{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T23:31:16Z","timestamp":1792020676055,"version":"4.4.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2015,3,1]],"date-time":"2015-03-01T00:00:00Z","timestamp":1425168000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Azole resistance in\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            is increasingly reported. Here, we describe the validation of the AsperGenius, a new multiplex real-time PCR assay consisting of two multiplex real-time PCRs, one that identifies the clinically relevant\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            species, and one that detects the TR34, L98H, T289A, and Y121F mutations in CYP51A and differentiates susceptible from resistant\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            strains. The diagnostic performance of the AsperGenius assay was tested on 37 bronchoalveolar lavage (BAL) fluid samples from hematology patients and 40 BAL fluid samples from intensive care unit (ICU) patients using a BAL fluid galactomannan level of \u22651.0 or positive culture as the gold standard for detecting the presence of\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            . In the hematology and ICU groups combined, there were 22 BAL fluid samples from patients with invasive aspergillosis (IA) (2 proven, 9 probable, and 11 nonclassifiable). Nineteen of the 22 BAL fluid samples were positive, according to the gold standard. The optimal cycle threshold value for the presence of\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            was &lt;36. Sixteen of the 19 BAL fluid samples had a positive PCR (2\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            species and 14\n            <jats:named-content content-type=\"genus-species\">A. fumigatus<\/jats:named-content>\n            samples). This resulted in a sensitivity, specificity, and positive and negative predictive values of 88.9%, 89.3%, 72.7%, and 96.2%, respectively, for the hematology group and 80.0%, 93.3%, 80.0%, and 93.3%, respectively, in the ICU group. The CYP51A real-time PCR confirmed 12 wild-type and 2 resistant strains (1 TR34-L98H and 1 TR46-Y121F-T289A mutant). Voriconazole therapy failed for both patients. The AsperGenius multiplex real-time PCR assay allows for sensitive and fast detection of\n            <jats:named-content content-type=\"genus-species\">Aspergillus<\/jats:named-content>\n            species directly from BAL fluid samples. More importantly, this assay detects and differentiates wild-type from resistant strains, even if BAL fluid cultures remain negative.\n          <\/jats:p>","DOI":"10.1128\/jcm.03216-14","type":"journal-article","created":{"date-parts":[[2015,1,8]],"date-time":"2015-01-08T04:41:40Z","timestamp":1420692100000},"page":"868-874","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":149,"title":["Validation of a New Aspergillus Real-Time PCR Assay for Direct Detection of Aspergillus and Azole Resistance of Aspergillus fumigatus on Bronchoalveolar Lavage Fluid"],"prefix":"10.1128","volume":"53","author":[{"given":"Ga-Lai M.","family":"Chong","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Infectious Diseases, Erasmus Medical Center, Rotterdam, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy W. J.","family":"van de Sande","sequence":"additional","affiliation":[{"name":"Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Rotterdam, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gijs J. H.","family":"Dingemans","sequence":"additional","affiliation":[{"name":"PathoNostics B.V., Maastricht, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giel R.","family":"Gaajetaan","sequence":"additional","affiliation":[{"name":"PathoNostics B.V., Maastricht, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alieke G.","family":"Vonk","sequence":"additional","affiliation":[{"name":"Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Rotterdam, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Pierre","family":"Hayette","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, University Hospital of Li\u00e8ge (CHU), Li\u00e8ge, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis W. E.","family":"van Tegelen","sequence":"additional","affiliation":[{"name":"PathoNostics B.V., Maastricht, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guus F. M.","family":"Simons","sequence":"additional","affiliation":[{"name":"PathoNostics B.V., Maastricht, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bart J. A.","family":"Rijnders","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Infectious Diseases, Erasmus Medical Center, Rotterdam, the Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200401-093OC"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/525258"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/591531"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa020191"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200212263472618"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01092-06"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc061720"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03182-13"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00167-13"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dku009"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00231-09"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cit320"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02487-12"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq450"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00213-11"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt071"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1710.110226"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1086\/318483"},{"key":"e_1_3_3_20_2","unstructured":"Gaajetaan GR Dingemans GJH van de Sande WWJ Rijnders BJA Hayette MP Waber R van Tegelen DWE Simons G. 2014. A new diagnostic assay for the detection of Aspergillus fumigatus and its resistance pattern by real-time PCR; abstr 0216. 24th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) Barcelona 10 to 13th May 2014."},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1086\/657065"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1086\/595846"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.05026-11"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1086\/647935"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.03216-14","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.03216-14","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:07:20Z","timestamp":1646482040000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.03216-14"}},"subtitle":[],"editor":[{"given":"D. W.","family":"Warnock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2015,3]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,3]]}},"alternative-id":["10.1128\/JCM.03216-14"],"URL":"https:\/\/doi.org\/10.1128\/jcm.03216-14","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,3]]},"assertion":[{"value":"2014-11-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-12-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-02-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}