,1]],"date-time":"2016-04-01T00:00:00Z","timestamp":1459468800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We developed and evaluated a multiplex antibody detection-based immunoassay for the diagnosis of prosthetic joint infections (PJIs). Sixteen protein antigens from three\n            <jats:named-content content-type=\"genus-species\">Staphylococcus<\/jats:named-content>\n            species (\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Staphylococcus epidermidis<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Staphylococcus lugdunensis<\/jats:named-content>\n            ) (8 antigens),\n            <jats:named-content content-type=\"genus-species\">Streptococcus agalactiae<\/jats:named-content>\n            (4 antigens), and\n            <jats:named-content content-type=\"genus-species\">Propionibacterium acnes<\/jats:named-content>\n            (4 antigens) were selected by comparative immunoproteomics using serum samples from PJI cases versus controls. A bead-based multiplex immunoassay that measured serum IgG against purified, recombinant forms of each of the 16 antigens was developed. We conducted a prospective study to evaluate the performance of the assay. A PJI was defined by the presence of a sinus tract and\/or positive intraoperative sample cultures (at least one sample yielding a virulent organism or at least two samples yielding the same organism). A total of 455 consecutive patients undergoing revision or resection arthroplasty (hip, 66.3%; knee, 29.7%; shoulder, 4%) at two French reference centers for the management of PJI were included: 176 patients (38.7%) were infected and 279 (61.3%) were not. About 60% of the infections involved at least one of the species targeted by the assay. The sensitivity\/specificity values were 72.3%\/80.7% for targeted staphylococci, 75%\/92.6% for\n            <jats:named-content content-type=\"genus-species\">S. agalactiae<\/jats:named-content>\n            , and 38.5%\/84.8% for\n            <jats:named-content content-type=\"genus-species\">P. acnes<\/jats:named-content>\n            . The assay was more sensitive for infections occurring &gt;3 months after arthroplasty and for patients with an elevated C-reactive protein (CRP) or erythrocyte sedimentation rate (ESR). However, it detected 64.3% and 58.3% of targeted staphylococcal infections associated with normal CRP and ESR values, respectively. This new multiplex immunoassay approach is a novel noninvasive tool to evaluate patients suspected of having PJIs and provides information complementary to that from inflammatory marker values.\n          <\/jats:p>","DOI":"10.1128\/jcm.02885-15","type":"journal-article","created":{"date-parts":[[2016,2,11]],"date-time":"2016-02-11T20:33:22Z","timestamp":1455222802000},"page":"1065-1073","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Multiplex Antibody Detection for Noninvasive Genus-Level Diagnosis of Prosthetic Joint Infection"],"prefix":"10.1128","volume":"54","author":[{"given":"Simon","family":"Marmor","sequence":"first","affiliation":[{"name":"Service de Chirurgie Orthop\u00e9dique, Groupe Hospitalier Diaconesses Croix Saint-Simon, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Bauer","sequence":"additional","affiliation":[{"name":"Service de Chirurgie Orthop\u00e9dique et Traumatologie, H\u00f4pital Ambroise Par\u00e9 (Assistance Publique\u2013H\u00f4pitaux de Paris [AP-HP]), Boulogne-Billancourt, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Desplaces","sequence":"additional","affiliation":[{"name":"Service de Microbiologie, Groupe Hospitalier Diaconesses Croix Saint-Simon, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beate","family":"Heym","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie, H\u00f4pital Ambroise Par\u00e9 (AP-HP), Boulogne-Billancourt, France"},{"name":"UMR 1173, UFR Simone Veil, Universit\u00e9 de Versailles Saint-Quentin-en-Yvelines, Montigny-le-Bretonneux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Laure","family":"Roux","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie, H\u00f4pital Ambroise Par\u00e9 (AP-HP), Boulogne-Billancourt, France"},{"name":"UMR 1173, UFR Simone Veil, Universit\u00e9 de Versailles Saint-Quentin-en-Yvelines, Montigny-le-Bretonneux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Sol","sequence":"additional","affiliation":[{"name":"DIAXONHIT, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie","family":"Rog\u00e9","sequence":"additional","affiliation":[{"name":"DIAXONHIT, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Mah\u00e9","sequence":"additional","affiliation":[{"name":"DIAXONHIT, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"D\u00e9sir\u00e9","sequence":"additional","affiliation":[{"name":"DIAXONHIT, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Aegerter","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche Clinique Paris \u00cele-de-France Ouest, H\u00f4pital Ambroise Par\u00e9 (AP-HP), Boulogne-Billancourt, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Idir","family":"Ghout","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche Clinique Paris \u00cele-de-France Ouest, H\u00f4pital Ambroise Par\u00e9 (AP-HP), Boulogne-Billancourt, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Ropers","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche Clinique Paris \u00cele-de-France Ouest, H\u00f4pital Ambroise Par\u00e9 (AP-HP), Boulogne-Billancourt, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Louis","family":"Gaillard","sequence":"additional","affiliation":[{"name":"Laboratoire de Microbiologie, H\u00f4pital Ambroise Par\u00e9 (AP-HP), Boulogne-Billancourt, France"},{"name":"UMR 1173, UFR Simone Veil, Universit\u00e9 de Versailles Saint-Quentin-en-Yvelines, Montigny-le-Bretonneux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5953-3344","authenticated-orcid":false,"given":"Martin","family":"Rottman","sequence":"additional","affiliation":[{"name":"UMR 1173, UFR Simone Veil, Universit\u00e9 de Versailles Saint-Quentin-en-Yvelines, Montigny-le-Bretonneux, France"},{"name":"Laboratoire de Microbiologie, H\u00f4pital Raymond Poincar\u00e9 (AP-HP), Garches, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","unstructured":". 2010. 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