{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T10:44:09Z","timestamp":1761648249612},"reference-count":19,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2015,4,1]],"date-time":"2015-04-01T00:00:00Z","timestamp":1427846400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>We present here a rapid immunochromatographic assay for the detection of Middle East respiratory syndrome coronavirus (MERS-CoV) antigen in the nasal swabs of dromedary camels. The assay is based on the detection of MERS-CoV nucleocapsid protein in a short time frame using highly selective monoclonal antibodies at room temperature. The relative sensitivity and specificity of the assay were found to be 93.90% and 100%, respectively, compared to that of the UpE and open reading frame 1A (Orf1A) real-time reverse transcriptase PCR (RT-PCR). The results suggest that the assay developed here is a useful tool for the rapid diagnosis and epidemiological surveillance of MERS-CoV infection in dromedary camels.<\/jats:p>","DOI":"10.1128\/jcm.03096-14","type":"journal-article","created":{"date-parts":[[2015,1,29]],"date-time":"2015-01-29T05:12:11Z","timestamp":1422508331000},"page":"1178-1182","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Development and Validation of a Rapid Immunochromatographic Assay for Detection of Middle East Respiratory Syndrome Coronavirus Antigen in Dromedary Camels"],"prefix":"10.1128","volume":"53","author":[{"given":"Daesub","family":"Song","sequence":"first","affiliation":[{"name":"Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, University of Science and Technology, Yuseong-gu, Daejeon, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gunwoo","family":"Ha","sequence":"additional","affiliation":[{"name":"BioNote, Inc., Hwaseong-si, Gyeonggi-do, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wissam","family":"Serhan","sequence":"additional","affiliation":[{"name":"Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yassir","family":"Eltahir","sequence":"additional","affiliation":[{"name":"Epidemiology Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed","family":"Yusof","sequence":"additional","affiliation":[{"name":"Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farouq","family":"Hashem","sequence":"additional","affiliation":[{"name":"Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elsaeid","family":"Elsayed","sequence":"additional","affiliation":[{"name":"Veterinary Services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bahaaeldin","family":"Marzoug","sequence":"additional","affiliation":[{"name":"Veterinary Services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Assem","family":"Abdelazim","sequence":"additional","affiliation":[{"name":"Veterinary Services Section, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Salama","family":"Al Muhairi","sequence":"additional","affiliation":[{"name":"Veterinary Laboratories Division, Animal Wealth Sector, Abu Dhabi Food Control Authority, Abu Dhabi, United Arab Emirates"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1211721"},{"key":"e_1_3_2_3_2","volume-title":"Middle East respiratory syndrome coronavirus (MERS-CoV)\u2014update (United Arab Emirates)","author":"WHO","year":"2014","unstructured":"WHO. 2014. 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