{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T19:03:42Z","timestamp":1785179022234,"version":"3.55.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T00:00:00Z","timestamp":1364774400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Trichomonas vaginalis<\/jats:named-content>\n            infections are usually asymptomatic or can result in nonspecific clinical symptoms, which makes laboratory-based detection of this protozoan parasite essential for diagnosis and treatment. We report the development of a battery of highly sensitive and specific PCR assays for detection of\n            <jats:named-content content-type=\"genus-species\">T. vaginalis<\/jats:named-content>\n            in urine, a noninvasive specimen, and development of a protocol for differentiating among\n            <jats:named-content content-type=\"genus-species\">Trichomonas<\/jats:named-content>\n            species that commonly infect humans.\n          <\/jats:p>","DOI":"10.1128\/jcm.03101-12","type":"journal-article","created":{"date-parts":[[2013,2,7]],"date-time":"2013-02-07T04:51:23Z","timestamp":1360212683000},"page":"1298-1300","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Development of PCR Assays for Detection of Trichomonas vaginalis in Urine Specimens"],"prefix":"10.1128","volume":"51","author":[{"given":"Claudiu I.","family":"Bandea","sequence":"first","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kahaliah","family":"Joseph","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan W.","family":"Secor","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurie A.","family":"Jones","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph U.","family":"Igietseme","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L.","family":"Sautter","sequence":"additional","affiliation":[{"name":"Carolinas Pathology Group, Charlotte, North Carolina, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaret R.","family":"Hammerschlag","sequence":"additional","affiliation":[{"name":"SUNY Downstate Medical Center, Brooklyn, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy N.","family":"Fajman","sequence":"additional","affiliation":[{"name":"Emory University School of Medicine, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca G.","family":"Girardet","sequence":"additional","affiliation":[{"name":"The University of Texas\u2014Houston Medical School, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn M.","family":"Black","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Atlanta, Georgia, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e3282f3d999"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/522532"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/509934"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1363\/3600604"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.2.300"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/511278"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1086\/526496"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/423323"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/ije\/23.4.682"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.79.5.393"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2005.07.033"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.12.4127-4130.1999"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01447-06"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2008.10.005"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e31819b592e"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2008-2947"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpag.2010.03.004"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.2.366-373.2006"},{"key":"e_1_3_1_20_2","first-page":"819","article-title":"Trichomonas vaginalis: repeated DNA target for highly sensitive and specific polymerase chain reaction diagnosis","volume":"40","author":"Kengne P","year":"1994","unstructured":"KengneP VeasF VidalN ReyJL CunyG . 1994. 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