{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:10:23Z","timestamp":1790694623847,"version":"4.1.0"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2016,5,1]],"date-time":"2016-05-01T00:00:00Z","timestamp":1462060800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            This multicenter study analyzed\n            <jats:named-content content-type=\"genus-species\">Nocardia<\/jats:named-content>\n            spp., including extraction, spectral acquisition, Bruker matrix-assisted laser desorption ionization\u2013time of flight mass spectrometry (MALDI-TOF MS) identification, and score interpretation, using three\n            <jats:named-content content-type=\"genus-species\">Nocardia<\/jats:named-content>\n            libraries, the Bruker, National Institutes of Health (NIH), and The Ohio State University (OSU) libraries, and compared the results obtained by each center. A standardized study protocol, 150\n            <jats:named-content content-type=\"genus-species\">Nocardia<\/jats:named-content>\n            isolates, and NIH and OSU\n            <jats:named-content content-type=\"genus-species\">Nocardia<\/jats:named-content>\n            MALDI-TOF MS libraries were distributed to three centers. Following standardized culture, extraction, and MALDI-TOF MS analysis, isolates were identified using score cutoffs of \u22652.0 for species\/species complex-level identification and \u22651.8 for genus-level identification. Isolates yielding a score of &lt;2.0 underwent a single repeat extraction and analysis. The overall score range for all centers was 1.3 to 2.7 (average, 2.2 \u00b1 0.3), with common species generally producing higher average scores than less common ones. Score categorization and isolate identification demonstrated 86% agreement between centers; 118 of 150 isolates were correctly identified to the species\/species complex level by all centers. Nine strains (6.0%) were not identified by any center, and six (4.0%) of these were uncommon species with limited library representation. A categorical score discrepancy among centers occurred for 21 isolates (14.0%). There was an overall benefit of 21.2% from repeat extraction of low-scoring isolates and a center-dependent benefit for duplicate spotting (range, 2 to 8.7%). Finally, supplementation of the Bruker\n            <jats:named-content content-type=\"genus-species\">Nocardia<\/jats:named-content>\n            MALDI-TOF MS library with both the OSU and NIH libraries increased the genus-level and species-level identification by 18.2% and 36.9%, respectively. Overall, this study demonstrates the ability of diverse clinical microbiology laboratories to utilize MALDI-TOF MS for the rapid identification of clinically relevant\n            <jats:named-content content-type=\"genus-species\">Nocardia<\/jats:named-content>\n            spp. and to implement MALDI-TOF MS libraries developed by single laboratories across institutions.\n          <\/jats:p>","DOI":"10.1128\/jcm.02942-15","type":"journal-article","created":{"date-parts":[[2016,2,25]],"date-time":"2016-02-25T04:36:33Z","timestamp":1456374993000},"page":"1251-1258","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Multicenter Matrix-Assisted Laser Desorption Ionization\u2013Time of Flight Mass Spectrometry Study for Identification of Clinically Relevant Nocardia spp"],"prefix":"10.1128","volume":"54","author":[{"given":"Sara J.","family":"Blosser","sequence":"first","affiliation":[{"name":"National Institutes of Health, Department of Laboratory Medicine, Microbiology Service, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven K.","family":"Drake","sequence":"additional","affiliation":[{"name":"National Institutes of Health, Critical Care Medicine Department, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer L.","family":"Andrasko","sequence":"additional","affiliation":[{"name":"Cleveland Clinic, Pathology and Laboratory Medicine Institute, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina M.","family":"Henderson","sequence":"additional","affiliation":[{"name":"National Institutes of Health, Department of Laboratory Medicine, Microbiology Service, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamal","family":"Kamboj","sequence":"additional","affiliation":[{"name":"The Ohio State University Wexner Medical Center, Department of Pathology, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stella","family":"Antonara","sequence":"additional","affiliation":[{"name":"National Institutes of Health, Department of Laboratory Medicine, Microbiology Service, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilia","family":"Mijares","sequence":"additional","affiliation":[{"name":"National Institutes of Health, Department of Laboratory Medicine, Microbiology Service, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Conville","sequence":"additional","affiliation":[{"name":"National Institutes of Health, Department of Laboratory Medicine, Microbiology Service, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen M.","family":"Frank","sequence":"additional","affiliation":[{"name":"National Institutes of Health, Department of Laboratory Medicine, Microbiology Service, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan M.","family":"Harrington","sequence":"additional","affiliation":[{"name":"Cleveland Clinic, Pathology and Laboratory Medicine Institute, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joan-Miquel","family":"Balada-Llasat","sequence":"additional","affiliation":[{"name":"The Ohio State University Wexner Medical Center, Department of Pathology, Columbus, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian M.","family":"Zelazny","sequence":"additional","affiliation":[{"name":"National Institutes of Health, Department of Laboratory Medicine, Microbiology Service, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.19.2.259-282.2006"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00155-06"},{"key":"e_1_3_3_4_2","first-page":"515","volume-title":"Manual of clinical microbiology","author":"Conville PS","year":"2007","unstructured":"Conville PS, Witebsky FB. 2007. Nocardia, Rhodococcus, Gordonia, Actinomadura, Streptomyces, and other aerobic actinomycetes, p 515\u2013542. In Murray PR, Baron EJ, Jorgensen JH, Landry ML, Pfaller MA (ed), Manual of clinical microbiology, 9th ed, vol 1. ASM Press, Washington, DC.","edition":"9"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.32.12.1776"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01531-13"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01234-10"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12014-015-9078-5"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjid.2014.11.003"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02680-12"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00780-15"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02135-10"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00567-15"},{"key":"e_1_3_3_14_2","volume-title":"Rapid identification of Nocardia species by pyrosequencing","author":"Farrell JJ","year":"2003","unstructured":"Farrell JJ, Tuohy MJ, Brown-Elliott BA, Patel JB, Wallace RJ, Jr, Hall GS, Procop GW. 2003. Rapid identification of Nocardia species by pyrosequencing, abstr 338. Abstr Annu Meet Infect Dis Soc Am, San Diego, CA."},{"key":"e_1_3_3_15_2","volume-title":"Abstr 104th Gen Meet Am Soc Microbiol","author":"Tuohy MJ","year":"2004","unstructured":"Tuohy MJ, Farrell JJ, Brown-Elliott BA, Mann L, Wilson RW, Wallace RJ, Jr, Hall GS, Procop GW. 2004. Evaluation of pyrosequencing for the identification of nocardia species., abstr 2578. Abstr 104th Gen Meet Am Soc Microbiol, New Orleans, LA. American Society for Microbiology, Washington, DC."},{"key":"e_1_3_3_16_2","volume-title":"Interpretive criteria for identification of bacteria and fungi by DNA target sequencing; approved guideline","author":"Clinical and Laboratory Standards Institute","year":"2008","unstructured":"Clinical and Laboratory Standards Institute. 2008. Interpretive criteria for identification of bacteria and fungi by DNA target sequencing; approved guideline. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02852-12"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02770-14"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1854"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02942-15","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02942-15","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T08:41:48Z","timestamp":1646469708000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02942-15"}},"subtitle":[],"editor":[{"given":"G. A.","family":"Land","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2016,5]]},"references-count":18,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2016,5]]}},"alternative-id":["10.1128\/JCM.02942-15"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02942-15","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5]]},"assertion":[{"value":"2015-11-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-02-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-04-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}