{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:21:16Z","timestamp":1791570076223,"version":"4.3.4"},"reference-count":32,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2014,5,1]],"date-time":"2014-05-01T00:00:00Z","timestamp":1398902400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Mycoplasma genitalium<\/jats:named-content>\n            is a sexually transmitted organism commonly treated with azithromycin. However, macrolide resistance has been reported and is associated with point mutations in the 23S rRNA gene. To evaluate the prevalence of macrolide resistance in\n            <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n            isolates from clinical specimens from France, we first used a previously reported high-resolution melting assay. Because susceptible and resistant\n            <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n            isolates were hardly discriminated in\n            <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n            -positive clinical specimens, we developed a new molecular assay for the rapid detection of macrolide resistance. An assay using real-time PCR based on fluorescence resonance energy transfer (FRET) coupled with melting curve analysis was designed. The assay was first validated on characterized macrolide-resistant\n            <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n            isolates and then applied to 202 urogenital\n            <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n            -positive specimens collected from 178 patients from France in 2011 and 2012. Resistant genotypes were confirmed by 23S rRNA gene sequencing. Among the 202\n            <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n            -positive specimens, 155 were amplified, demonstrating a sensitivity of 76.7%. A substitution in the 23S rRNA gene was found in 14.2% of the patient samples. Nine and six patients had\n            <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n            isolates with a substitution at positions 2059 and 2058, respectively. In four cases, a mixed population of wild-type and mutated\n            <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n            isolates was observed. The prevalence of\n            <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n            macrolide resistance has been stable in France since its detection in 2006. Our FRET PCR assay is able to discriminate between wild-type and resistant genotypes directly from clinical specimens. This assay will allow clinicians to shorten the time to the initiation of effective disease treatment.\n          <\/jats:p>","DOI":"10.1128\/jcm.03318-13","type":"journal-article","created":{"date-parts":[[2014,2,27]],"date-time":"2014-02-27T05:11:14Z","timestamp":1393477874000},"page":"1549-1555","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":89,"title":["Direct Detection of Macrolide Resistance in Mycoplasma genitalium Isolates from Clinical Specimens from France by Use of Real-Time PCR and Melting Curve Analysis"],"prefix":"10.1128","volume":"52","author":[{"given":"Arabella","family":"Touati","sequence":"first","affiliation":[{"name":"Universit\u00e9 de Bordeaux, USC EA 3671, Mycoplasmal and Chlamydial Infections in Humans, Bordeaux, France"},{"name":"INRA, USC EA 3671, Mycoplasmal and Chlamydial Infections in Humans, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivia","family":"Peuchant","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Bordeaux, USC EA 3671, Mycoplasmal and Chlamydial Infections in Humans, Bordeaux, France"},{"name":"INRA, USC EA 3671, Mycoplasmal and Chlamydial Infections in Humans, Bordeaux, France"},{"name":"Laboratoire de Bact\u00e9riologie, Centre Hospitalier Universitaire de Bordeaux, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorgen S.","family":"Jensen","sequence":"additional","affiliation":[{"name":"Statens Serum Institute, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"B\u00e9b\u00e9ar","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Bordeaux, USC EA 3671, Mycoplasmal and Chlamydial Infections in Humans, Bordeaux, France"},{"name":"INRA, USC EA 3671, Mycoplasmal and Chlamydial Infections in Humans, Bordeaux, 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