{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T23:35:55Z","timestamp":1781134555759,"version":"3.54.1"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>The control of swine influenza virus (SIV) infection is paramount for increasing the productivity of pig farming and minimizing the threat of pandemic outbreaks. Thus, SIV surveillance should be conducted by region and on a regular basis. Here, we established a microneutralization assay specific for SIV seroprevalence surveillance by using reporter gene-expressing recombinant influenza viruses. Growth-based SIV seroprevalence revealed that most sows and piglets were positive for neutralizing antibodies against influenza viruses. In contrast, the 90-day-old growing pigs exhibited limited neutralizing activity in their sera, suggesting that this particular age of population is most susceptible to SIV infection and thus is an ideal age group for SIV isolation. From nasal swab specimens of healthy pigs in this age population, we were able to isolate SIVs at a higher incidence (5.3%) than those of previous reports. Nucleotide sequencing and phylogenetic analysis of the hemagglutinin (HA) genes revealed that the isolated SIVs have circulated and evolved in pigs but not have been recently introduced from humans, implying that a large number of SIV lineages may remain \u201cundiscovered\u201d in the global porcine populations. We propose that the 90-day-old growing pig-targeted nasal swab collection presented in this study facilitates global SIV surveillance and contributes to the detection and control of SIV infection.<\/jats:p>","DOI":"10.1128\/jcm.02941-14","type":"journal-article","created":{"date-parts":[[2015,2,19]],"date-time":"2015-02-19T09:34:13Z","timestamp":1424338453000},"page":"1331-1338","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Efficient Isolation of Swine Influenza Viruses by Age-Targeted Specimen Collection"],"prefix":"10.1128","volume":"53","author":[{"given":"Makoto","family":"Ozawa","sequence":"first","affiliation":[{"name":"Laboratory of Animal Hygiene, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan"},{"name":"Transboundary Animal Diseases Center, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan"},{"name":"United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aya","family":"Matsuu","sequence":"additional","affiliation":[{"name":"Transboundary Animal Diseases Center, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan"},{"name":"United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kouki","family":"Yonezawa","sequence":"additional","affiliation":[{"name":"Department of Computer Bioscience, Nagahama Institute of Bio-Science and Technology, Nagahama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manabu","family":"Igarashi","sequence":"additional","affiliation":[{"name":"Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan"},{"name":"Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kosuke","family":"Okuya","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Hygiene, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshiko","family":"Kawabata","sequence":"additional","affiliation":[{"name":"Transboundary Animal Diseases Center, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimihito","family":"Ito","sequence":"additional","affiliation":[{"name":"Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan"},{"name":"Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyoko","family":"Tsukiyama-Kohara","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Hygiene, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan"},{"name":"Transboundary Animal Diseases Center, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan"},{"name":"United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Taneno","sequence":"additional","affiliation":[{"name":"Vaxxinova Japan K.K., Tokyo, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eisaburo","family":"Deguchi","sequence":"additional","affiliation":[{"name":"Transboundary Animal Diseases Center, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan"},{"name":"United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi, Japan"},{"name":"Laboratory of Farm Animal Production Medicine, Joint Faculty of Veterinary Medicine, Kagoshima University, Kagoshima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.1978.tb01062.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.10.8851-8856.1999"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(00)00154-4"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1135(95)00145-X"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2003.11.005"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(85)90131-X"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-75-9-2183"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1993.1155"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature08182"},{"key":"e_1_3_2_11_2","first-page":"1691","volume-title":"Fields virology","author":"Wright PF","year":"2007","unstructured":"Wright PF, Neumann G, Kawaoka Y. 2007. Orthomyxoviruses, p. 1691\u20131740. In Knipe DM, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B, Straus SE (ed), Fields virology, 5th, vol 2. Lippincott Williams & Wilkins, Philadelphia, PA.","edition":"5"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2006062"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2012.00501.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.037648-0"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.8.4139-4146.2005"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.16.9345"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-013-1932-z"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0076753"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0057684"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst197"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.1980050306"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-animal-031412-103733"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.19.9322-9327.2000"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01475-12"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.06824-11"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1709.110338"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid\/1706.101886"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11262-011-0597-5"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.028662-0"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10004"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05352-11"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00053-10"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00306-1"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1906.121637"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-8-537"},{"key":"e_1_3_2_36_2","first-page":"363","article-title":"Infection of pigs with an influenza virus related to the A2-Hong Kong-1-68 strain","volume":"16","author":"Popovici V","year":"1972","unstructured":"Popovici V, Hiastru F, Draghici D, Zilisteanu E, Matepiuc M, Cretescu L, Niculescu I. 1972. Infection of pigs with an influenza virus related to the A2-Hong Kong-1-68 strain. Acta Virol 16:363.","journal-title":"Acta Virol"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-77-8-1751"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-004-0324-9"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01310021"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01314719"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-75-2-371"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12152"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02941-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02941-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:18:33Z","timestamp":1646482713000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02941-14"}},"subtitle":[],"editor":[{"given":"B. W.","family":"Fenwick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,4]]},"references-count":41,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["10.1128\/JCM.02941-14"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02941-14","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4]]},"assertion":[{"value":"2014-10-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-01-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-03-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}