{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T13:47:51Z","timestamp":1776260871607,"version":"3.50.1"},"reference-count":12,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T00:00:00Z","timestamp":1359676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Abilities to detect heterogeneity of\n            <jats:italic>ospC<\/jats:italic>\n            genotypes of the Lyme disease spirochete\n            <jats:named-content content-type=\"genus-species\">Borrelia burgdorferi<\/jats:named-content>\n            in the tick vector by\n            <jats:italic>in vitro<\/jats:italic>\n            culture (IVC) and direct PCR (dPCR) were compared. IVC failed to detect one-third of the\n            <jats:italic>ospC<\/jats:italic>\n            genotypes detected by dPCR. Among IVC results, common\n            <jats:italic>ospC<\/jats:italic>\n            genotypes were overrepresented while occurrence of rare genotypes was underestimated.\n          <\/jats:p>","DOI":"10.1128\/jcm.03009-12","type":"journal-article","created":{"date-parts":[[2012,11,23]],"date-time":"2012-11-23T03:50:19Z","timestamp":1353642619000},"page":"615-617","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Detection of Heterogeneity of Borrelia burgdorferi in Ixodes Ticks by Culture-Dependent and Culture-Independent Methods"],"prefix":"10.1128","volume":"51","author":[{"given":"Guang","family":"Xu","sequence":"first","affiliation":[{"name":"Laboratory of Medical Zoology, Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaime","family":"Wesker","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Zoology, Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caitlin","family":"White","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Zoology, Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jason","family":"Campbell","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Zoology, Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicholas G.","family":"Reich","sequence":"additional","affiliation":[{"name":"Division of Biostatistics and Epidemiology, School of Public Health and Health Sciences, University of Massachusetts, Amherst, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen M.","family":"Rich","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Zoology, Department of Microbiology, University of Massachusetts, Amherst, Massachusetts, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780470933961.ch9"},{"key":"e_1_3_2_3_2","first-page":"521","article-title":"Isolation and cultivation of Lyme disease spirochetes","volume":"57","author":"Barbour AG","year":"1984","unstructured":"BarbourAG. 1984. Isolation and cultivation of Lyme disease spirochetes. Yale J. Biol. Med. 57:521\u2013525.","journal-title":"Yale J. Biol. Med."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.5.1251-1255.1993"},{"key":"e_1_3_2_5_2","first-page":"112","article-title":"European Borrelia burgdorferi isolated from humans and ticks culture conditions and antibiotic susceptibility","volume":"263","author":"Preac-Mursic V","year":"1986","unstructured":"Preac-MursicV WilskeB SchierzG. 1986. European Borrelia burgdorferi isolated from humans and ticks culture conditions and antibiotic susceptibility. Zentralbl Bakteriol. Mikrobiol. Hyg. A 263:112\u2013118.","journal-title":"Zentralbl Bakteriol. Mikrobiol. Hyg. 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