{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T03:42:54Z","timestamp":1789875774688,"version":"4.0.1"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2014,3,1]],"date-time":"2014-03-01T00:00:00Z","timestamp":1393632000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Trichomonas vaginalis<\/jats:named-content>\n            is the most prevalent nonviral sexually transmitted infection worldwide, and improved diagnostic methods are critical for controlling this pathogen. Diagnostic assays that can be used in conjunction with routine chlamydia\/gonorrhea nucleic acid-based screening are likely to have the most impact on disease control. Here we describe the performance of the new BD\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            Q\n            <jats:sup>x<\/jats:sup>\n            (TVQ) amplified DNA assay, which can be performed on the automated BD Viper system. We focus on data from vaginal swab samples, since this is the specimen type routinely used for traditional trichomonas testing and the recommended specimen type for chlamydia\/gonorrhea screening. Vaginal swabs were obtained from women attending sexually transmitted disease or family planning clinics at 7 sites. Patient-collected vaginal swabs were tested by the TVQ assay, and the Aptima\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            (ATV) assay was performed using clinician-collected vaginal swabs. Additional clinician-collected vaginal swabs were used for the wet mount and culture methods. Analyses included comparisons versus the patient infection status (PIS) defined by positive results with the wet mount method or culture, direct comparisons assessed with \u03ba scores, and latent class analysis (LCA) as an unbiased estimator of test accuracy. Data from 838 women, 116 of whom were infected with\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            , were analyzed. The TVQ assay sensitivity and specificity estimates based on the PIS were 98.3% and 99.0%, respectively. The TVQ assay was similar to the ATV assay (\u03ba = 0.938) in direct analysis. LCA estimated the TVQ sensitivity and specificity as 98.3 and 99.6%, respectively. The TVQ assay performed well using self-collected vaginal swabs, the optimal sample type, as recommended by the CDC for chlamydia\/gonorrhea screening among women.\n          <\/jats:p>","DOI":"10.1128\/jcm.02966-13","type":"journal-article","created":{"date-parts":[[2014,1,4]],"date-time":"2014-01-04T02:56:50Z","timestamp":1388804210000},"page":"885-889","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":59,"title":["Detection of Trichomonas vaginalis DNA by Use of Self-Obtained Vaginal Swabs with the BD ProbeTec Q\n            <sup>x<\/sup>\n            Assay on the BD Viper System"],"prefix":"10.1128","volume":"52","author":[{"given":"Barbara","family":"Van Der Pol","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, University of Alabama at Birmingham School of Medicine, Birmingham, Alabama, USA"},{"name":"Division of Infectious Diseases, Indiana University School of Medicine, Indianapolis, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James A.","family":"Williams","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Indiana University School of Medicine, Indianapolis, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie N.","family":"Taylor","sequence":"additional","affiliation":[{"name":"Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine L.","family":"Cammarata","sequence":"additional","affiliation":[{"name":"Louisiana State University Health Sciences Center, New Orleans, Louisiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles A.","family":"Rivers","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, University of Alabama at Birmingham School of Medicine, Birmingham, Alabama, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara A.","family":"Body","sequence":"additional","affiliation":[{"name":"Laboratory Corporation of America Holdings, Burlington, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melinda","family":"Nye","sequence":"additional","affiliation":[{"name":"Laboratory Corporation of America Holdings, Burlington, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deanna","family":"Fuller","sequence":"additional","affiliation":[{"name":"Wishard Health Services, Indianapolis, Indiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane R.","family":"Schwebke","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, University of Alabama at Birmingham School of Medicine, Birmingham, Alabama, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathilda","family":"Barnes","sequence":"additional","affiliation":[{"name":"Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte A.","family":"Gaydos","sequence":"additional","affiliation":[{"name":"Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"2008","volume-title":"Global incidence and prevalence of selected curable sexually transmitted diseases\u2014","author":"World Health Organization","year":"2012","unstructured":"World Health Organization. 2012. Global incidence and prevalence of selected curable sexually transmitted diseases\u20142008. World Health Organization, Geneva, Switzerland."},{"key":"e_1_3_3_3_2","volume-title":"Trichomoniasis: CDC fact sheet","author":"Centers for Disease Control and Prevention","year":"2012","unstructured":"Centers for Disease Control and Prevention. 2012. Trichomoniasis: CDC fact sheet. Centers for Disease Control and Prevention, Atlanta, GA."},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/522532"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-199707000-00008"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3016.2007.00807.x"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1067\/S0002-9378(03)00777-4"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/338399"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2012-050893"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2012-051005"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e3181cfcc4b"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e318186decf"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1086\/511278"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.7.4155-4160.2005"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.2003.tb00104.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1086\/526496"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1258\/095646203321043228"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2012-051001"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir705"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2008.10.005"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31820a94d2"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4749-4758.2004"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.2307\/2533043"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31827c08c3"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02966-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02966-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:44:28Z","timestamp":1646480668000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02966-13"}},"subtitle":[],"editor":[{"given":"P. H.","family":"Gilligan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2014,3]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,3]]}},"alternative-id":["10.1128\/JCM.02966-13"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02966-13","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3]]},"assertion":[{"value":"2013-10-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-12-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-02-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}