{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:17:48Z","timestamp":1790727468912,"version":"4.1.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Diagnosis of invasive aspergillosis (IA) remains challenging. With a relatively low incidence of disease, the use of expensive empirical antifungal therapy exposes many patients to unnecessary toxicity. Diagnosis places emphasis on specific but temporal radiological evidence. Circulating biomarker diagnosis has shown potential, but assays show variable performance, take several hours to perform, and require a degree of technical ability. A novel and simple lateral-flow device (LFD) using monoclonal antibody JF5, which targets an extracellular glycoprotein, has been developed and potentially removes any technical requirements, reducing processing time considerably. In this study, we evaluate the performance of this LFD compared to real-time PCR (targeting the 28S rRNA gene) and galactomannan (GM) detection when testing serum from a European Organization for Research and Treatment of Cancer\/Invasive Fungal Infections Cooperative Group, National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC\/MSG)-defined hematological population. In a proven\/probable-IA population versus a no-IA population, the LFD performance was comparable to that of both PCR and galactomannan enzyme immunoassay. Specificity (98.0%) was similar to that of PCR (96.6%) and slightly superior to that of GM (91.5%). Sensitivity (81.8%) was inferior to that of PCR (95.5%) but better than that of GM (77.3%). In combination with PCR, it provided both 100% sensitivity and 100% specificity. The LFD permits rapid testing of easily obtainable specimens, to be used as an adjunct test, before confirmation by other investigations. Its simplicity provides centers without specialist diagnostics with a test with clinical performance superior to that of classical microbiological approaches and results that can be used to direct antifungal management. In summary, microbiological diagnosis of IA is difficult and options are limited, with variable performance. An LFD assay targeting a novel specific biomarker has been developed, one which is methodologically simple and provides good clinical performance, particularly if combined with PCR.<\/jats:p>","DOI":"10.1128\/jcm.03189-12","type":"journal-article","created":{"date-parts":[[2013,3,14]],"date-time":"2013-03-14T05:24:52Z","timestamp":1363238692000},"page":"1510-1516","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":145,"title":["Evaluation of Real-Time PCR, Galactomannan Enzyme-Linked Immunosorbent Assay (ELISA), and a Novel Lateral-Flow Device for Diagnosis of Invasive Aspergillosis"],"prefix":"10.1128","volume":"51","author":[{"given":"P. Lewis","family":"White","sequence":"first","affiliation":[{"name":"Public Health Wales, Microbiology Cardiff, UHW, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Parr","sequence":"additional","affiliation":[{"name":"Public Health Wales, Microbiology Cardiff, UHW, Cardiff, United Kingdom"},{"name":"Infection, Immunity and Biochemistry, School of Medicine, Cardiff University, UHW, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Thornton","sequence":"additional","affiliation":[{"name":"University of Exeter, Exeter, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemary A.","family":"Barnes","sequence":"additional","affiliation":[{"name":"Infection, Immunity and Biochemistry, School of Medicine, Cardiff University, UHW, Cardiff, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1086\/588660","article-title":"Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer\/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC\/MSG) Consensus Group","volume":"46","author":"De Pauw B","year":"2008","unstructured":"De PauwB WalshTJ DonnellyJP StevensDA EdwardsJE CalandraT PappasPG MaertensJ LortholaryO KauffmanCA DenningDW PattersonTF MaschmeyerG BilleJ DismukesWE HerbrechtR HopeWW KibblerCC KullbergBJ MarrKA Mu\u00f1ozP OddsFC PerfectJR RestrepoA RuhnkeM SegalBH SobelJD SorrellTC ViscoliC WingardJR ZaoutisT BennettJE, European Organization for Research and Treatment of Cancer\/Invasive Fungal Infections Cooperative Group, National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC\/MSG) Consensus Group. 2008. Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer\/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC\/MSG) Consensus Group. Clin. Infect. Dis. 46:1813\u20131821.","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciq206"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD007394"},{"key":"e_1_3_2_5_2","first-page":"105","volume-title":"Aspergillosis: from diagnosis to prevention","author":"Maertens J","year":"2010","unstructured":"MaertensJ TheunissenK LagrouK. 2010. Galactomannan testing, p 105\u2013124. In PasqualottoAC (ed), Aspergillosis: from diagnosis to prevention, 1st ed. 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Understanding interobserver agreement: the kappa statistic. Fam. Med. 37:360\u2013363.","journal-title":"Fam. Med."},{"key":"e_1_3_2_19_2","first-page":"157","volume-title":"Lateral flow immunoassay.","author":"Faulstich K","year":"2009","unstructured":"FaulstichK GrulerR EberhardM LentzschD HaberstrohK. 2009. Handheld and portable reader devices for lateral flow immunoassays, p 157\u2013183. In WongRC TseHY (ed), Lateral flow immunoassay. Humana Press, New York, NY."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.5.1711-1718.2006"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.017418-0"},{"key":"e_1_3_2_22_2","first-page":"373","volume-title":"Aspergillus fumigatus and aspergillosis.","author":"Green R","year":"2009","unstructured":"GreenR ShibuyaK AndoT. 2009. Histology and radiology, p 373\u2013390. In LatgeJ-P SteinbachWJ (ed), Aspergillus fumigatus and aspergillosis. 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