{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T06:21:28Z","timestamp":1762323688056},"reference-count":11,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T00:00:00Z","timestamp":1396310400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A loop-mediated isothermal amplification (LAMP) system, the\n            <jats:italic>illumi<\/jats:italic>\n            gene Mycoplasma DNA amplification assay (Meridian Bioscience, Inc., Cincinnati, OH) was evaluated to determine its analytical sensitivity, specificity, and clinical application in comparison to historic culture in a collection of archived respiratory specimens. The\n            <jats:italic>illumi<\/jats:italic>\n            gene limit of detection was \u226488 CFU\/reaction for 10\n            <jats:named-content content-type=\"genus-species\">Mycoplasma pneumoniae<\/jats:named-content>\n            reference strains. This assay correctly identified 36\n            <jats:named-content content-type=\"genus-species\">M. pneumoniae<\/jats:named-content>\n            reference strains and clinical isolates from various geographic origins, including both of the main subtypes. No cross-reactions were detected with other mycoplasmas, ureaplasmas, other bacterial species, viruses, yeasts, or human DNA. Among 214 respiratory specimens previously cultured for\n            <jats:named-content content-type=\"genus-species\">M. pneumoniae<\/jats:named-content>\n            , when real-time PCR with bidirectional sequencing of the PCR products was used to resolve discrepancies, the sensitivity was 22 of 22 (100%) and the specificity was 190 of 192 (99%). This commercial LAMP assay is a useful rapid method for detecting\n            <jats:named-content content-type=\"genus-species\">M. pneumoniae<\/jats:named-content>\n            in clinical specimens. Additional prospective clinical trials with direct comparison to culture and PCR are warranted.\n          <\/jats:p>","DOI":"10.1128\/jcm.02913-13","type":"journal-article","created":{"date-parts":[[2014,1,16]],"date-time":"2014-01-16T02:49:38Z","timestamp":1389840578000},"page":"1060-1063","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Comparison of the\n            <i>illumi<\/i>\n            gene Mycoplasma DNA Amplification Assay and Culture for Detection of Mycoplasma pneumoniae"],"prefix":"10.1128","volume":"52","author":[{"given":"Amy E.","family":"Ratliff","sequence":"first","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynn B.","family":"Duffy","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken B.","family":"Waites","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Alabama at Birmingham, Birmingham, Alabama, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.2217\/17460913.3.6.635"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"e_1_3_3_4_2","volume-title":"Nolte FS","author":"Waites KB","year":"2001","unstructured":"WaitesKBBebearCMRobertsonJATalkingtonDFKennyGE. 2001. Cumitech 34: laboratory diagnosis of mycoplasmal infections. Nolte FS (ed). American Society for Microbiology, Washington, DC."},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5921"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46071-0"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-012-0388-5"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2616-2622.2003"},{"key":"e_1_3_3_9_2","first-page":"53","article-title":"Usefulness of the Loopamp Mycoplasma P detecting reagent kit developed based on the LAMP method","volume":"23","author":"Matsuda C","year":"2013","unstructured":"MatsudaCTaketaniTTakeuchiSTaniguchiYNagiraMMoriyamaHShibataHNagaiA. 2013. Usefulness of the Loopamp Mycoplasma P detecting reagent kit developed based on the LAMP method. Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi. 23:53\u201359. (In Japanese.)","journal-title":"Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi."},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00321-07"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1139\/w05-118"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.11.18"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02913-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02913-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:57:01Z","timestamp":1646481421000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02913-13"}},"subtitle":[],"editor":[{"given":"K. C.","family":"Carroll","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2014,4]]},"references-count":11,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["10.1128\/JCM.02913-13"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02913-13","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4]]},"assertion":[{"value":"2013-10-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-01-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-03-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}