{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T01:45:57Z","timestamp":1791078357199,"version":"4.1.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2013,6,1]],"date-time":"2013-06-01T00:00:00Z","timestamp":1370044800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Discrimination between\n                    <jats:named-content content-type=\"genus-species\">Streptococcus pneumoniae<\/jats:named-content>\n                    and its close relatives of the viridans group is a common difficulty in matrix-assisted laser desorption ionization\u2013time of flight (MALDI-TOF) mass spectrometry-based identification. In the present study, the performances of the Vitek MS MALDI-TOF mass spectrometry system were assessed using 334 pneumococci, 166 other\n                    <jats:named-content content-type=\"genus-species\">S. mitis<\/jats:named-content>\n                    group streptococci, 184 non-\n                    <jats:named-content content-type=\"genus-species\">S. mitis<\/jats:named-content>\n                    group streptococci, and 19 related alpha- and nonhemolytic aerobic Gram-positive catalase-negative coccal isolates. Pneumococci had been identified by means of optochin susceptibility and bile solubility or serotyping, and other isolates mainly by use of RapidID32 Strep strips. In case of discordant or low-discrimination results, genotypic methods were used. The sensitivity of the Vitek MS for the identification of\n                    <jats:named-content content-type=\"genus-species\">S. pneumoniae<\/jats:named-content>\n                    was 99.1%, since only three bile-insoluble isolates were misidentified as\n                    <jats:named-content content-type=\"genus-species\">Streptococcus mitis<\/jats:named-content>\n                    \/\n                    <jats:named-content content-type=\"genus-species\">Streptococcus oralis<\/jats:named-content>\n                    . Conversely, two optochin-resistant pneumococci were correctly identified (specificity, 100%). Three\n                    <jats:named-content content-type=\"genus-species\">Streptococcus pseudopneumoniae<\/jats:named-content>\n                    isolates were also correctly identified. Among nonpneumococcal isolates, 90.8% (\n                    <jats:italic>n<\/jats:italic>\n                    = 335) were correctly identified to the species or subspecies level and 2.4% (\n                    <jats:italic>n<\/jats:italic>\n                    = 9) at the group level. For the remaining 25 isolates, the Vitek MS proposed a bacterial species included in the list of possible species suggested by genotypic methods, except for 4 isolates which were not identified due to the absence of the species in the database. According to our study, the Vitek MS displays performance similar to that of the optochin susceptibility test for routine identification of pneumococcal isolates. Moreover, the Vitek MS is efficient for the identification of other viridans group streptococci and related isolates, provided that the species are included in the database.\n                  <\/jats:p>","DOI":"10.1128\/jcm.03069-12","type":"journal-article","created":{"date-parts":[[2013,4,10]],"date-time":"2013-04-10T23:29:53Z","timestamp":1365636593000},"page":"1861-1867","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["Identification of Clinical Streptococcus pneumoniae Isolates among other Alpha and Nonhemolytic Streptococci by Use of the Vitek MS Matrix-Assisted Laser Desorption Ionization\u2013Time of Flight Mass Spectrometry System"],"prefix":"10.1128","volume":"51","author":[{"given":"Damien","family":"Dubois","sequence":"first","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie-Hygi\u00e8ne, Institut F\u00e9d\u00e9ratif de Biologie, Centre Hospitalo-Universitaire, Toulouse, France"},{"name":"Universit\u00e9 Paul Sabatier-Toulouse III, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Segonds","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie-Hygi\u00e8ne, Institut F\u00e9d\u00e9ratif de Biologie, Centre Hospitalo-Universitaire, Toulouse, France"},{"name":"Universit\u00e9 Paul Sabatier-Toulouse III, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Fran\u00e7oise","family":"Prere","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie-Hygi\u00e8ne, Institut F\u00e9d\u00e9ratif de Biologie, Centre Hospitalo-Universitaire, Toulouse, France"},{"name":"Universit\u00e9 Paul Sabatier-Toulouse III, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Marty","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie-Hygi\u00e8ne, Institut F\u00e9d\u00e9ratif de Biologie, Centre Hospitalo-Universitaire, Toulouse, France"},{"name":"Universit\u00e9 Paul Sabatier-Toulouse III, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Oswald","sequence":"additional","affiliation":[{"name":"Laboratoire de Bact\u00e9riologie-Hygi\u00e8ne, Institut F\u00e9d\u00e9ratif de Biologie, Centre Hospitalo-Universitaire, Toulouse, France"},{"name":"Universit\u00e9 Paul Sabatier-Toulouse III, Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1128\/9781555816728.ch20","volume-title":"Manual of clinical microbiology","author":"Spellerberg B","year":"2011","unstructured":"SpellerbergB BrandtC. 2011. Streptococcus, p 331\u2013349. In VersalovicJ CarrollKC FunkeG JorgensenJH LandryML WarnockDW (ed), Manual of clinical microbiology, 10th ed. ASM Press, Washington, DC.","edition":"10"},{"key":"e_1_3_2_3_2","first-page":"428","article-title":"Regional pneumococcal observatories: antibiotic susceptibility and serotypes of Streptococcus pneumoniae isolated in France in 2009","volume":"2012","author":"Kempf M","year":"2012","unstructured":"KempfM GravetA, Observatoires regionaux du pneumocoque. 