{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T15:54:59Z","timestamp":1789574099470,"version":"build-2803163510"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T00:00:00Z","timestamp":1623974400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2021,6,18]],"date-time":"2021-06-18T00:00:00Z","timestamp":1623974400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"MIZUHO MEDY Co., Ltd."}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2021,6,18]]},"abstract":"<jats:p>\n            The recent increase in macrolide-resistant\n            <jats:named-content content-type=\"genus-species\">Mycoplasma pneumoniae<\/jats:named-content>\n            in Asia has become a continuing problem. A point-of-care testing method that can quickly detect\n            <jats:named-content content-type=\"genus-species\">M. pneumoniae<\/jats:named-content>\n            and macrolide-resistant mutations (MR mutations) is critical for proper antimicrobial use.\n          <\/jats:p>","DOI":"10.1128\/jcm.03245-20","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T12:52:42Z","timestamp":1619441562000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Clinical Evaluation of a Novel Point-of-Care Assay To Detect Mycoplasma pneumoniae and Associated Macrolide-Resistant Mutations"],"prefix":"10.1128","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9995-5522","authenticated-orcid":true,"given":"Toshihiko","family":"Kakiuchi","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Faculty of Medicine, Saga University, Saga, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6989-6680","authenticated-orcid":true,"given":"Ippei","family":"Miyata","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Kawasaki Medical School, Kurashiki City, Okayama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reiji","family":"Kimura","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Kawasaki Medical School, Kurashiki City, Okayama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Goh","family":"Shimomura","sequence":"additional","affiliation":[{"name":"Society of Ambulatory and General Pediatrics of Fukuoka, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunihisa","family":"Shimomura","sequence":"additional","affiliation":[{"name":"Society of Ambulatory and General Pediatrics of Fukuoka, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Society of Ambulatory and General Pediatrics of Fukuoka, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takato","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Society of Ambulatory and General Pediatrics of Fukuoka, Fukuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunobu","family":"Ouchi","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Kawasaki Medical School, Kurashiki City, Okayama, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muneaki","family":"Matsuo","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Faculty of Medicine, Saga University, Saga, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(01)00147-5"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2015.20.37.30018"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1500245"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.4.697-728.2004"},{"key":"e_1_3_2_6_2","first-page":"1","article-title":"Atypical pneumonias in children","volume":"10","author":"Hammerschlag MR","year":"1995","unstructured":"Hammerschlag MR. 1995. Atypical pneumonias in children. Adv Pediatr Infect Dis 10:1\u201339.","journal-title":"Adv Pediatr Infect Dis"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2001.tb01293.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2310.170106"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.00385"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.02517-18"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.12.e63"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.6.e34"},{"key":"e_1_3_2_13_2","volume-title":"The 2017 guidelines for the management of respiratory infectious diseases in children in Japan.","author":"Japanese Society for Pediatric Infectious Diseases","year":"2017","unstructured":"Japanese Society for Pediatric Infectious Diseases. 2017. The 2017 guidelines for the management of respiratory infectious diseases in children in Japan. Japanese Society for Pediatric Infectious Diseases, Tokyo, Japan. http:\/\/www.jspid.jp\/pub\/sguideline.html#guideline2017. Accessed 6 May 2021."},{"key":"e_1_3_2_14_2","volume-title":"Guiding principles for treating Mycoplasma pneumoniae pneumonia.","author":"Japanese Society of Mycoplasmology","year":"2014","unstructured":"Japanese Society of Mycoplasmology. 2014. Guiding principles for treating Mycoplasma pneumoniae pneumonia. Japanese Society of Mycoplasmology, Tokyo, Japan. http:\/\/plaza.umin.ac.jp\/mycoplasma\/wp-content\/themes\/theme_jsm\/pdf\/eng_shisin.pdf. Accessed 6 May 2021."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-0733-5_9"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.001264"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40291-018-0360-x"},{"key":"e_1_3_2_18_2","volume-title":"Smart Gene.","author":"Mizuho Medy Co, Ltd","year":"2020","unstructured":"Mizuho Medy Co, Ltd. 2020. Smart Gene. Mizuho Medy Co, Ltd, Tosu, Saga, Japan. https:\/\/www.mizuho-m.co.jp\/product\/product_details\/000555.php. Accessed 30 November 2020."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.163.7.at1010"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0167-7012(00)00135-4"},{"key":"e_1_3_2_21_2","first-page":"7","article-title":"A feasible protocol for identifying macrolide resistant mutations in the 23S rRNA domain V sequence of Mycoplasma pneumoniae","volume":"47","author":"Miyata I","year":"2021","unstructured":"Miyata I, Ouchi K. 2021. A feasible protocol for identifying macrolide resistant mutations in the 23S rRNA domain V sequence of Mycoplasma pneumoniae. Kawasaki Med J 47:7\u201311.","journal-title":"Kawasaki Med J"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00448"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/ped.12513"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1843.2011.02102.x"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5520(05)70199-9"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00048-13"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2017.02.027"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0173635"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.12.4624-4630.2004"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/crj.12379"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.03245-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.03245-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T13:03:40Z","timestamp":1646485420000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.03245-20"}},"subtitle":[],"editor":[{"given":"Erik","family":"Munson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2021,6,18]]},"references-count":29,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2021,6,18]]}},"alternative-id":["10.1128\/JCM.03245-20"],"URL":"https:\/\/doi.org\/10.1128\/jcm.03245-20","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,18]]},"assertion":[{"value":"2020-12-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-04-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-06-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e03245-20"}}