{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T13:28:44Z","timestamp":1789910924309,"version":"4.0.1"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2013,5,1]],"date-time":"2013-05-01T00:00:00Z","timestamp":1367366400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The development of an accurate antigen detection assay for the diagnosis of active tuberculosis (TB) would represent a major clinical advance. Here, we demonstrate that the\n                    <jats:named-content content-type=\"genus-species\">Mycobacterium tuberculosis<\/jats:named-content>\n                    Rv1681 protein is a biomarker for active TB with potential diagnostic utility. We initially identified, by mass spectroscopy, peptides from the Rv1681 protein in urine specimens from 4 patients with untreated active TB. Rabbit IgG anti-recombinant Rv1681 detected Rv1681 protein in lysates and culture filtrates of\n                    <jats:named-content content-type=\"genus-species\">M. tuberculosis<\/jats:named-content>\n                    and immunoprecipitated it from pooled urine specimens from two TB patients. An enzyme-linked immunosorbent assay formatted with these antibodies detected Rv1681 protein in unconcentrated urine specimens from 11\/25 (44%) TB patients and 1\/21 (4.8%) subjects in whom TB was initially clinically suspected but then ruled out by conventional methods. Rv1681 protein was not detected in urine specimens from 10 subjects with\n                    <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n                    -positive urine cultures, 26 subjects with confirmed non-TB tropical diseases (11 with schistosomiasis, 5 with Chagas' disease, and 10 with cutaneous leishmaniasis), and 14 healthy subjects. These results provide strong validation of Rv1681 protein as a promising biomarker for TB diagnosis.\n                  <\/jats:p>","DOI":"10.1128\/jcm.03192-12","type":"journal-article","created":{"date-parts":[[2013,2,6]],"date-time":"2013-02-06T23:51:23Z","timestamp":1360194683000},"page":"1367-1373","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Validation of Mycobacterium tuberculosis Rv1681 Protein as a Diagnostic Marker of Active Pulmonary Tuberculosis"],"prefix":"10.1128","volume":"51","author":[{"given":"Nira R.","family":"Pollock","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Beth Israel Deaconess Medical Center, Boston, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilia","family":"Macovei","sequence":"additional","affiliation":[{"name":"Global Infectious Disease Research Center, The Forsyth Institute, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelly","family":"Kanunfre","sequence":"additional","affiliation":[{"name":"Global Infectious Disease Research Center, The Forsyth Institute, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rakesh","family":"Dhiman","sequence":"additional","affiliation":[{"name":"Global Infectious Disease Research Center, The Forsyth Institute, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blanca I.","family":"Restrepo","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, University of Texas Health Science Center Houston, Brownsville Regional Campus, Brownsville, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izelda","family":"Zarate","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, University of Texas Health Science Center Houston, Brownsville Regional Campus, Brownsville, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula A.","family":"Pino","sequence":"additional","affiliation":[{"name":"Division of Epidemiology, University of Texas Health Science Center Houston, Brownsville Regional Campus, Brownsville, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Mora-Guzman","sequence":"additional","affiliation":[{"name":"Secretaria de Salud de Tamaulipas, Matamoros, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo T.","family":"Fujiwara","sequence":"additional","affiliation":[{"name":"Federal University of Minas Gerais, Belo Horizonte, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerd","family":"Michel","sequence":"additional","affiliation":[{"name":"Foundation for Innovative New Diagnostics, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suely S.","family":"Kashino","sequence":"additional","affiliation":[{"name":"Global Infectious Disease Research Center, The Forsyth Institute, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Campos-Neto","sequence":"additional","affiliation":[{"name":"Global Infectious Disease Research Center, The Forsyth Institute, Cambridge, Massachusetts, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Global tuberculosis report 2012.","author":"World Health Organization","year":"2012","unstructured":"World Health Organization. 2012. Global tuberculosis report 2012. World Health Organization, Geneva, Switzerland."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jir860"},{"key":"e_1_3_2_4_2","volume-title":"Publication no. WHO\/HTM\/STB\/2010.2.","author":"World Health Organization","year":"2010","unstructured":"World Health Organization. 2010. The global plan to stop TB 2011-2015. Publication no. WHO\/HTM\/STB\/2010.2. 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