{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:59:42Z","timestamp":1791356382725,"version":"4.3.1"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n The\n erm<\/jats:italic>\n (41) gene confers inducible macrolide resistance in\n Mycobacterium abscessus<\/jats:named-content>\n subsp.\n abscessus<\/jats:named-content>\n , calling into question the usefulness of macrolides for treating\n M. abscessus<\/jats:named-content>\n subsp.\n abscessus<\/jats:named-content>\n infections. With an extended incubation (14 days), isolates with MICs of \u22658 \u03bcg\/ml are considered macrolide resistant by current CLSI guidelines. Our goals were to determine the incidence of macrolide susceptibility in U.S. isolates, the validity of currently accepted MIC breakpoints, and the\n erm<\/jats:italic>\n (41) sequences associated with susceptibility. Of 349 isolates (excluding those with 23S rRNA gene mutations), 85 (24%) had clarithromycin MICs of \u22648 \u03bcg\/ml. Sequencing of the\n erm<\/jats:italic>\n (41) genes from these isolates, as well as from isolates with MICs of \u226516 \u03bcg\/ml, including ATCC 19977\n T<\/jats:sup>\n , revealed 10 sequevars. The sequence in ATCC 19977\n T<\/jats:sup>\n was designated sequevar (type) 1; most macrolide-resistant isolates were of this type. Seven sequevars contained isolates with MICs of >16 \u03bcg\/ml. The T28C substitution in\n erm<\/jats:italic>\n (41), previously associated with macrolide susceptibility, was identified in 62 isolates (18%) comprising three sequevars, with MICs of \u22642 (80%), 4 (10%), and 8 (10%) \u03bcg\/ml. No other nucleotide substitution was associated with macrolide susceptibility. We recommend that clarithromycin susceptibility breakpoints for\n M. abscessus<\/jats:named-content>\n subsp.\n abscessus<\/jats:named-content>\n be changed from \u22642 to \u22644 \u03bcg\/ml and that isolates with an MIC of 8 \u03bcg\/ml have repeat MIC testing or\n erm<\/jats:italic>\n sequencing performed. Our studies suggest that macrolides are useful for treating approximately 20% of U.S. isolates of\n M. abscessus<\/jats:named-content>\n subsp.\n abscessus<\/jats:named-content>\n . Sequencing of the\n erm<\/jats:italic>\n gene of\n M. abscessus<\/jats:named-content>\n subsp.\n abscessus<\/jats:named-content>\n will predict inducible macrolide susceptibility.\n <\/jats:p>","DOI":"10.1128\/jcm.02950-14","type":"journal-article","created":{"date-parts":[[2015,2,5]],"date-time":"2015-02-05T05:09:34Z","timestamp":1423112974000},"page":"1211-1215","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":178,"title":["Utility of Sequencing the\n erm<\/i>\n (41) Gene in Isolates of Mycobacterium abscessus subsp. abscessus with Low and Intermediate Clarithromycin MICs"],"prefix":"10.1128","volume":"53","author":[{"given":"Barbara A.","family":"Brown-Elliott","sequence":"first","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sruthi","family":"Vasireddy","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ravikiran","family":"Vasireddy","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Iakhiaeva","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan T.","family":"Howard","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Nash","sequence":"additional","affiliation":[{"name":"Department of Pathology, Keck School of Medicine of USC, Los Angeles, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Parodi","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anita","family":"Strong","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martha","family":"Gee","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terry","family":"Smith","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Richard J.","family":"Wallace","sequence":"additional","affiliation":[{"name":"Mycobacteria\/Nocardia Laboratory, University of Texas Health Science Center at Tyler, Tyler, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00861-10"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2010.00221.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2003.131106"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01275-08"},{"key":"e_1_3_2_6_2","volume-title":"Abstr 114th Gen Meet Am Soc Microbiol. American Society for Microbiology","author":"Brown-Elliott BA","year":"2014","unstructured":"Brown-Elliott BA, Vasireddy S, Vasireddy R, Iakhiaeva E, Parodi N, Strong A, Gee M, McNulty S, O'Neil S, Wallace RJ, Jr. 2014. Sequencing of the erm gene in isolates of Mycobacterium abscessus subspecies abscessus with low clarithromycin MICs, abstr 945. Abstr 114th Gen Meet Am Soc Microbiol. American Society for Microbiology, Washington, DC."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63969-0"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.1.149-153.1995"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.2.175-178.1993"},{"key":"e_1_3_2_10_2","volume-title":"CLSI document M24-A2","author":"Clinical and Laboratory Standards Institute","year":"2011","unstructured":"Clinical and Laboratory Standards Institute. 2011. Susceptibility testing of mycobacteria, nocardiae, and other aerobic actinomycetes: approved standard\u20142nd ed. CLSI document M24-A2. CLSI, Wayne, PA."},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.40.7.1676"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00402-06"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/174.2.354"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.2.381"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(98)00013-3"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200604-571ST"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01132-13"}],"updated-by":[{"DOI":"10.1128\/jcm.00192-16","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2016,3,25]],"date-time":"2016-03-25T00:00:00Z","timestamp":1458864000000}},{"DOI":"10.1128\/jcm.00887-25","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000}}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02950-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02950-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:19:31Z","timestamp":1646482771000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02950-14"}},"subtitle":[],"editor":[{"given":"B. A.","family":"Forbes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,4]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["10.1128\/JCM.02950-14"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02950-14","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4]]},"assertion":[{"value":"2014-10-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-01-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-03-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}