{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T02:11:26Z","timestamp":1790475086047,"version":"4.1.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            This study aimed to evaluate the repeated sequence REP-529 compared to that of the B1 gene in the molecular diagnosis of toxoplasmosis by quantitative PCR (qPCR) in routine diagnosis. Over a 10-year period (2003 to 2013), all patients prospectively diagnosed with a positive REP-529 qPCR result for toxoplasmosis were included. All DNA samples (76 samples from 56 patients) were simultaneously tested using the two qPCR methods (REP-529 and B1). The mean cycle threshold (\n            <jats:italic>\n              C\n              <jats:sub>T<\/jats:sub>\n            <\/jats:italic>\n            ) obtained with the B1 qPCR was significantly higher (+4.71 cycles) than that obtained with REP-529 qPCR (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001). Thirty-one out of 69 extracts (45.6%) positive with REP-529 qPCR were not amplified with the B1 qPCR (relative sensitivity of 54.4% compared to that with REP-529), yielding false-negative results with 15\/28 placenta, 5 cord blood, 2 amniotic fluid, 4 cerebrospinal fluid, 1 aqueous humor, 2 lymph node puncture, and 1 abortion product sample. This defect in sensitivity would have left 20\/56 patients undiagnosed, distributed as follows: 12\/40 congenital toxoplasmosis, 4\/5 cerebral toxoplasmosis, 2\/8 patients with retinochoroiditis, and 2 patients with chronic lymphadenopathy. This poor performance of B1 qPCR might be related to low parasite loads, since the mean\n            <jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>\n            quantification in extracts with B1 false-negative results was 0.4 parasite\/reaction. These results clearly show the superiority of the REP-529 sequence in the diagnosis of toxoplasmosis by PCR and suggest that this target should be adopted as part of the standardization of the PCR assay.\n          <\/jats:p>","DOI":"10.1128\/jcm.02900-14","type":"journal-article","created":{"date-parts":[[2015,2,5]],"date-time":"2015-02-05T05:09:34Z","timestamp":1423112974000},"page":"1294-1300","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":74,"title":["A 10-Year Retrospective Comparison of Two Target Sequences, REP-529 and B1, for Toxoplasma gondii Detection by Quantitative PCR"],"prefix":"10.1128","volume":"53","author":[{"given":"Sorya","family":"Belaz","sequence":"first","affiliation":[{"name":"INSERM U1085, Institut de Recherche en Sant\u00e9 Environnement et Travail, Universit\u00e9 Rennes 1, Rennes, France"},{"name":"Laboratoire de Parasitologie et Mycologie, Centre Hospitalier Universitaire de Rennes, Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre","family":"Gangneux","sequence":"additional","affiliation":[{"name":"INSERM U1085, Institut de Recherche en Sant\u00e9 Environnement et Travail, Universit\u00e9 Rennes 1, Rennes, France"},{"name":"Laboratoire de Parasitologie et Mycologie, Centre Hospitalier Universitaire de Rennes, Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peggy","family":"Dupretz","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie et Mycologie, Centre Hospitalier Universitaire de Rennes, Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Guiguen","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie et Mycologie, Centre Hospitalier Universitaire de Rennes, Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Robert-Gangneux","sequence":"additional","affiliation":[{"name":"INSERM U1085, Institut de Recherche en Sant\u00e9 Environnement et Travail, Universit\u00e9 Rennes 1, Rennes, France"},{"name":"Laboratoire de Parasitologie et Mycologie, Centre Hospitalier Universitaire de Rennes, Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16412-X"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.05013-11"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.9.2893-2898.1999"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0029-7844(00)01118-2"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00918-12"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e3181b20ed1"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e318123e8d3"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2011.06.006"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.8.1787-1792.1989"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1590\/0037-8682-0095-2013"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(99)00170-8"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-3-7"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01900-07"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.3.720-724.2006"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.10.4826-4828.2003"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1970130619"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2008.09.009"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2013.02.008"},{"key":"e_1_3_1_20_2","first-page":"541","article-title":"Retrospective evaluation of the detection of Toxoplasma gondii by polymerase chain reaction in AIDS patients","volume":"25","author":"Robert F","year":"1996","unstructured":"Robert F, Ouatas T, Blanche P, Tourte-Schaefer C, Sicard D, Dupouy-Camet J. 1996. Retrospective evaluation of the detection of Toxoplasma gondii by polymerase chain reaction in AIDS patients. Presse Med 25:541\u2013545. 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