{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:31:23Z","timestamp":1787016683705,"version":"build-2736575974"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2015,3,1]],"date-time":"2015-03-01T00:00:00Z","timestamp":1425168000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,3]]},"abstract":"ABSTRACT<\/jats:title>\n \n Macrolide resistance has been linked to the presence of a functional erythromycin ribosomal methylase (\n erm<\/jats:named-content>\n ) gene in most species of pathogenic rapidly growing mycobacteria (RGM). For these\n Mycobacterium<\/jats:named-content>\n isolates, extended incubation in clarithromycin is necessary to determine macrolide susceptibility. In contrast, the absence of a detectable\n erm<\/jats:named-content>\n gene in isolates of\n M. chelonae<\/jats:named-content>\n ,\n M. senegalense<\/jats:named-content>\n , and\n M. peregrinum<\/jats:named-content>\n and a nonfunctional\n erm<\/jats:named-content>\n gene in\n M. abscessus<\/jats:named-content>\n subsp.\n massiliense<\/jats:named-content>\n and 15% to 20% of\n M. abscessus<\/jats:named-content>\n subsp.\n abscessus<\/jats:named-content>\n isolates renders these species intrinsically macrolide susceptible. Not all RGM species have been screened for the presence of an\n erm<\/jats:named-content>\n gene, including the\n Mycobacterium mucogenicum<\/jats:named-content>\n group (\n M. mucogenicum<\/jats:named-content>\n ,\n M. phocaicum<\/jats:named-content>\n , and\n M. aubagnense<\/jats:named-content>\n ) and\n Mycobacterium immunogenum<\/jats:named-content>\n . A total of 356 isolates of these two pathogenic RGM taxa from two reference laboratories (A.R.U.P. Reference Laboratories and the Mycobacteria\/Nocardia Laboratory at the University of Texas Health Science Center at Tyler) underwent clarithromycin susceptibility testing with readings at 3 to 5 days and 14 days. Only 13 of the 356 isolates had resistant clarithromycin MICs at initial extended MIC readings, and repeat values on all available isolates were \u22642 \u03bcg\/ml. These studies suggest that these two additional RGM groups do not harbor functional\n erm<\/jats:named-content>\n genes and, like\n M. chelonae<\/jats:named-content>\n , do not require extended clarithromycin susceptibility testing. We propose to the Clinical Laboratory and Standards Institute that isolates belonging to these above-mentioned six rapidly growing mycobacterial groups based on molecular identification with no known functional\n erm<\/jats:named-content>\n genes undergo only 3 to 5 days of susceptibility testing (to exclude mutational resistance).\n <\/jats:p>","DOI":"10.1128\/jcm.02936-14","type":"journal-article","created":{"date-parts":[[2015,1,7]],"date-time":"2015-01-07T23:41:40Z","timestamp":1420674100000},"page":"875-878","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Absence of a Functional erm Gene in Isolates of Mycobacterium immunogenum and the Mycobacterium mucogenicum Group, Based on\n In Vitro<\/i>\n Clarithromycin Susceptibility"],"prefix":"10.1128","volume":"53","author":[{"given":"Barbara A.","family":"Brown-Elliott","sequence":"first","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, The University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly","family":"Hanson","sequence":"additional","affiliation":[{"name":"Pathology Laboratories, A.R.U.P., Salt Lake City, Utah, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sruthi","family":"Vasireddy","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, The University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Iakhiaeva","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, The University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin A.","family":"Nash","sequence":"additional","affiliation":[{"name":"Department of Pathology, Keck School of Medicine of USC, Los Angeles, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravikiran","family":"Vasireddy","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, The University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Parodi","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, The University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terry","family":"Smith","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, The University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha","family":"Gee","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, The University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita","family":"Strong","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, The University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Barker","sequence":"additional","affiliation":[{"name":"Pathology Laboratories, A.R.U.P., Salt Lake City, Utah, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Cohen","sequence":"additional","affiliation":[{"name":"Pathology Laboratories, A.R.U.P., Salt Lake City, Utah, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haleina","family":"Muir","sequence":"additional","affiliation":[{"name":"Pathology Laboratories, A.R.U.P., Salt Lake City, Utah, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. Susan","family":"Slechta","sequence":"additional","affiliation":[{"name":"Pathology Laboratories, A.R.U.P., Salt Lake City, Utah, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Richard J.","family":"Wallace","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, The University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Approved standard M24-A2","author":"Clinical and Laboratory Standards Institute","year":"2011","unstructured":"Clinical and Laboratory Standards Institute. 2011. Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes, 2nd ed. Approved standard M24-A2. Clinical and Laboratory Standards Institute, Wayne, PA.","edition":"2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00402-06"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01275-08"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkh523"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00861-10"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2010.00221.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03464-13"},{"key":"e_1_3_2_9_2","volume-title":"Abstr 114th Gen Meet Am Soc Microbiol. American Society for Microbiology","author":"Brown-Elliott BA","year":"2014","unstructured":"Brown-Elliott BA, Vasireddy S, Vasireddy R, Iakhiaeva E, Parodi N, Strong A, Gee M, McNulty S, O'Neil S, Wallace RJ, Jr. 2014. Sequencing of the erm gene in isolates of Mycobacterium abscessus subspecies abscessus with low clarithromycin MICs, abstr U-945. Abstr 114th Gen Meet Am Soc Microbiol. American Society for Microbiology, Washington, DC."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63969-0"},{"key":"e_1_3_2_11_2","volume-title":"Approved standard MM18-A","author":"Clinical and Laboratory Standards Institute","year":"2008","unstructured":"Clinical and Laboratory Standards Institute. 2008. Interpretive criteria for identification of bacteria and fungi by DNA target sequencing, 1st ed. Approved standard MM18-A. 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A.","family":"Forbes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,3]]},"references-count":20,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,3]]}},"alternative-id":["10.1128\/JCM.02936-14"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02936-14","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3]]},"assertion":[{"value":"2014-10-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-12-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-02-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}