{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:18:42Z","timestamp":1787062722090,"version":"build-2736575974"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T00:00:00Z","timestamp":1623974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T00:00:00Z","timestamp":1623974400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100014089","name":"Boehringer Ingelheim France","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014089","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2021,6,18]]},"abstract":"<jats:p>\n                    Leptospirosis is a vaccine-preventable bacterial zoonotic disease caused by pathogenic\n                    <jats:named-content content-type=\"genus-species\">Leptospira<\/jats:named-content>\n                    species. The efficacy of\n                    <jats:italic>Leptospira<\/jats:italic>\n                    canine vaccines is assessed by challenging vaccinated and control dogs with virulent serovars of\n                    <jats:italic>Leptospira<\/jats:italic>\n                    , followed by detection of\n                    <jats:italic>Leptospira<\/jats:italic>\n                    in blood and urine.\n                  <\/jats:p>","DOI":"10.1128\/jcm.03006-20","type":"journal-article","created":{"date-parts":[[2021,4,15]],"date-time":"2021-04-15T08:09:38Z","timestamp":1618474178000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Quantitative Real-Time PCR Assays for the Detection of Pathogenic\n                    <i>Leptospira<\/i>\n                    Species in Urine and Blood Samples in Canine Vaccine Clinical Studies: a Rapid Alternative to Classical Culture Methods"],"prefix":"10.1128","volume":"59","author":[{"given":"Stephanie","family":"Blanchard","sequence":"first","affiliation":[{"name":"Boehringer Ingelheim Animal Health, Saint-Priest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carine","family":"Cariou","sequence":"additional","affiliation":[{"name":"Boehringer Ingelheim Animal Health, Saint-Priest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9r\u00f4me","family":"Bouvet","sequence":"additional","affiliation":[{"name":"Boehringer Ingelheim Animal Health, Saint-Vulbas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Valfort","sequence":"additional","affiliation":[{"name":"Boehringer Ingelheim Animal Health, Saint-Vulbas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frantz","family":"Oberli","sequence":"additional","affiliation":[{"name":"Boehringer Ingelheim Animal Health, Saint-Vulbas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9verine","family":"Villard","sequence":"additional","affiliation":[{"name":"Boehringer Ingelheim Animal Health, Saint-Priest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Barret-Hilaire","sequence":"additional","affiliation":[{"name":"Boehringer Ingelheim Animal Health, Saint-Priest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Poulet","sequence":"additional","affiliation":[{"name":"Boehringer Ingelheim Animal Health, Saint-Priest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lionel","family":"Cupillard","sequence":"additional","affiliation":[{"name":"Boehringer Ingelheim Animal Health, Saint-Priest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6367-7276","authenticated-orcid":true,"given":"Blandine","family":"de Saint-Vis","sequence":"additional","affiliation":[{"name":"Boehringer Ingelheim Animal Health, Saint-Priest, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.2.296-326.2001"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.03.012"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cvsm.2010.07.008"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/jsap.12328"},{"key":"e_1_3_3_6_2","first-page":"194","article-title":"The detection and characterization of pathogenic Leptospira and the use of OMPs as potential antigens and immunogens","volume":"23","author":"Gebriel AM","year":"2006","unstructured":"Gebriel AM, Subramaniam G, Sekaran SD. 2006. The detection and characterization of pathogenic Leptospira and the use of OMPs as potential antigens and immunogens. Trop Biomed 23:194\u2013207.","journal-title":"Trop Biomed"},{"key":"e_1_3_3_7_2","first-page":"67","article-title":"Development of PCR test system based on colA gene for detection of leptospirae in clinical material","volume":"5","author":"Vaganova AN","year":"2011","unstructured":"Vaganova AN, Stoianova NA, Tokarevich NK. 2011. Development of PCR test system based on colA gene for detection of leptospirae in clinical material. Zh Mikrobiol Epidemiol Immunobiol (5):67\u201371. (In Russian.)","journal-title":"Zh Mikrobiol Epidemiol Immunobiol"},{"key":"e_1_3_3_8_2","first-page":"9","article-title":"Rapid diagnosis of leptospirosis by multiplex PCR","volume":"19","author":"Ahmed SA","year":"2012","unstructured":"Ahmed SA, Sandai DA, Musa S, Hoe CH, Riadzi M, Lau KL, Tang TH. 2012. Rapid diagnosis of leptospirosis by multiplex PCR. Malays J Med Sci 19:9\u201316.","journal-title":"Malays J Med Sci"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vacrep.2016.07.003"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02452-10"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2009.06.009"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2018.11.001"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2019.109985"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02295996"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2010.01.003"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1177\/001316446002000104"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.2307\/2529310"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45860-0"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2005.06.011"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-2-13"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0132988"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.03006-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.03006-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T08:04:42Z","timestamp":1646467482000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.03006-20"}},"subtitle":[],"editor":[{"given":"Brad","family":"Fenwick","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,6,18]]},"references-count":21,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2021,6,18]]}},"alternative-id":["10.1128\/JCM.03006-20"],"URL":"https:\/\/doi.org\/10.1128\/jcm.03006-20","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,18]]},"assertion":[{"value":"2020-11-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03006-20"}}