2012. Regional pneumococcal observatories: antibiotic susceptibility and serotypes of Streptococcus pneumoniae isolated in France in 2009. Bulletin Epidemiol. Hebd. 2012:428\u2013431.","journal-title":"Bulletin Epidemiol. Hebd"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-45-2-406"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.9.3373-3375.2001"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01137-06"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02013101"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0732-8893(90)90056-2"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02097-07"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/322803"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4686-4696.2004"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05182-11"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.39.8.871"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-10-104"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.10.3446-3451.2001"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.434-437.2000"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.017798-0"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2011.02.005"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(02)00051-9"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.9.4528-4534.2005"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46296-0"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.6.2651-2657.2004"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.2.497-504.2004"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1080\/09674845.2011.11730349"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2010.01913.x"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00365-12"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01450-10"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1198545"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2011"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002683"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03688.x"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01794-09"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.patbio.2009.07.020"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01881-09"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00343-12"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05971-11"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1086\/600885"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02071-09"},{"key":"e_1_3_2_39_2","first-page":"289","article-title":"Performance of a matrix-assisted laser desorption ionization-time-of-flight mass spectrometry system for the identification of bacterial isolates in the clinical routine laboratory","volume":"55","author":"Eigner U","year":"2009","unstructured":"EignerU HolfelderM OberdorferK Betz-WildU BertschD FahrAM. 2009. Performance of a matrix-assisted laser desorption ionization-time-of-flight mass spectrometry system for the identification of bacterial isolates in the clinical routine laboratory. Clin. Lab. 55:289\u2013296.","journal-title":"Clin. Lab"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00506-11"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016424"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.1.41-47.1998"},{"key":"e_1_3_2_43_2","first-page":"1043","article-title":"Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology","volume":"52","author":"Stackebrandt E","year":"2002","unstructured":"StackebrandtE FrederiksenW GarrityGM GrimontPA KampferP MaidenMC NesmeX Rossello-MoraR SwingsJ TruperHG VauterinL WardAC WhitmanWB. 2002. Report of the ad hoc committee for the re-evaluation of the species definition in bacteriology. Int. J. Syst. Evol. Microbiol. 52:1043\u20131047.","journal-title":"Int. J. Syst. Evol. Microbiol"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00413-09"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00508-12"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.3.923-927.2006"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.005488-0"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.018176-0"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-41-1-1"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2010.11.010"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-50-4-1425"},{"key":"e_1_3_2_52_2","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1099\/ijs.0.02361-0","article-title":"Reappraisal of the taxonomy of the Streptococcus bovis\/Streptococcus equinus complex and related species: description of Streptococcus gallolyticus subsp. gallolyticus subsp. nov., S. gallolyticus subsp. macedonicus subsp. nov. and S. gallolyticus subsp. pasteurianus subsp. nov","volume":"53","author":"Schlegel L","year":"2003","unstructured":"SchlegelL GrimontF AgeronE GrimontPA BouvetA. 2003. Reappraisal of the taxonomy of the Streptococcus bovis\/Streptococcus equinus complex and related species: description of Streptococcus gallolyticus subsp. gallolyticus subsp. nov., S. gallolyticus subsp. macedonicus subsp. nov. and S. gallolyticus subsp. pasteurianus subsp. nov. Int. J. Syst. Evol. Microbiol. 53:631\u2013645.","journal-title":"Int. J. Syst. Evol. Microbiol"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.03069-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.03069-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,6,30]],"date-time":"2023-06-30T19:20:59Z","timestamp":1688152859000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.03069-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6]]},"references-count":51,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2013,6]]}},"alternative-id":["10.1128\/JCM.03069-12"],"URL":"https:\/\/doi.org\/10.1128\/jcm.03069-12","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6]]},"assertion":[{"value":"2012-11-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-04-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-05-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}