{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T05:03:59Z","timestamp":1790485439641,"version":"4.1.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2014,3,1]],"date-time":"2014-03-01T00:00:00Z","timestamp":1393632000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,3]]},"abstract":"ABSTRACT<\/jats:title>\n \n Helcococcus<\/jats:named-content>\n spp. are Gram-positive, catalase-negative, facultatively anaerobic cocci that are associated with wound and prosthetic joint infections as well bacteremia and empyema. Five\n Helcococcus<\/jats:named-content>\n spp. strains were isolated from our patient population, including 2 strains of\n Helcococcus kunzii<\/jats:named-content>\n from trauma-associated wounds, 2\n Helcococcus sueciensis<\/jats:named-content>\n strains from blood and abscess, and a novel\n Helcococcus<\/jats:named-content>\n spp. strain from blood associated with urosepsis. Based on the phenotypic and phylogenetic evidence, we propose that the unknown bacterium be classified as\n Helcococcus seattlensis<\/jats:named-content>\n sp. nov. We found that all 5 tested\n Helcococcus<\/jats:named-content>\n strains grew as satellite colonies around\n Staphylococcus aureus<\/jats:named-content>\n and, interestingly, both\n H. kunzii<\/jats:named-content>\n strains were isolated together with\n S. aureus<\/jats:named-content>\n . In addition to 16S rRNA gene sequencing, conventional methods for leucine aminopeptidase (LAP) and pyrrolidonyl arylamidase (PYR) testing can be cost-effective and efficient for differentiation of\n Helcococcus<\/jats:named-content>\n spp. from\n Abiotrophia<\/jats:named-content>\n and\n Granulicatella<\/jats:named-content>\n species. Using nonstandard methods, we found that all tested\n Helcococcus<\/jats:named-content>\n spp. had high MICs of >4\/76 \u03bcg\/ml for trimethoprim-sulfamethoxazole, an antibiotic commonly used to treat urinary tract infections. High MICs for erythromycin, azithromycin, and clindamycin, and intermediate to high MICs for moxifloxacin, levofloxacin, and gentamicin were also observed among the\n Helcococcus<\/jats:named-content>\n strains.\n <\/jats:p>","DOI":"10.1128\/jcm.03076-13","type":"journal-article","created":{"date-parts":[[2013,12,27]],"date-time":"2013-12-27T02:40:00Z","timestamp":1388112000000},"page":"854-858","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Identification and Clinical Significance of Helcococcus species, with Description of Helcococcus seattlensis sp. nov. from a Patient with Urosepsis"],"prefix":"10.1128","volume":"52","author":[{"given":"Siu-Kei","family":"Chow","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"III","given":"Jill E.","family":"Clarridge","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, University of Washington, Seattle, Washington, USA"},{"name":"Veterans Administration Puget Sound Health Care System, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-43-3-425"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1009\/ijs.0.63077-0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-49-4-1429"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.12.6205-6208.2005"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01244-11"},{"key":"e_1_3_2_7_2","doi-asserted-by":"crossref","unstructured":"RiegelPLepargneurJP. 2003. Isolation of Helcococcus kunzii from a post-surgical foot abscess. Int. J. Med. 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Performance standards for antimicrobial susceptibility testing; twenty-third informational supplement. CLSI document M100-S23. 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