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In this study, different gene target PCR-restriction fragment length polymorphism (RFLP) protocols were evaluated for their robustness as\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            species discriminators in 61 patients with cutaneous leishmaniasis. We modified the\n            <jats:italic>hsp70<\/jats:italic>\n            -PCR-RFLP protocol and found it to be the most reliable protocol for species identification.\n          <\/jats:p>","DOI":"10.1128\/jcm.02955-14","type":"journal-article","created":{"date-parts":[[2015,1,22]],"date-time":"2015-01-22T06:01:02Z","timestamp":1421906462000},"page":"1339-1341","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Improving Leishmania Species Identification in Different Types of Samples from Cutaneous Lesions"],"prefix":"10.1128","volume":"53","author":[{"given":"M\u00f3nica L.","family":"Cruz-Barrera","sequence":"first","affiliation":[{"name":"Laboratorio de Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional de Colombia, Bogot\u00e1, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clemencia","family":"Ovalle-Bracho","sequence":"additional","affiliation":[{"name":"Centro Dermatol\u00f3gico Federico Lleras Acosta, Bogot\u00e1, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viviana","family":"Ortegon-Vergara","sequence":"additional","affiliation":[{"name":"Centro Dermatol\u00f3gico Federico Lleras Acosta, Bogot\u00e1, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jairo E.","family":"P\u00e9rez-Franco","sequence":"additional","affiliation":[{"name":"Hospital Militar Central, Bogot\u00e1, Colombia"},{"name":"Departamento de Medicina Interna, Facultad de Medicina, Universidad Nacional de Colombia, Bogot\u00e1, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria C.","family":"Echeverry","sequence":"additional","affiliation":[{"name":"Laboratorio de Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional de Colombia, Bogot\u00e1, Colombia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03640.x"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/524042"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.19.1.111-126.2006"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/503371"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002871"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1998.59.86"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2010.09-0414"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0049738"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/648730"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2013-0098-OA"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(03)90031-9"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(03)00040-3"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/1745-6215-13-58"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.5.2294-2297.2004"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182010000089"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1990.42.206"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.7705\/biomedica.v26i1.1508"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2010.08.007"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05061-11"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02955-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02955-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:21:40Z","timestamp":1646482900000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02955-14"}},"subtitle":[],"editor":[{"given":"B. 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